2yn0
From Proteopedia
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| - | + | ==tau55 histidine phosphatase domain== | |
| - | === | + | <StructureSection load='2yn0' size='340' side='right' caption='[[2yn0]], [[Resolution|resolution]] 1.50Å' scene=''> |
| - | + | == Structural highlights == | |
| - | + | <table><tr><td colspan='2'>[[2yn0]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae_s288c Saccharomyces cerevisiae s288c]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2YN0 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2YN0 FirstGlance]. <br> | |
| - | ==Function== | + | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene></td></tr> |
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2yn0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2yn0 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2yn0 RCSB], [http://www.ebi.ac.uk/pdbsum/2yn0 PDBsum]</span></td></tr> | ||
| + | </table> | ||
| + | == Function == | ||
[[http://www.uniprot.org/uniprot/TFC7_YEAST TFC7_YEAST]] TFIIIC mediates tRNA and 5S RNA gene activation by binding to intragenic promoter elements. Upstream of the transcription start site, TFIIIC assembles the initiation complex TFIIIB-TFIIIC-tDNA, which is sufficient for RNA polymerase III recruitment and function. Part of the tauA domain of TFIIIC that binds boxA DNA promoter sites of tRNA and similar genes.<ref>PMID:9584160</ref> | [[http://www.uniprot.org/uniprot/TFC7_YEAST TFC7_YEAST]] TFIIIC mediates tRNA and 5S RNA gene activation by binding to intragenic promoter elements. Upstream of the transcription start site, TFIIIC assembles the initiation complex TFIIIB-TFIIIC-tDNA, which is sufficient for RNA polymerase III recruitment and function. Part of the tauA domain of TFIIIC that binds boxA DNA promoter sites of tRNA and similar genes.<ref>PMID:9584160</ref> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | Saccharomyces cerevisiae tau55, a subunit of the RNA polymerase III-specific general transcription factor TFIIIC, comprises an N-terminal histidine phosphatase domain (tau55-HPD) whose catalytic activity and cellular function is poorly understood. We solved the crystal structures of tau55-HPD and its closely related paralogue Huf and used in silico docking methods to identify phospho-serine and phospho-tyrosine containing peptides as possible substrates that were subsequently validated using in vitro phosphatase assays. A comparative phospho-proteomic study identified additional phosphopeptides as possible targets, which show the involvement of these two phosphatases in the regulation of a variety of cellular functions. Our results identify tau55-HPD and Huf as bona fide protein phosphatases, characterize their substrate specificities and provide a small set of regulated phosphosite targets in vivo. | ||
| - | + | Structural and functional characterization of a phosphatase domain within yeast general transcription factor TFIIIC.,Taylor NM, Glatt S, Hennrich ML, von Scheven G, Grotsch H, Fernandez-Tornero C, Rybin V, Gavin AC, Kolb P, Muller CW J Biol Chem. 2013 Apr 8. PMID:23569204<ref>PMID:23569204</ref> | |
| - | + | ||
| - | == | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> |
| - | + | </div> | |
| + | == References == | ||
| + | <references/> | ||
| + | __TOC__ | ||
| + | </StructureSection> | ||
[[Category: Saccharomyces cerevisiae s288c]] | [[Category: Saccharomyces cerevisiae s288c]] | ||
| - | [[Category: Fernandez-Tornero, C | + | [[Category: Fernandez-Tornero, C]] |
| - | [[Category: Gavin, A C | + | [[Category: Gavin, A C]] |
| - | [[Category: Glatt, S | + | [[Category: Glatt, S]] |
| - | [[Category: Grotsch, H | + | [[Category: Grotsch, H]] |
| - | [[Category: Hennrich, M | + | [[Category: Hennrich, M]] |
| - | [[Category: Kolb, P | + | [[Category: Kolb, P]] |
| - | [[Category: Muller, C W | + | [[Category: Muller, C W]] |
| - | [[Category: Rybin, V | + | [[Category: Rybin, V]] |
| - | [[Category: Scheven, G von | + | [[Category: Scheven, G von]] |
| - | [[Category: Taylor, N M.I | + | [[Category: Taylor, N M.I]] |
[[Category: Rna polymerase iii]] | [[Category: Rna polymerase iii]] | ||
[[Category: Transcription]] | [[Category: Transcription]] | ||
Revision as of 09:50, 25 December 2014
tau55 histidine phosphatase domain
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