4e2i
From Proteopedia
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| - | + | ==The Complex Structure of the SV40 Helicase Large T Antigen and p68 Subunit of DNA Polymerase Alpha-Primase== | |
| - | + | <StructureSection load='4e2i' size='340' side='right' caption='[[4e2i]], [[Resolution|resolution]] 5.00Å' scene=''> | |
| - | + | == Structural highlights == | |
| - | + | <table><tr><td colspan='2'>[[4e2i]] is a 23 chain structure with sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] and [http://en.wikipedia.org/wiki/Simian_virus_40 Simian virus 40]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4E2I OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4E2I FirstGlance]. <br> | |
| - | ==Function== | + | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr> |
| + | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">POLA2 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 Homo sapiens])</td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4e2i FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4e2i OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4e2i RCSB], [http://www.ebi.ac.uk/pdbsum/4e2i PDBsum]</span></td></tr> | ||
| + | </table> | ||
| + | == Function == | ||
[[http://www.uniprot.org/uniprot/DPOA2_HUMAN DPOA2_HUMAN]] May play an essential role at the early stage of chromosomal DNA replication by coupling the polymerase alpha/primase complex to the cellular replication machinery (By similarity). | [[http://www.uniprot.org/uniprot/DPOA2_HUMAN DPOA2_HUMAN]] May play an essential role at the early stage of chromosomal DNA replication by coupling the polymerase alpha/primase complex to the cellular replication machinery (By similarity). | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | DNA polymerase alpha-primase (Pol-prim) plays an essential role in eukaryotic DNA replication, initiating synthesis of the leading strand and of each Okazaki fragment on the lagging strand. Pol-prim is composed of a primase heterodimer that synthesizes an RNA primer, a DNA polymerase subunit that extends the primer, and a regulatory B-subunit (p68) without apparent enzymatic activity. Pol-prim is thought to interact with eukaryotic replicative helicases, forming a dynamic multiprotein assembly that displays primosome activity. At least three subunits of Pol-prim interact physically with the hexameric replicative helicase SV40 large T antigen, constituting a simple primosome that is active in vitro. However, structural understanding of these interactions and their role in viral chromatin replication in vivo remains incomplete. Here, we report the detailed large T antigen-p68 interface, as revealed in a co-crystal structure and validated by site-directed mutagenesis, and we demonstrate its functional importance in activating the SV40 primosome in cell-free reactions with purified Pol-prim, as well as in monkey cells in vivo. | ||
| - | + | Structural basis for the interaction of a hexameric replicative helicase with the regulatory subunit of human DNA polymerase alpha-primase.,Zhou B, Arnett DR, Yu X, Brewster A, Sowd GA, Xie CL, Vila S, Gai D, Fanning E, Chen XS J Biol Chem. 2012 Aug 3;287(32):26854-66. doi: 10.1074/jbc.M112.363655. Epub 2012, Jun 14. PMID:22700977<ref>PMID:22700977</ref> | |
| - | + | ||
| + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | ||
| + | </div> | ||
==See Also== | ==See Also== | ||
*[[DNA polymerase|DNA polymerase]] | *[[DNA polymerase|DNA polymerase]] | ||
| - | + | *[[Large T Antigen|Large T Antigen]] | |
| - | == | + | == References == |
| - | + | <references/> | |
| + | __TOC__ | ||
| + | </StructureSection> | ||
[[Category: Homo sapiens]] | [[Category: Homo sapiens]] | ||
[[Category: Simian virus 40]] | [[Category: Simian virus 40]] | ||
| - | [[Category: Arnett, D R | + | [[Category: Arnett, D R]] |
| - | [[Category: Brewster, A | + | [[Category: Brewster, A]] |
| - | [[Category: Chen, X S | + | [[Category: Chen, X S]] |
| - | [[Category: Fanning, E | + | [[Category: Fanning, E]] |
| - | [[Category: Gai, D | + | [[Category: Gai, D]] |
| - | [[Category: Sowd, G A | + | [[Category: Sowd, G A]] |
| - | [[Category: Vila, S | + | [[Category: Vila, S]] |
| - | [[Category: Xie, C L | + | [[Category: Xie, C L]] |
| - | [[Category: Yu, X | + | [[Category: Yu, X]] |
| - | [[Category: Zhou, B | + | [[Category: Zhou, B]] |
[[Category: Hydrolase-dna binding complex]] | [[Category: Hydrolase-dna binding complex]] | ||
[[Category: Hydrolase-dna binding protein complex]] | [[Category: Hydrolase-dna binding protein complex]] | ||
[[Category: Replication initiation]] | [[Category: Replication initiation]] | ||
Revision as of 11:41, 25 December 2014
The Complex Structure of the SV40 Helicase Large T Antigen and p68 Subunit of DNA Polymerase Alpha-Primase
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