1pt3

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[[Image:1pt3.png|left|200px]]
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==Crystal structures of nuclease-ColE7 complexed with octamer DNA==
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<StructureSection load='1pt3' size='340' side='right' caption='[[1pt3]], [[Resolution|resolution]] 2.50&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[1pt3]] is a 8 chain structure with sequence from [http://en.wikipedia.org/wiki/Escherichia_coli_str._k-12_substr._w3110 Escherichia coli str. k-12 substr. w3110]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1PT3 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1PT3 FirstGlance]. <br>
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</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[7cei|7cei]], [[1m08|1m08]], [[1mz8|1mz8]]</td></tr>
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<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">COLE7 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=316407 Escherichia coli str. K-12 substr. W3110])</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1pt3 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1pt3 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1pt3 RCSB], [http://www.ebi.ac.uk/pdbsum/1pt3 PDBsum]</span></td></tr>
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</table>
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== Function ==
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[[http://www.uniprot.org/uniprot/CEA7_ECOLX CEA7_ECOLX]] This plasmid-coded bactericidal protein is an endonuclease active on both single- and double-stranded DNA but with undefined specificity. Colicins are polypeptide toxins produced by and active against E.coli and closely related bacteria.
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/pt/1pt3_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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The bacterial toxin ColE7 bears an HNH motif which has been identified in hundreds of prokaryotic and eukaryotic endonucleases, involved in DNA homing, restriction, repair, or chromosome degradation. The crystal structure of the nuclease domain of ColE7 in complex with a duplex DNA has been determined at 2.5 A resolution. The HNH motif is bound at the minor groove primarily to DNA phosphate groups at and beyond the 3' side of the scissile phosphate, with little interaction with ribose groups and bases. This result provides a structural basis for sugar- and sequence-independent DNA recognition and the inhibition mechanism by inhibitor Im7, which blocks the substrate binding site but not the active site. Structural comparison shows that two families of endonucleases bind and bend DNA in a similar way to that of the HNH ColE7, indicating that endonucleases containing a "betabetaalpha-metal" fold of active site possess a universal mode for protein-DNA interactions.
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{{STRUCTURE_1pt3| PDB=1pt3 | SCENE= }}
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DNA binding and degradation by the HNH protein ColE7.,Hsia KC, Chak KF, Liang PH, Cheng YS, Ku WY, Yuan HS Structure. 2004 Feb;12(2):205-14. PMID:14962381<ref>PMID:14962381</ref>
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===Crystal structures of nuclease-ColE7 complexed with octamer DNA===
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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{{ABSTRACT_PUBMED_14962381}}
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==About this Structure==
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[[1pt3]] is a 8 chain structure with sequence from [http://en.wikipedia.org/wiki/Escherichia_coli_str._k-12_substr._w3110 Escherichia coli str. k-12 substr. w3110]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1PT3 OCA].
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==See Also==
==See Also==
*[[Colicin|Colicin]]
*[[Colicin|Colicin]]
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== References ==
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==Reference==
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<references/>
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<ref group="xtra">PMID:014962381</ref><references group="xtra"/>
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__TOC__
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</StructureSection>
[[Category: Escherichia coli str. k-12 substr. w3110]]
[[Category: Escherichia coli str. k-12 substr. w3110]]
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[[Category: Chak, K F.]]
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[[Category: Chak, K F]]
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[[Category: Cheng, Y S.]]
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[[Category: Cheng, Y S]]
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[[Category: Hsia, K C.]]
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[[Category: Hsia, K C]]
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[[Category: Ku, W Y.]]
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[[Category: Ku, W Y]]
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[[Category: Yuan, H S.]]
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[[Category: Yuan, H S]]
[[Category: Colicin]]
[[Category: Colicin]]
[[Category: Endonuclease]]
[[Category: Endonuclease]]

Revision as of 13:37, 25 December 2014

Crystal structures of nuclease-ColE7 complexed with octamer DNA

1pt3, resolution 2.50Å

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