2cn2

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[[Image:2cn2.gif|left|200px]]<br /><applet load="2cn2" size="350" color="white" frame="true" align="right" spinBox="true"
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[[Image:2cn2.gif|left|200px]]
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caption="2cn2, resolution 2.10&Aring;" />
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'''CRYSTAL STRUCTURES OF CLOSTRIDIUM THERMOCELLUM XYLOGLUCANASE'''<br />
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{{Structure
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|PDB= 2cn2 |SIZE=350|CAPTION= <scene name='initialview01'>2cn2</scene>, resolution 2.10&Aring;
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|SITE= <scene name='pdbsite=AC1:Cd+Binding+Site+For+Chain+B'>AC1</scene>
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|LIGAND= <scene name='pdbligand=CD:CADMIUM ION'>CD</scene>
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|ACTIVITY= [http://en.wikipedia.org/wiki/Xyloglucan-specific_endo-beta-1,4-glucanase Xyloglucan-specific endo-beta-1,4-glucanase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.151 3.2.1.151]
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|GENE=
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}}
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'''CRYSTAL STRUCTURES OF CLOSTRIDIUM THERMOCELLUM XYLOGLUCANASE'''
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==Overview==
==Overview==
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==About this Structure==
==About this Structure==
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2CN2 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Clostridium_thermocellum Clostridium thermocellum] with <scene name='pdbligand=CD:'>CD</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Active as [http://en.wikipedia.org/wiki/Xyloglucan-specific_endo-beta-1,4-glucanase Xyloglucan-specific endo-beta-1,4-glucanase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.151 3.2.1.151] Known structural/functional Site: <scene name='pdbsite=AC1:Cd+Binding+Site+For+Chain+B'>AC1</scene>. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2CN2 OCA].
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2CN2 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Clostridium_thermocellum Clostridium thermocellum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2CN2 OCA].
==Reference==
==Reference==
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Crystal structures of Clostridium thermocellum xyloglucanase, XGH74A, reveal the structural basis for xyloglucan recognition and degradation., Martinez-Fleites C, Guerreiro CI, Baumann MJ, Taylor EJ, Prates JA, Ferreira LM, Fontes CM, Brumer H, Davies GJ, J Biol Chem. 2006 Aug 25;281(34):24922-33. Epub 2006 Jun 13. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=16772298 16772298]
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Crystal structures of Clostridium thermocellum xyloglucanase, XGH74A, reveal the structural basis for xyloglucan recognition and degradation., Martinez-Fleites C, Guerreiro CI, Baumann MJ, Taylor EJ, Prates JA, Ferreira LM, Fontes CM, Brumer H, Davies GJ, J Biol Chem. 2006 Aug 25;281(34):24922-33. Epub 2006 Jun 13. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/16772298 16772298]
[[Category: Clostridium thermocellum]]
[[Category: Clostridium thermocellum]]
[[Category: Single protein]]
[[Category: Single protein]]
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[[Category: xyloglucanase]]
[[Category: xyloglucanase]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 16:50:26 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 16:17:28 2008''

Revision as of 14:17, 20 March 2008

Image:2cn2.gif


PDB ID 2cn2

Drag the structure with the mouse to rotate
, resolution 2.10Å
Sites:
Ligands:
Activity: Xyloglucan-specific endo-beta-1,4-glucanase, with EC number 3.2.1.151
Coordinates: save as pdb, mmCIF, xml



CRYSTAL STRUCTURES OF CLOSTRIDIUM THERMOCELLUM XYLOGLUCANASE


Overview

The enzymatic degradation of the plant cell wall is central both to the natural carbon cycle and, increasingly, to environmentally friendly routes to biomass conversion, including the production of biofuels. The plant cell wall is a complex composite of cellulose microfibrils embedded in diverse polysaccharides collectively termed hemicelluloses. Xyloglucan is one such polysaccharide whose hydrolysis is catalyzed by diverse xyloglucanases. Here we present the structure of the Clostridium thermocellum xyloglucanase Xgh74A in both apo and ligand-complexed forms. The structures, in combination with mutagenesis data on the catalytic residues and the kinetics and specificity of xyloglucan hydrolysis reveal a complex subsite specificity accommodating seventeen monosaccharide moieties of the multibranched substrate in an open substrate binding terrain.

About this Structure

2CN2 is a Single protein structure of sequence from Clostridium thermocellum. Full crystallographic information is available from OCA.

Reference

Crystal structures of Clostridium thermocellum xyloglucanase, XGH74A, reveal the structural basis for xyloglucan recognition and degradation., Martinez-Fleites C, Guerreiro CI, Baumann MJ, Taylor EJ, Prates JA, Ferreira LM, Fontes CM, Brumer H, Davies GJ, J Biol Chem. 2006 Aug 25;281(34):24922-33. Epub 2006 Jun 13. PMID:16772298

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