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3kyh

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Revision as of 13:42, 25 December 2014

Saccharomyces cerevisiae Cet1-Ceg1 capping apparatus

Structural highlights

3kyh is a 4 chain structure with sequence from Saccharomyces cerevisiae. The January 2012 RCSB PDB Molecule of the Month feature on Messenger RNA Capping by David Goodsell is 10.2210/rcsb_pdb/mom_2012_1. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Gene:	CET1, P1433, YPL228W (Saccharomyces cerevisiae), CEG1, G2853, YGL130W (Saccharomyces cerevisiae)
Activity:	Polynucleotide 5'-phosphatase, with EC number 3.1.3.33
Resources:	FirstGlance, OCA, RCSB, PDBsum

Function

[CET1_YEAST] First step of mRNA capping. Converts the 5'-triphosphate end of a nascent mRNA chain into a diphosphate end.^[1] [MCE1_YEAST] Second step of mRNA capping. Transfer of the GMP moiety of GTP to the 5'-end of RNA via an enzyme-GMP covalent reaction intermediate.

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

Publication Abstract from PubMed

The 5' guanine-N7 cap is the first cotranscriptional modification of messenger RNA. In Saccharomyces cerevisiae, the first two steps in capping are catalyzed by the RNA triphosphatase Cet1 and RNA guanylyltransferase Ceg1, which form a complex that is directly recruited to phosphorylated RNA polymerase II (RNAP IIo), primarily via contacts between RNAP IIo and Ceg1. A 3.0 A crystal structure of Cet1-Ceg1 revealed a 176 kDa heterotetrameric complex composed of one Cet1 homodimer that associates with two Ceg1 molecules via interactions between the Ceg1 oligonucleotide binding domain and an extended Cet1 WAQKW amino acid motif. The WAQKW motif is followed by a flexible linker that would allow Ceg1 to achieve conformational changes required for capping while maintaining interactions with both Cet1 and RNAP IIo. The impact of mutations as assessed through genetic analysis in S. cerevisiae is consonant with contacts observed in the Cet1-Ceg1 structure.

Structure of the Saccharomyces cerevisiae Cet1-Ceg1 mRNA capping apparatus.,Gu M, Rajashankar KR, Lima CD Structure. 2010 Feb 10;18(2):216-27. PMID:20159466^[2]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Hausmann S, Pei Y, Shuman S. Homodimeric quaternary structure is required for the in vivo function and thermal stability of Saccharomyces cerevisiae and Schizosaccharomyces pombe RNA triphosphatases. J Biol Chem. 2003 Aug 15;278(33):30487-96. Epub 2003 Jun 3. PMID:12788946 doi:http://dx.doi.org/10.1074/jbc.M303060200
↑ Gu M, Rajashankar KR, Lima CD. Structure of the Saccharomyces cerevisiae Cet1-Ceg1 mRNA capping apparatus. Structure. 2010 Feb 10;18(2):216-27. PMID:20159466 doi:10.1016/j.str.2009.12.009

1 Structural highlights
2 Function
3 Evolutionary Conservation
4 Publication Abstract from PubMed
5 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/3kyh"

@@ Line 7: / Line 7: @@
 <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3kyh FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3kyh OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3kyh RCSB], [http://www.ebi.ac.uk/pdbsum/3kyh PDBsum]</span></td></tr>
 </table>
+== Function ==
+[[http://www.uniprot.org/uniprot/CET1_YEAST CET1_YEAST]] First step of mRNA capping. Converts the 5'-triphosphate end of a nascent mRNA chain into a diphosphate end.<ref>PMID:12788946</ref>  [[http://www.uniprot.org/uniprot/MCE1_YEAST MCE1_YEAST]] Second step of mRNA capping. Transfer of the GMP moiety of GTP to the 5'-end of RNA via an enzyme-GMP covalent reaction intermediate.
 == Evolutionary Conservation ==
 [[Image:Consurf_key_small.gif|200px|right]]

3kyh

From Proteopedia

Revision as of 13:42, 25 December 2014

Saccharomyces cerevisiae Cet1-Ceg1 capping apparatus

Structural highlights

Function

Evolutionary Conservation

Publication Abstract from PubMed

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

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