4o8x

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{{STRUCTURE_4o8x| PDB=4o8x | SCENE= }}
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==Zinc-bound Rpn11 in complex with Rpn8==
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===Zinc-bound Rpn11 in complex with Rpn8===
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<StructureSection load='4o8x' size='340' side='right' caption='[[4o8x]], [[Resolution|resolution]] 1.99&Aring;' scene=''>
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{{ABSTRACT_PUBMED_24463465}}
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== Structural highlights ==
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<table><tr><td colspan='2'>[[4o8x]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Baker's_yeast Baker's yeast]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4O8X OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4O8X FirstGlance]. <br>
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==Function==
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</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
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<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[4o8y|4o8y]]</td></tr>
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<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">RPN8, YOR261C, O5360 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=559292 Baker's yeast]), RPN11, MPR1, YFR004W ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=559292 Baker's yeast])</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4o8x FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4o8x OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4o8x RCSB], [http://www.ebi.ac.uk/pdbsum/4o8x PDBsum]</span></td></tr>
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</table>
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== Function ==
[[http://www.uniprot.org/uniprot/RPN8_YEAST RPN8_YEAST]] Acts as a regulatory subunit of the 26S proteasome which is involved in the ATP-dependent degradation of ubiquitinated proteins.<ref>PMID:9584156</ref> [[http://www.uniprot.org/uniprot/RPN11_YEAST RPN11_YEAST]] Acts as a regulatory subunit of the 26 proteasome which is involved in the ATP-dependent degradation of ubiquitinated proteins.<ref>PMID:21075847</ref>
[[http://www.uniprot.org/uniprot/RPN8_YEAST RPN8_YEAST]] Acts as a regulatory subunit of the 26S proteasome which is involved in the ATP-dependent degradation of ubiquitinated proteins.<ref>PMID:9584156</ref> [[http://www.uniprot.org/uniprot/RPN11_YEAST RPN11_YEAST]] Acts as a regulatory subunit of the 26 proteasome which is involved in the ATP-dependent degradation of ubiquitinated proteins.<ref>PMID:21075847</ref>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Polyubiquitin chains target protein substrates to the 26S proteasome, where they are removed by the deubiquitinase Rpn11 to allow efficient substrate degradation. Despite Rpn11's essential function during substrate processing, its detailed structural and biochemical characterization has been hindered by difficulties in purifying the isolated enzyme. Here we report the 2.0-A crystal structures of Zn2+-free and Zn2+-bound Saccharomyces cerevisiae Rpn11 in an MPN-domain heterodimer with Rpn8. The Rpn11-Rpn8 interaction occurs via two distinct interfaces that may be conserved in related MPN-domain complexes. Our structural and mutational studies reveal that Rpn11 lacks a conserved surface to bind the ubiquitin Ile44 patch, does not interact with the moiety on the proximal side of the scissile isopeptide bond and exhibits no linkage specificity for ubiquitin cleavage. These findings explain how Rpn11 functions as a promiscuous deubiquitinase for cotranslocational substrate deubiquitination during proteasomal degradation.
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Structure of the Rpn11-Rpn8 dimer reveals mechanisms of substrate deubiquitination during proteasomal degradation.,Worden EJ, Padovani C, Martin A Nat Struct Mol Biol. 2014 Jan 23. doi: 10.1038/nsmb.2771. PMID:24463465<ref>PMID:24463465</ref>
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==About this Structure==
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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[[4o8x]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4O8X OCA].
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</div>
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==Reference==
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==See Also==
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<ref group="xtra">PMID:024463465</ref><references group="xtra"/><references/>
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*[[Proteasome|Proteasome]]
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[[Category: Martin, A.]]
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== References ==
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[[Category: Padovani, C.]]
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<references/>
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[[Category: Worden, E J.]]
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__TOC__
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</StructureSection>
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[[Category: Baker's yeast]]
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[[Category: Martin, A]]
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[[Category: Padovani, C]]
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[[Category: Worden, E J]]
[[Category: Deubiquitinase]]
[[Category: Deubiquitinase]]
[[Category: Hydrolase]]
[[Category: Hydrolase]]
[[Category: Jamm]]
[[Category: Jamm]]
[[Category: Mpn]]
[[Category: Mpn]]

Revision as of 12:27, 25 December 2014

Zinc-bound Rpn11 in complex with Rpn8

4o8x, resolution 1.99Å

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