2crx
From Proteopedia
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| - | [[Image:2crx.gif|left|200px]] | + | [[Image:2crx.gif|left|200px]] |
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| - | '''STRUCTURE OF THE HOLLIDAY JUNCTION INTERMEDIATE IN CRE-LOXP SITE-SPECIFIC RECOMBINATION''' | + | {{Structure |
| + | |PDB= 2crx |SIZE=350|CAPTION= <scene name='initialview01'>2crx</scene>, resolution 2.5Å | ||
| + | |SITE= <scene name='pdbsite=CIA:Recombinase+Active+Site'>CIA</scene> and <scene name='pdbsite=CIB:Recombinase+Active+Site'>CIB</scene> | ||
| + | |LIGAND= | ||
| + | |ACTIVITY= | ||
| + | |GENE= | ||
| + | }} | ||
| + | |||
| + | '''STRUCTURE OF THE HOLLIDAY JUNCTION INTERMEDIATE IN CRE-LOXP SITE-SPECIFIC RECOMBINATION''' | ||
| + | |||
==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
| - | 2CRX is a [ | + | 2CRX is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Bacteriophage_p1 Bacteriophage p1]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2CRX OCA]. |
==Reference== | ==Reference== | ||
| - | Structure of the Holliday junction intermediate in Cre-loxP site-specific recombination., Gopaul DN, Guo F, Van Duyne GD, EMBO J. 1998 Jul 15;17(14):4175-87. PMID:[http:// | + | Structure of the Holliday junction intermediate in Cre-loxP site-specific recombination., Gopaul DN, Guo F, Van Duyne GD, EMBO J. 1998 Jul 15;17(14):4175-87. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/9670032 9670032] |
[[Category: Bacteriophage p1]] | [[Category: Bacteriophage p1]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
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[[Category: recombination]] | [[Category: recombination]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 16:19:12 2008'' |
Revision as of 14:19, 20 March 2008
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| , resolution 2.5Å | |||||||
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| Coordinates: | save as pdb, mmCIF, xml | ||||||
STRUCTURE OF THE HOLLIDAY JUNCTION INTERMEDIATE IN CRE-LOXP SITE-SPECIFIC RECOMBINATION
Overview
We have determined the X-ray crystal structures of two DNA Holliday junctions (HJs) bound by Cre recombinase. The HJ is a four-way branched structure that occurs as an intermediate in genetic recombination pathways, including site-specific recombination by the lambda-integrase family. Cre recombinase is an integrase family member that recombines 34 bp loxP sites in the absence of accessory proteins or auxiliary DNA sequences. The 2.7 A structure of Cre recombinase bound to an immobile HJ and the 2.5 A structure of Cre recombinase bound to a symmetric, nicked HJ reveal a nearly planar, twofold-symmetric DNA intermediate that shares features with both the stacked-X and the square conformations of the HJ that exist in the unbound state. The structures support a protein-mediated crossover isomerization of the junction that acts as the switch responsible for activation and deactivation of recombinase active sites. In this model, a subtle isomerization of the Cre recombinase-HJ quaternary structure dictates which strands are cleaved during resolution of the junction via a mechanism that involves neither branch migration nor helical restacking.
About this Structure
2CRX is a Single protein structure of sequence from Bacteriophage p1. Full crystallographic information is available from OCA.
Reference
Structure of the Holliday junction intermediate in Cre-loxP site-specific recombination., Gopaul DN, Guo F, Van Duyne GD, EMBO J. 1998 Jul 15;17(14):4175-87. PMID:9670032
Page seeded by OCA on Thu Mar 20 16:19:12 2008
