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2du0

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[[Image:2du0.gif|left|200px]]<br /><applet load="2du0" size="350" color="white" frame="true" align="right" spinBox="true"
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[[Image:2du0.gif|left|200px]]
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caption="2du0, resolution 2.70&Aring;" />
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'''Crystal structure of basic winged bean lectin in complex with Alpha-D-galactose'''<br />
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{{Structure
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|PDB= 2du0 |SIZE=350|CAPTION= <scene name='initialview01'>2du0</scene>, resolution 2.70&Aring;
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|SITE=
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|LIGAND= <scene name='pdbligand=GLA:ALPHA+D-GALACTOSE'>GLA</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene>, <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene> and <scene name='pdbligand=MN:MANGANESE (II) ION'>MN</scene>
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|ACTIVITY=
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|GENE=
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}}
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'''Crystal structure of basic winged bean lectin in complex with Alpha-D-galactose'''
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==Overview==
==Overview==
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==About this Structure==
==About this Structure==
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2DU0 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Psophocarpus_tetragonolobus Psophocarpus tetragonolobus] with <scene name='pdbligand=GLA:'>GLA</scene>, <scene name='pdbligand=NAG:'>NAG</scene>, <scene name='pdbligand=CA:'>CA</scene> and <scene name='pdbligand=MN:'>MN</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2DU0 OCA].
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2DU0 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Psophocarpus_tetragonolobus Psophocarpus tetragonolobus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2DU0 OCA].
==Reference==
==Reference==
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Structural basis for the carbohydrate-specificity of basic winged-bean lectin and its differential affinity for Gal and GalNAc., Kulkarni KA, Katiyar S, Surolia A, Vijayan M, Suguna K, Acta Crystallogr D Biol Crystallogr. 2006 Nov;62(Pt 11):1319-24. Epub 2006, Oct 18. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=17057334 17057334]
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Structural basis for the carbohydrate-specificity of basic winged-bean lectin and its differential affinity for Gal and GalNAc., Kulkarni KA, Katiyar S, Surolia A, Vijayan M, Suguna K, Acta Crystallogr D Biol Crystallogr. 2006 Nov;62(Pt 11):1319-24. Epub 2006, Oct 18. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/17057334 17057334]
[[Category: Psophocarpus tetragonolobus]]
[[Category: Psophocarpus tetragonolobus]]
[[Category: Single protein]]
[[Category: Single protein]]
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[[Category: legume lectin]]
[[Category: legume lectin]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 17:02:36 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 16:31:29 2008''

Revision as of 14:31, 20 March 2008

Image:2du0.gif


PDB ID 2du0

Drag the structure with the mouse to rotate
, resolution 2.70Å
Ligands: , , and
Coordinates: save as pdb, mmCIF, xml



Crystal structure of basic winged bean lectin in complex with Alpha-D-galactose


Overview

The crystal structure of the complexes of basic winged-bean lectin with galactose, 2-methoxygalactose, N-acetylgalactosamine and methyl-alpha-N-acetylgalactosamine have been determined. Lectin-sugar interactions involve four hydrogen bonds and a stacking interaction in all of the complexes. In addition, an N-H...O hydrogen bond involving the hydroxyl group at C2 exists in the galactose and 2-methoxygalactose complexes. An additional hydrophobic interaction involving the methyl group in the latter leads to the higher affinity of the methyl derivative. In the lectin-N-acetylgalactosamine complex the N-H...O hydrogen bond is lost, but a compensatory hydrogen bond is formed involving the O atom of the acetamido group. In addition, the CH(3) moiety of the acetamido group is involved in hydrophobic interactions. Consequently, the 2-methyl and acetamido derivatives of galactose have nearly the same affinity for the lectin. The methyl group alpha-linked to the galactose takes part in additional hydrophobic interactions. Therefore, methyl-alpha-N-acetylgalactosamine has a higher affinity than N-acetylgalactosamine for the lectin. The structures of basic winged-bean lectin-sugar complexes provide a framework for examining the relative affinity of galactose and galactosamine for the lectins that bind to them. The complexes also lead to a structural explanation for the blood-group specificity of basic winged-bean lectin.

About this Structure

2DU0 is a Single protein structure of sequence from Psophocarpus tetragonolobus. Full crystallographic information is available from OCA.

Reference

Structural basis for the carbohydrate-specificity of basic winged-bean lectin and its differential affinity for Gal and GalNAc., Kulkarni KA, Katiyar S, Surolia A, Vijayan M, Suguna K, Acta Crystallogr D Biol Crystallogr. 2006 Nov;62(Pt 11):1319-24. Epub 2006, Oct 18. PMID:17057334

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