This old version of Proteopedia is provided for student assignments while the new version is undergoing repairs. Content and edits done in this old version of Proteopedia after March 1, 2026 will eventually be lost when it is retired in about June of 2026.

Apply for new accounts at the new Proteopedia. Your logins will work in both the old and new versions.

2xog

From Proteopedia

(Difference between revisions)

Jump to: navigation, search

Revision as of 09:47, 25 December 2014

FUNCTIONAL AND STRUCTURAL ANALYSES OF N-ACYLSULFONAMIDE-LINKED DINUCLEOSIDE INHIBITORS OF RIBONUCLEASE A

Structural highlights

2xog is a 2 chain structure with sequence from Bos taurus. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Ligands:
Related:	1ssb, 1afu, 1w4q, 1c0b, 6rat, 1ymr, 1o0o, 8rsa, 5rsa, 2w5l, 1rno, 7rat, 1izq, 1eid, 1cjq, 1eos, 1rnn, 1j82, 1qhc, 1ymw, 1o0h, 1afk, 1f0v, 1dy5, 1rnq, 1jn4, 1rnm, 1rbf, 1z3m, 1rnx, 1rob, 1rbn, 1rbx, 1eow, 1rbi, 7rsa, 1rbj, 1dfj, 1rsm, 1kf5, 1xps, 1rat, 1fev, 2aas, 1rhb, 1j80, 3srn, 1b6v, 1fs3, 1rpg, 1z6s, 1kf8, 1rtb, 4rsk, 1bzq, 9rsa, 1bel, 1kf4, 1kf7, 1rbw, 9rat, 1eie, 1rpf, 1o0m, 1c8w, 1rnc, 1rca, 1c0c, 1jvt, 3rsd, 1xpt, 1rbb, 2blz, 3rsp, 1ssc, 8rat, 2apq, 1j7z, 1w4o, 1rbd, 1rnz, 1jvv, 1rar, 1kf3, 1z3l, 1aqp, 1rbh, 2w5m, 1rbg, 1o0n, 1js0, 3rsk, 1ssa, 1cjr, 1eic, 1d5d, 1ymn, 1j81, 1rnw, 2w5k, 1rph, 2rns, 1rta, 1rnu, 1rny, 1z3p, 1gv7, 1rcn, 1rnv, 4rat, 1w4p, 4rsd, 1a5p, 1wbu, 1rha, 1ras, 2blp, 1rnd, 1kf2, 1d5h, 1srn, 1d5e, 2rat, 1izp, 2w5g, 1o0f, 1izr, 1c9x, 2rln, 4srn, 1c9v, 1u1b, 5rat, 1ruv, 1rbc, 2w5i, 1afl, 1z6d, 1rbe, 1a5q, 1kh8, 1lsq, 1jvu, 1a2w, 3rn3, 3rat
Activity:	Pancreatic ribonuclease, with EC number 3.1.27.5
Resources:	FirstGlance, OCA, RCSB, PDBsum

Function

[RNAS1_BOVIN] Endonuclease that catalyzes the cleavage of RNA on the 3' side of pyrimidine nucleotides. Acts on single stranded and double stranded RNA.^[1]

Publication Abstract from PubMed

Molecular probes are useful for both studying and controlling the functions of enzymes and other proteins. The most useful probes have high affinity for their target, along with small size and resistance to degradation. Here, we report on new surrogates for nucleic acids that fulfill these criteria. Isosteres in which phosphoryl [R-O-P(O(2) (-) )-O-R'] groups are replaced with N-acylsulfonamidyl [R-C(O)-N(-) -S(O(2) )-R'] or sulfonimidyl [R-S(O(2) )-N(-) -S(O(2) )-R'] groups increase the number of nonbridging oxygens from two (phosphoryl) to three (N-acylsulfonamidyl) or four (sulfonimidyl). Six such isosteres were found to be more potent inhibitors of catalysis by bovine pancreatic RNase A than are parent compounds containing phosphoryl groups. The atomic structures of two RNase A.N-acylsulfonamide complexes were determined at high resolution by X-ray crystallography. The N-acylsulfonamidyl groups were observed to form more hydrogen bonds with active site residues than did the phosphoryl groups in analogous complexes. These data encourage the further development and use of N-acylsulfonamides and sulfonimides as antagonists of nucleic acid-binding proteins.

Functional and structural analyses of N-acylsulfonamide-linked dinucleoside inhibitors of RNase A.,Thiyagarajan N, Smith BD, Raines RT, Acharya KR FEBS J. 2010 Dec 4. doi: 10.1111/j.1742-4658.2010.07976.x. PMID:21205197^[2]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ delCardayre SB, Ribo M, Yokel EM, Quirk DJ, Rutter WJ, Raines RT. Engineering ribonuclease A: production, purification and characterization of wild-type enzyme and mutants at Gln11. Protein Eng. 1995 Mar;8(3):261-73. PMID:7479688
↑ Thiyagarajan N, Smith BD, Raines RT, Acharya KR. Functional and structural analyses of N-acylsulfonamide-linked dinucleoside inhibitors of RNase A. FEBS J. 2010 Dec 4. doi: 10.1111/j.1742-4658.2010.07976.x. PMID:21205197 doi:10.1111/j.1742-4658.2010.07976.x

1 Structural highlights
2 Function
3 Publication Abstract from PubMed
4 See Also
5 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/2xog"

@@ Line 8: / Line 8: @@
 <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2xog FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2xog OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2xog RCSB], [http://www.ebi.ac.uk/pdbsum/2xog PDBsum]</span></td></tr>
 </table>
+== Function ==
+[[http://www.uniprot.org/uniprot/RNAS1_BOVIN RNAS1_BOVIN]] Endonuclease that catalyzes the cleavage of RNA on the 3' side of pyrimidine nucleotides. Acts on single stranded and double stranded RNA.<ref>PMID:7479688</ref>
 <div style="background-color:#fffaf0;">
 == Publication Abstract from PubMed ==
@@ Line 19: / Line 21: @@
 ==See Also==
 *[[Ribonuclease|Ribonuclease]]
+*[[Temp|Temp]]
+*[[User:Jaime.Prilusky/Test/tree|User:Jaime.Prilusky/Test/tree]]
 == References ==
 <references/>
@@ Line 25: / Line 29: @@
 [[Category: Bos taurus]]
 [[Category: Pancreatic ribonuclease]]
-[[Category: Acharya, K R.]]
+[[Category: Acharya, K R]]
-[[Category: Raines, R T.]]
+[[Category: Raines, R T]]
-[[Category: Smith, B D.]]
+[[Category: Smith, B D]]
-[[Category: Thiyagarajan, N.]]
+[[Category: Thiyagarajan, N]]
 [[Category: Hydrolase]]

2xog

From Proteopedia

Revision as of 09:47, 25 December 2014

FUNCTIONAL AND STRUCTURAL ANALYSES OF N-ACYLSULFONAMIDE-LINKED DINUCLEOSIDE INHIBITORS OF RIBONUCLEASE A

Structural highlights

Function

Publication Abstract from PubMed

See Also

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

Views

Personal tools

Navigation

Search

Toolbox