4ebs

From Proteopedia

(Difference between revisions)

Revision as of 14:42, 25 December 2014

Structural studies on the molecular interactions between camel Peptidoglycan Recognition Protein, CPGRP-S and peptidoglycan moieties, N-Acetylglucosamine and N-Acetylmuramic acid

Structural highlights

4ebs is a 4 chain structure with sequence from Camelus dromedarius. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Ligands:	, ,
Related:	4ebt
Resources:	FirstGlance, OCA, RCSB, PDBsum

Function

[PGRP1_CAMDR] Pattern receptor that binds to murein peptidoglycans (PGN) of Gram-positive bacteria. Has bactericidal activity towards Gram-positive bacteria. May kill Gram-positive bacteria by interfering with peptidoglycan biosynthesis. Binds also to Gram-negative bacteria. Involved in innate immunity. Is microbicidal for Gram-positive and Gram-negative bacteria and yeast. May function in intracellular killing of bacteria (By similarity).

Publication Abstract from PubMed

Peptidoglycan (PGN) consists of repeating units of N-acetylglucosamine (GlcNAc) and N-acetylmuramic acid (MurNAc), which are cross-linked by short peptides. It is well known that PGN forms a major cell wall component of bacteria making it an important ligand for the recognition by peptidoglycan recognition proteins (PGRPs) of the host. The binding studies showed that PGN, GlcNAc, and MurNAc bind to camel PGRP-S (CPGRP-S) with affinities corresponding to dissociation constants of 1.3 x 10(-9), 2.6 x 10(-7), and 1.8 x 10(-7) M, respectively. The crystal structure determinations of the complexes of CPGRP-S with GlcNAc and MurNAc showed that the structures consist of four crystallographically independent molecules, A, B, C, and D, in the asymmetric unit that exists as A-B and C-D units of two neighboring linear polymers. The structure determinations showed that compounds GlcNAc and MurNAc bound to CPGRP-S at the same subsite in molecule C. Both GlcNAc and MurNAc form several hydrogen bonds and extensive hydrophobic interactions with protein atoms, indicating the specific nature of their bindings. Flow cytometric studies showed that PGN enhanced the secretions of TNF-alpha and IL-6 from human peripheral blood mononuclear cells. The introduction of CPGRP-S to the PGN-challenged cultured peripheral blood mononuclear cells reduced the expressions of proinflammatory cytokines, TNF-alpha and IL-6. This showed that CPGRP-S inhibited PGN-induced production of proinflammatory cytokines and down-regulated macrophage-mediated inflammation, indicating its potential applications as an antibacterial agent.

Structural studies on molecular interactions between camel peptidoglycan recognition protein, CPGRP-S, and peptidoglycan moieties N-acetylglucosamine and N-acetylmuramic acid.,Sharma P, Yamini S, Dube D, Singh A, Sinha M, Dey S, Mitra DK, Kaur P, Sharma S, Singh TP J Biol Chem. 2012 Jun 22;287(26):22153-64. Epub 2012 May 9. PMID:22573327^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Sharma P, Yamini S, Dube D, Singh A, Sinha M, Dey S, Mitra DK, Kaur P, Sharma S, Singh TP. Structural studies on molecular interactions between camel peptidoglycan recognition protein, CPGRP-S, and peptidoglycan moieties N-acetylglucosamine and N-acetylmuramic acid. J Biol Chem. 2012 Jun 22;287(26):22153-64. Epub 2012 May 9. PMID:22573327 doi:10.1074/jbc.M111.321307

1 Structural highlights
2 Function
3 Publication Abstract from PubMed
4 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/4ebs"

@@ Line 3: / Line 3: @@
 == Structural highlights ==
 <table><tr><td colspan='2'>[[4ebs]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Camelus_dromedarius Camelus dromedarius]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4EBS OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4EBS FirstGlance]. <br>
-</td></tr><tr><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=AMU:BETA-N-ACETYLMURAMIC+ACID'>AMU</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=TLA:L(+)-TARTARIC+ACID'>TLA</scene><br>
+</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=AMU:BETA-N-ACETYLMURAMIC+ACID'>AMU</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=TLA:L(+)-TARTARIC+ACID'>TLA</scene></td></tr>
-<tr><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[4ebt|4ebt]]</td></tr>
+<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[4ebt|4ebt]]</td></tr>
-<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4ebs FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4ebs OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4ebs RCSB], [http://www.ebi.ac.uk/pdbsum/4ebs PDBsum]</span></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4ebs FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4ebs OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4ebs RCSB], [http://www.ebi.ac.uk/pdbsum/4ebs PDBsum]</span></td></tr>
-<table>
+</table>
+== Function ==
+[[http://www.uniprot.org/uniprot/PGRP1_CAMDR PGRP1_CAMDR]] Pattern receptor that binds to murein peptidoglycans (PGN) of Gram-positive bacteria. Has bactericidal activity towards Gram-positive bacteria. May kill Gram-positive bacteria by interfering with peptidoglycan biosynthesis. Binds also to Gram-negative bacteria. Involved in innate immunity. Is microbicidal for Gram-positive and Gram-negative bacteria and yeast. May function in intracellular killing of bacteria (By similarity).
 <div style="background-color:#fffaf0;">
 == Publication Abstract from PubMed ==
@@ Line 20: / Line 22: @@
 </StructureSection>
 [[Category: Camelus dromedarius]]
-[[Category: Dube, D.]]
+[[Category: Dube, D]]
-[[Category: Kaur, P.]]
+[[Category: Kaur, P]]
-[[Category: Sharma, P.]]
+[[Category: Sharma, P]]
-[[Category: Sharma, S.]]
+[[Category: Sharma, S]]
-[[Category: Singh, T P.]]
+[[Category: Singh, T P]]
-[[Category: Sinha, M.]]
+[[Category: Sinha, M]]
-[[Category: Yamini, S.]]
+[[Category: Yamini, S]]
 [[Category: Antibiotic]]
 [[Category: Antimicrobial]]

4ebs

From Proteopedia

Revision as of 14:42, 25 December 2014

Structural studies on the molecular interactions between camel Peptidoglycan Recognition Protein, CPGRP-S and peptidoglycan moieties, N-Acetylglucosamine and N-Acetylmuramic acid

Structural highlights

Function

Publication Abstract from PubMed

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

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