2d1e
From Proteopedia
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| - | [[Image:2d1e.gif|left|200px]] | + | [[Image:2d1e.gif|left|200px]] |
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| - | '''Crystal structure of PcyA-biliverdin complex''' | + | {{Structure |
| + | |PDB= 2d1e |SIZE=350|CAPTION= <scene name='initialview01'>2d1e</scene>, resolution 1.510Å | ||
| + | |SITE= | ||
| + | |LIGAND= <scene name='pdbligand=NA:SODIUM+ION'>NA</scene> and <scene name='pdbligand=BLA:BILIVERDINE IX ALPHA'>BLA</scene> | ||
| + | |ACTIVITY= [http://en.wikipedia.org/wiki/Phycocyanobilin:ferredoxin_oxidoreductase Phycocyanobilin:ferredoxin oxidoreductase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.3.7.5 1.3.7.5] | ||
| + | |GENE= | ||
| + | }} | ||
| + | |||
| + | '''Crystal structure of PcyA-biliverdin complex''' | ||
| + | |||
==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
| - | 2D1E is a [ | + | 2D1E is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Synechocystis_sp. Synechocystis sp.]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2D1E OCA]. |
==Reference== | ==Reference== | ||
| - | Crystal structure of phycocyanobilin:ferredoxin oxidoreductase in complex with biliverdin IXalpha, a key enzyme in the biosynthesis of phycocyanobilin., Hagiwara Y, Sugishima M, Takahashi Y, Fukuyama K, Proc Natl Acad Sci U S A. 2006 Jan 3;103(1):27-32. Epub 2005 Dec 27. PMID:[http:// | + | Crystal structure of phycocyanobilin:ferredoxin oxidoreductase in complex with biliverdin IXalpha, a key enzyme in the biosynthesis of phycocyanobilin., Hagiwara Y, Sugishima M, Takahashi Y, Fukuyama K, Proc Natl Acad Sci U S A. 2006 Jan 3;103(1):27-32. Epub 2005 Dec 27. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/16380422 16380422] |
[[Category: Phycocyanobilin:ferredoxin oxidoreductase]] | [[Category: Phycocyanobilin:ferredoxin oxidoreductase]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
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[[Category: enzyme-substrate complex]] | [[Category: enzyme-substrate complex]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 16:22:12 2008'' |
Revision as of 14:22, 20 March 2008
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| , resolution 1.510Å | |||||||
|---|---|---|---|---|---|---|---|
| Ligands: | and | ||||||
| Activity: | Phycocyanobilin:ferredoxin oxidoreductase, with EC number 1.3.7.5 | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
Crystal structure of PcyA-biliverdin complex
Overview
Phytobilins (light harvesting and photoreceptor pigments in higher plants, algae, and cyanobacteria) are synthesized from biliverdin IXalpha (BV) by ferredoxin-dependent bilin reductases (FDBRs). Phycocyanobilin:ferredoxin oxidoreductase (PcyA), one such FDBR, is a new class of radical enzymes that require neither cofactors nor metals and serially reduces the vinyl group of the D-ring and A-ring of BV using four electrons from ferredoxin to produce phycocyanobilin, one of the phytobilins. We have determined the crystal structure of PcyA from Synechocystis sp. PCC 6803 in complex with BV, revealing the first tertiary structure of an FDBR family member. PcyA is folded in a three-layer alpha/beta/alpha sandwich structure, in which BV in a cyclic conformation is positioned between the beta-sheet and C-terminal alpha-helices. The basic patch on the PcyA surface near the BV molecule may provide a binding site for acidic ferredoxin, allowing direct transfer of electrons to BV. The orientation of BV is definitely fixed in PcyA by several hydrophilic interactions and the shape of the BV binding pocket of PcyA. We propose the mechanism by which the sequential reduction of the D- and A-rings is controlled, where Asp-105, located between the two reduction sites, would play the central role by changing its conformation during the reaction. Homology modeling of other FDBRs based on the PcyA structure fits well with previous genetic and biochemical data, thereby providing a structural basis for the reaction mechanism of FDBRs.
About this Structure
2D1E is a Single protein structure of sequence from Synechocystis sp.. Full crystallographic information is available from OCA.
Reference
Crystal structure of phycocyanobilin:ferredoxin oxidoreductase in complex with biliverdin IXalpha, a key enzyme in the biosynthesis of phycocyanobilin., Hagiwara Y, Sugishima M, Takahashi Y, Fukuyama K, Proc Natl Acad Sci U S A. 2006 Jan 3;103(1):27-32. Epub 2005 Dec 27. PMID:16380422
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