This old version of Proteopedia is provided for student assignments while the new version is undergoing repairs. Content and edits done in this old version of Proteopedia after March 1, 2026 will eventually be lost when it is retired in about June of 2026.

Apply for new accounts at the new Proteopedia. Your logins will work in both the old and new versions.

4a71

From Proteopedia

(Difference between revisions)

Jump to: navigation, search

Revision as of 12:18, 25 December 2014

cytochrome c peroxidase in complex with phenol

Structural highlights

4a71 is a 1 chain structure with sequence from Saccharomyces cerevisiae. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Ligands:	,
Related:	1cpg, 1bva, 2v23, 3ccx, 6ccp, 1aeu, 1dcc, 1s6v, 1bej, 1ml2, 1ccl, 1beq, 1aem, 1jci, 1u74, 2pcc, 2b10, 1aeb, 1cpd, 1ccg, 1cca, 1ac4, 2y5a, 1aeo, 1cmu, 1aet, 1bep, 1aeh, 1aev, 2pcb, 1kxn, 1aeg, 1aen, 4ccp, 5ccp, 2x07, 2cep, 1cmp, 1ryc, 1cce, 2xj8, 2b0z, 1dsp, 1bes, 1z53, 2b12, 1bem, 1mkr, 1ccj, 1sog, 1aee, 1cpe, 1a2f, 1dse, 1jdr, 1a2g, 1aes, 1bek, 1zbz, 1cck, 1mkq, 1mk8, 1ebe, 1cci, 1krj, 2b11, 1aef, 1cyf, 1aek, 1ccb, 1u75, 1ac8, 1dsg, 1dj1, 1dj5, 2ccp, 1cmt, 1aej, 7ccp, 1aeq, 1ds4, 2x08, 1sdq, 2gb8, 2xj5, 1kxm, 4ccx, 1kok, 1bj9, 2bcn, 1zby, 1ccp, 2ycg, 2cyp, 1dso, 3ccp, 1stq, 2v2e, 2xil, 1aa4, 1aed, 1ccc, 1cmq, 1cpf, 4a6z
Activity:	Cytochrome-c peroxidase, with EC number 1.11.1.5
Resources:	FirstGlance, OCA, RCSB, PDBsum

Function

[CCPR_YEAST] Destroys radicals which are normally produced within the cells and which are toxic to biological systems.

Publication Abstract from PubMed

Guaiacol is a universal substrate for all peroxidases, and its use in a simple colorimetric assay has wide applications. However, its exact binding location has never been defined. Here we report the crystal structures of guaiacol bound to cytochrome c peroxidase (CcP). A related structure with phenol bound is also presented. The CcP-guaiacol and CcP-phenol crystal structures show that both guaiacol and phenol bind at sites distinct from the cytochrome c binding site and from the delta-heme edge, which is known to be the binding site for other substrates. Although neither guaiacol nor phenol is seen bound at the delta-heme edge in the crystal structures, inhibition data and mutagenesis strongly suggest that the catalytic binding site for aromatic compounds is the delta-heme edge in CcP. The functional implications of these observations are discussed in terms of our existing understanding of substrate binding in peroxidases [Gumiero A et al. (2010) Arch Biochem Biophys 500, 13-20].

Crystal structure of guaiacol and phenol bound to a heme peroxidase.,Murphy EJ, Metcalfe CL, Nnamchi C, Moody PC, Raven EL FEBS J. 2012 May;279(9):1632-9. doi: 10.1111/j.1742-4658.2011.08425.x. Epub 2011 , Dec 5. PMID:22093282^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Murphy EJ, Metcalfe CL, Nnamchi C, Moody PC, Raven EL. Crystal structure of guaiacol and phenol bound to a heme peroxidase. FEBS J. 2012 May;279(9):1632-9. doi: 10.1111/j.1742-4658.2011.08425.x. Epub 2011 , Dec 5. PMID:22093282 doi:http://dx.doi.org/10.1111/j.1742-4658.2011.08425.x

1 Structural highlights
2 Function
3 Publication Abstract from PubMed
4 See Also
5 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/4a71"

@@ Line 8: / Line 8: @@
 <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4a71 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4a71 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4a71 RCSB], [http://www.ebi.ac.uk/pdbsum/4a71 PDBsum]</span></td></tr>
 </table>
+== Function ==
+[[http://www.uniprot.org/uniprot/CCPR_YEAST CCPR_YEAST]] Destroys radicals which are normally produced within the cells and which are toxic to biological systems.
 <div style="background-color:#fffaf0;">
 == Publication Abstract from PubMed ==

4a71

From Proteopedia

Revision as of 12:18, 25 December 2014

cytochrome c peroxidase in complex with phenol

Structural highlights

Function

Publication Abstract from PubMed

See Also

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

Views

Personal tools

Navigation

Search

Toolbox