2ckz

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
Line 1: Line 1:
-
[[Image:2ckz.gif|left|200px]]<br /><applet load="2ckz" size="350" color="white" frame="true" align="right" spinBox="true"
+
[[Image:2ckz.gif|left|200px]]
-
caption="2ckz, resolution 3.2&Aring;" />
+
 
-
'''X-RAY STRUCTURE OF RNA POLYMERASE III SUBCOMPLEX C17-C25.'''<br />
+
{{Structure
 +
|PDB= 2ckz |SIZE=350|CAPTION= <scene name='initialview01'>2ckz</scene>, resolution 3.2&Aring;
 +
|SITE=
 +
|LIGAND=
 +
|ACTIVITY= [http://en.wikipedia.org/wiki/DNA-directed_RNA_polymerase DNA-directed RNA polymerase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.7.6 2.7.7.6]
 +
|GENE=
 +
}}
 +
 
 +
'''X-RAY STRUCTURE OF RNA POLYMERASE III SUBCOMPLEX C17-C25.'''
 +
 
==Overview==
==Overview==
Line 7: Line 16:
==About this Structure==
==About this Structure==
-
2CKZ is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Active as [http://en.wikipedia.org/wiki/DNA-directed_RNA_polymerase DNA-directed RNA polymerase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.7.6 2.7.7.6] Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2CKZ OCA].
+
2CKZ is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2CKZ OCA].
==Reference==
==Reference==
-
Structural biology of RNA polymerase III: subcomplex C17/25 X-ray structure and 11 subunit enzyme model., Jasiak AJ, Armache KJ, Martens B, Jansen RP, Cramer P, Mol Cell. 2006 Jul 7;23(1):71-81. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=16818233 16818233]
+
Structural biology of RNA polymerase III: subcomplex C17/25 X-ray structure and 11 subunit enzyme model., Jasiak AJ, Armache KJ, Martens B, Jansen RP, Cramer P, Mol Cell. 2006 Jul 7;23(1):71-81. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/16818233 16818233]
[[Category: DNA-directed RNA polymerase]]
[[Category: DNA-directed RNA polymerase]]
[[Category: Protein complex]]
[[Category: Protein complex]]
Line 30: Line 39:
[[Category: trna synthesis]]
[[Category: trna synthesis]]
-
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 16:49:45 2008''
+
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 16:16:45 2008''

Revision as of 14:16, 20 March 2008

Image:2ckz.gif


PDB ID 2ckz

Drag the structure with the mouse to rotate
, resolution 3.2Å
Activity: DNA-directed RNA polymerase, with EC number 2.7.7.6
Coordinates: save as pdb, mmCIF, xml



X-RAY STRUCTURE OF RNA POLYMERASE III SUBCOMPLEX C17-C25.


Overview

We obtained an 11 subunit model of RNA polymerase (Pol) III by combining a homology model of the nine subunit core enzyme with a new X-ray structure of the subcomplex C17/25. Compared to Pol II, Pol III shows a conserved active center for RNA synthesis but a structurally different upstream face for specific initiation complex assembly during promoter selection. The Pol III upstream face includes a HRDC domain in subunit C17 that is translated by 35 A and rotated by 150 degrees compared to its Pol II counterpart. The HRDC domain is essential in vivo, folds independently in vitro, and, unlike other HRDC domains, shows no indication of nucleic acid binding. Thus, the HRDC domain is a functional module that could account for the role of C17 in Pol III promoter-specific initiation. During elongation, C17/25 may bind Pol III transcripts emerging from the adjacent exit pore, because the subcomplex binds to tRNA in vitro.

About this Structure

2CKZ is a Protein complex structure of sequences from Saccharomyces cerevisiae. Full crystallographic information is available from OCA.

Reference

Structural biology of RNA polymerase III: subcomplex C17/25 X-ray structure and 11 subunit enzyme model., Jasiak AJ, Armache KJ, Martens B, Jansen RP, Cramer P, Mol Cell. 2006 Jul 7;23(1):71-81. PMID:16818233

Page seeded by OCA on Thu Mar 20 16:16:45 2008

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/2ckz"
Personal tools