2dob
From Proteopedia
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| - | [[Image:2dob.gif|left|200px]] | + | [[Image:2dob.gif|left|200px]] |
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| - | '''Crystal Structure of Human Saposin A''' | + | {{Structure |
| + | |PDB= 2dob |SIZE=350|CAPTION= <scene name='initialview01'>2dob</scene>, resolution 2.00Å | ||
| + | |SITE= | ||
| + | |LIGAND= <scene name='pdbligand=CA:CALCIUM ION'>CA</scene> | ||
| + | |ACTIVITY= | ||
| + | |GENE= PSAP ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 Homo sapiens]) | ||
| + | }} | ||
| + | |||
| + | '''Crystal Structure of Human Saposin A''' | ||
| + | |||
==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
| - | 2DOB is a [ | + | 2DOB is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2DOB OCA]. |
==Reference== | ==Reference== | ||
| - | Crystal structures of saposins A and C., Ahn VE, Leyko P, Alattia JR, Chen L, Prive GG, Protein Sci. 2006 Aug;15(8):1849-57. Epub 2006 Jul 5. PMID:[http:// | + | Crystal structures of saposins A and C., Ahn VE, Leyko P, Alattia JR, Chen L, Prive GG, Protein Sci. 2006 Aug;15(8):1849-57. Epub 2006 Jul 5. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/16823039 16823039] |
[[Category: Homo sapiens]] | [[Category: Homo sapiens]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
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[[Category: sphingolipid activator protein]] | [[Category: sphingolipid activator protein]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 16:29:37 2008'' |
Revision as of 14:29, 20 March 2008
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| , resolution 2.00Å | |||||||
|---|---|---|---|---|---|---|---|
| Ligands: | |||||||
| Gene: | PSAP (Homo sapiens) | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
Crystal Structure of Human Saposin A
Contents |
Overview
Saposins A and C are sphingolipid activator proteins required for the lysosomal breakdown of galactosylceramide and glucosylceramide, respectively. The saposins interact with lipids, leading to an enhanced accessibility of the lipid headgroups to their cognate hydrolases. We have determined the crystal structures of human saposins A and C to 2.0 Angstroms and 2.4 Angstroms, respectively, and both reveal the compact, monomeric saposin fold. We confirmed that these two proteins were monomeric in solution at pH 7.0 by analytical centrifugation. However, at pH 4.8, in the presence of the detergent C(8)E(5), saposin A assembled into dimers, while saposin C formed trimers. Saposin B was dimeric under all conditions tested. The self-association of the saposins is likely to be relevant to how these small proteins interact with lipids, membranes, and hydrolase enzymes.
Disease
Known diseases associated with this structure: Combined SAP deficiency OMIM:[176801], Gaucher disease, atypical OMIM:[176801], Krabbe disease, atypical OMIM:[176801], Metachromatic leukodystrophy due to SAP-b deficiency OMIM:[176801]
About this Structure
2DOB is a Single protein structure of sequence from Homo sapiens. Full crystallographic information is available from OCA.
Reference
Crystal structures of saposins A and C., Ahn VE, Leyko P, Alattia JR, Chen L, Prive GG, Protein Sci. 2006 Aug;15(8):1849-57. Epub 2006 Jul 5. PMID:16823039
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