3fyh

From Proteopedia

(Difference between revisions)

Revision as of 12:28, 25 December 2014

Recombinase in complex with ADP and metatungstate

Structural highlights

3fyh is a 1 chain structure with sequence from Methanococcus voltae. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Ligands:	, , ,
Related:	2f1j
Gene:	radA (Methanococcus voltae)
Resources:	FirstGlance, OCA, RCSB, PDBsum

Function

[RADA_METVO] Involved in DNA repair and in homologous recombination. Binds and assemble on single-stranded DNA to form a nucleoprotein filament. Hydrolyzes ATP in a ssDNA-dependent manner and promotes DNA strand exchange between homologous DNA molecules (By similarity).

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

Publication Abstract from PubMed

Archaeal RadAs are close homologues of eukaryal Rad51s ( approximately 40% sequence identities). These recombinases promote a hallmark strand exchange process between homologous single-stranded and double-stranded DNA substrates. This DNA-repairing function also plays a key role in cancer cells' resistance to chemo- and radiotherapy. Inhibition of the strand exchange process may render cancer cells more susceptible to therapeutic treatment. We found that metatungstate is a potent inhibitor of RadA from Methanococcus voltae. The tungsten cluster binds RadA in the axial DNA-binding groove. This polyanionic species appears to inhibit RadA by locking the protein in its inactive conformation.

Crystal structure of an archaeal Rad51 homologue in complex with a metatungstate inhibitor.,Li Y, He Y, Luo Y Biochemistry. 2009 Jul 28;48(29):6805-10. PMID:19555119^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Li Y, He Y, Luo Y. Crystal structure of an archaeal Rad51 homologue in complex with a metatungstate inhibitor. Biochemistry. 2009 Jul 28;48(29):6805-10. PMID:19555119 doi:10.1021/bi900832t

1 Structural highlights
2 Function
3 Evolutionary Conservation
4 Publication Abstract from PubMed
5 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/3fyh"

@@ Line 8: / Line 8: @@
 <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3fyh FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3fyh OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3fyh RCSB], [http://www.ebi.ac.uk/pdbsum/3fyh PDBsum]</span></td></tr>
 </table>
+== Function ==
+[[http://www.uniprot.org/uniprot/RADA_METVO RADA_METVO]] Involved in DNA repair and in homologous recombination. Binds and assemble on single-stranded DNA to form a nucleoprotein filament. Hydrolyzes ATP in a ssDNA-dependent manner and promotes DNA strand exchange between homologous DNA molecules (By similarity).
 == Evolutionary Conservation ==
 [[Image:Consurf_key_small.gif|200px|right]]

3fyh

From Proteopedia

Revision as of 12:28, 25 December 2014

Recombinase in complex with ADP and metatungstate

Structural highlights

Function

Evolutionary Conservation

Publication Abstract from PubMed

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

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