This old version of Proteopedia is provided for student assignments while the new version is undergoing repairs. Content and edits done in this old version of Proteopedia after March 1, 2026 will eventually be lost when it is retired in about June of 2026.
Apply for new accounts at the new Proteopedia. Your logins will work in both the old and new versions.
4e2i
From Proteopedia
(Difference between revisions)
| Line 1: | Line 1: | ||
| - | + | ==The Complex Structure of the SV40 Helicase Large T Antigen and p68 Subunit of DNA Polymerase Alpha-Primase== | |
| - | + | <StructureSection load='4e2i' size='340' side='right' caption='[[4e2i]], [[Resolution|resolution]] 5.00Å' scene=''> | |
| - | + | == Structural highlights == | |
| - | + | <table><tr><td colspan='2'>[[4e2i]] is a 23 chain structure with sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] and [http://en.wikipedia.org/wiki/Simian_virus_40 Simian virus 40]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4E2I OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4E2I FirstGlance]. <br> | |
| - | ==Function== | + | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr> |
| + | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">POLA2 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 Homo sapiens])</td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4e2i FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4e2i OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4e2i RCSB], [http://www.ebi.ac.uk/pdbsum/4e2i PDBsum]</span></td></tr> | ||
| + | </table> | ||
| + | == Function == | ||
[[http://www.uniprot.org/uniprot/DPOA2_HUMAN DPOA2_HUMAN]] May play an essential role at the early stage of chromosomal DNA replication by coupling the polymerase alpha/primase complex to the cellular replication machinery (By similarity). | [[http://www.uniprot.org/uniprot/DPOA2_HUMAN DPOA2_HUMAN]] May play an essential role at the early stage of chromosomal DNA replication by coupling the polymerase alpha/primase complex to the cellular replication machinery (By similarity). | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | DNA polymerase alpha-primase (Pol-prim) plays an essential role in eukaryotic DNA replication, initiating synthesis of the leading strand and of each Okazaki fragment on the lagging strand. Pol-prim is composed of a primase heterodimer that synthesizes an RNA primer, a DNA polymerase subunit that extends the primer, and a regulatory B-subunit (p68) without apparent enzymatic activity. Pol-prim is thought to interact with eukaryotic replicative helicases, forming a dynamic multiprotein assembly that displays primosome activity. At least three subunits of Pol-prim interact physically with the hexameric replicative helicase SV40 large T antigen, constituting a simple primosome that is active in vitro. However, structural understanding of these interactions and their role in viral chromatin replication in vivo remains incomplete. Here, we report the detailed large T antigen-p68 interface, as revealed in a co-crystal structure and validated by site-directed mutagenesis, and we demonstrate its functional importance in activating the SV40 primosome in cell-free reactions with purified Pol-prim, as well as in monkey cells in vivo. | ||
| - | + | Structural basis for the interaction of a hexameric replicative helicase with the regulatory subunit of human DNA polymerase alpha-primase.,Zhou B, Arnett DR, Yu X, Brewster A, Sowd GA, Xie CL, Vila S, Gai D, Fanning E, Chen XS J Biol Chem. 2012 Aug 3;287(32):26854-66. doi: 10.1074/jbc.M112.363655. Epub 2012, Jun 14. PMID:22700977<ref>PMID:22700977</ref> | |
| - | + | ||
| + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | ||
| + | </div> | ||
==See Also== | ==See Also== | ||
*[[DNA polymerase|DNA polymerase]] | *[[DNA polymerase|DNA polymerase]] | ||
| - | + | *[[Large T Antigen|Large T Antigen]] | |
| - | == | + | == References == |
| - | + | <references/> | |
| + | __TOC__ | ||
| + | </StructureSection> | ||
[[Category: Homo sapiens]] | [[Category: Homo sapiens]] | ||
[[Category: Simian virus 40]] | [[Category: Simian virus 40]] | ||
| - | [[Category: Arnett, D R | + | [[Category: Arnett, D R]] |
| - | [[Category: Brewster, A | + | [[Category: Brewster, A]] |
| - | [[Category: Chen, X S | + | [[Category: Chen, X S]] |
| - | [[Category: Fanning, E | + | [[Category: Fanning, E]] |
| - | [[Category: Gai, D | + | [[Category: Gai, D]] |
| - | [[Category: Sowd, G A | + | [[Category: Sowd, G A]] |
| - | [[Category: Vila, S | + | [[Category: Vila, S]] |
| - | [[Category: Xie, C L | + | [[Category: Xie, C L]] |
| - | [[Category: Yu, X | + | [[Category: Yu, X]] |
| - | [[Category: Zhou, B | + | [[Category: Zhou, B]] |
[[Category: Hydrolase-dna binding complex]] | [[Category: Hydrolase-dna binding complex]] | ||
[[Category: Hydrolase-dna binding protein complex]] | [[Category: Hydrolase-dna binding protein complex]] | ||
[[Category: Replication initiation]] | [[Category: Replication initiation]] | ||
Revision as of 11:41, 25 December 2014
The Complex Structure of the SV40 Helicase Large T Antigen and p68 Subunit of DNA Polymerase Alpha-Primase
| |||||||||||
