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2dfw
From Proteopedia
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| - | [[Image:2dfw.gif|left|200px]] | + | [[Image:2dfw.gif|left|200px]] |
| - | + | ||
| - | '''Crystal structure of a major fragment of the salt-tolerant glutaminase from Micrococcus luteus K-3''' | + | {{Structure |
| + | |PDB= 2dfw |SIZE=350|CAPTION= <scene name='initialview01'>2dfw</scene>, resolution 2.44Å | ||
| + | |SITE= | ||
| + | |LIGAND= | ||
| + | |ACTIVITY= [http://en.wikipedia.org/wiki/Glutaminase Glutaminase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.5.1.2 3.5.1.2] | ||
| + | |GENE= | ||
| + | }} | ||
| + | |||
| + | '''Crystal structure of a major fragment of the salt-tolerant glutaminase from Micrococcus luteus K-3''' | ||
| + | |||
==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
| - | 2DFW is a [ | + | 2DFW is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Micrococcus_luteus Micrococcus luteus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2DFW OCA]. |
==Reference== | ==Reference== | ||
| - | Crystal structure of a major fragment of the salt-tolerant glutaminase from Micrococcus luteus K-3., Yoshimune K, Shirakihara Y, Shiratori A, Wakayama M, Chantawannakul P, Moriguchi M, Biochem Biophys Res Commun. 2006 Aug 11;346(4):1118-24. Epub 2006 Jun 6. PMID:[http:// | + | Crystal structure of a major fragment of the salt-tolerant glutaminase from Micrococcus luteus K-3., Yoshimune K, Shirakihara Y, Shiratori A, Wakayama M, Chantawannakul P, Moriguchi M, Biochem Biophys Res Commun. 2006 Aug 11;346(4):1118-24. Epub 2006 Jun 6. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/16793004 16793004] |
[[Category: Glutaminase]] | [[Category: Glutaminase]] | ||
[[Category: Micrococcus luteus]] | [[Category: Micrococcus luteus]] | ||
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[[Category: Yoshimune, K.]] | [[Category: Yoshimune, K.]] | ||
[[Category: alpha/beta structure]] | [[Category: alpha/beta structure]] | ||
| - | [[Category: structural | + | [[Category: structural genomic]] |
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 16:26:47 2008'' |
Revision as of 14:26, 20 March 2008
| |||||||
| , resolution 2.44Å | |||||||
|---|---|---|---|---|---|---|---|
| Activity: | Glutaminase, with EC number 3.5.1.2 | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
Crystal structure of a major fragment of the salt-tolerant glutaminase from Micrococcus luteus K-3
Overview
Glutaminase of Micrococcus luteus K-3 (intact glutaminase; 48kDa) is digested to a C-terminally truncated fragment (glutaminase fragment; 42kDa) that shows higher salt tolerance than that of the intact glutaminase. The crystal structure of the glutaminase fragment was determined at 2.4A resolution using multiple-wavelength anomalous dispersion (MAD). The glutaminase fragment is composed of N-terminal and C-terminal domains, and a putative catalytic serine-lysine dyad (S64 and K67) is located in a cleft of the N-terminal domain. Mutations of the S64 or K67 residues abolished the enzyme activity. The N-terminal domain has abundant glutamic acid residues on its surface, which may explain its salt-tolerant mechanism. A diffraction analysis of the intact glutaminase crystals (a twinning fraction of 0.43) located the glutaminase fragment in the unit cell but failed to turn up clear densities for the missing C-terminal portion of the molecule.
About this Structure
2DFW is a Single protein structure of sequence from Micrococcus luteus. Full crystallographic information is available from OCA.
Reference
Crystal structure of a major fragment of the salt-tolerant glutaminase from Micrococcus luteus K-3., Yoshimune K, Shirakihara Y, Shiratori A, Wakayama M, Chantawannakul P, Moriguchi M, Biochem Biophys Res Commun. 2006 Aug 11;346(4):1118-24. Epub 2006 Jun 6. PMID:16793004
Page seeded by OCA on Thu Mar 20 16:26:47 2008
