3pwt

(Difference between revisions)

Revision as of 14:13, 25 December 2014

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 <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3pwt FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3pwt OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3pwt RCSB], [http://www.ebi.ac.uk/pdbsum/3pwt PDBsum]</span></td></tr>
 </table>
+== Function ==
+[[http://www.uniprot.org/uniprot/TOP1_ECOLI TOP1_ECOLI]] Releases the supercoiling and torsional tension of DNA, which is introduced during the DNA replication and transcription, by transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand break via transesterification at a target site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(5'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 3'-OH DNA strand. The free DNA strand then undergoes passage around the unbroken strand, thus removing DNA supercoils. Finally, in the religation step, the DNA 3'-OH attacks the covalent intermediate to expel the active-site tyrosine and restore the DNA phosphodiester backbone.<ref>PMID:9497321</ref> <ref>PMID:10681504</ref> <ref>PMID:21482796</ref>
 ==See Also==
 *[[Topoisomerase|Topoisomerase]]
+== References ==
+<references/>
 __TOC__
 </StructureSection>