1h0d
From Proteopedia
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==Overview== | ==Overview== | ||
| - | The murine monoclonal antibody 26-2F neutralizes the angiogenic and, ribonucleolytic activities of human angiogenin (ANG) and is highly, effective in preventing the establishment and metastatic dissemination of, human tumors in athymic mice. Here we report a 2.0 A resolution crystal, structure for the complex of ANG with the Fab fragment of 26-2F that, reveals the detailed interactions between ANG and the, complementarity-determining regions (CDRs) of the antibody. Surprisingly, Fab binding induces a dramatic conformational change in the cell binding, region of ANG at the opposite end of the molecule from the combining site;, crosslinking experiments indicate that this rearrangement also occurs in, solution. The ANG-Fab complex structure should be invaluable for designing, maximally . | + | The murine monoclonal antibody 26-2F neutralizes the angiogenic and, ribonucleolytic activities of human angiogenin (ANG) and is highly, effective in preventing the establishment and metastatic dissemination of, human tumors in athymic mice. Here we report a 2.0 A resolution crystal, structure for the complex of ANG with the Fab fragment of 26-2F that, reveals the detailed interactions between ANG and the, complementarity-determining regions (CDRs) of the antibody. Surprisingly, Fab binding induces a dramatic conformational change in the cell binding, region of ANG at the opposite end of the molecule from the combining site;, crosslinking experiments indicate that this rearrangement also occurs in, solution. The ANG-Fab complex structure should be invaluable for designing, maximally humanized versions of 26-2F for potential clinical use. |
==About this Structure== | ==About this Structure== | ||
| - | 1H0D is a | + | 1H0D is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] with SO4 and GOL as [http://en.wikipedia.org/wiki/ligands ligands]. Structure known Active Site: SA1. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1H0D OCA]. |
==Reference== | ==Reference== | ||
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[[Category: ribonuclease]] | [[Category: ribonuclease]] | ||
| - | ''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Mon Nov 5 13:34:50 2007'' |
Revision as of 11:29, 5 November 2007
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CRYSTAL STRUCTURE OF HUMAN ANGIOGENIN IN COMPLEX WITH FAB FRAGMENT OF ITS MONOCLONAL ANTIBODY MAB 26-2F
Overview
The murine monoclonal antibody 26-2F neutralizes the angiogenic and, ribonucleolytic activities of human angiogenin (ANG) and is highly, effective in preventing the establishment and metastatic dissemination of, human tumors in athymic mice. Here we report a 2.0 A resolution crystal, structure for the complex of ANG with the Fab fragment of 26-2F that, reveals the detailed interactions between ANG and the, complementarity-determining regions (CDRs) of the antibody. Surprisingly, Fab binding induces a dramatic conformational change in the cell binding, region of ANG at the opposite end of the molecule from the combining site;, crosslinking experiments indicate that this rearrangement also occurs in, solution. The ANG-Fab complex structure should be invaluable for designing, maximally humanized versions of 26-2F for potential clinical use.
About this Structure
1H0D is a Single protein structure of sequence from Homo sapiens with SO4 and GOL as ligands. Structure known Active Site: SA1. Full crystallographic information is available from OCA.
Reference
The crystal structure of human angiogenin in complex with an antitumor neutralizing antibody., Chavali GB, Papageorgiou AC, Olson KA, Fett JW, Hu G, Shapiro R, Acharya KR, Structure. 2003 Jul;11(7):875-85. PMID:12842050
Page seeded by OCA on Mon Nov 5 13:34:50 2007
