2m8f
From Proteopedia
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| - | + | ==Structure of lasso peptide astexin3== | |
| - | === | + | <StructureSection load='2m8f' size='340' side='right' caption='[[2m8f]], [[NMR_Ensembles_of_Models | 20 NMR models]]' scene=''> |
| - | + | == Structural highlights == | |
| + | <table><tr><td colspan='2'>[[2m8f]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Asticcacaulis_excentricus_cb_48 Asticcacaulis excentricus cb 48]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2M8F OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2M8F FirstGlance]. <br> | ||
| + | </td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">Astex_2447 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=573065 Asticcacaulis excentricus CB 48])</td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2m8f FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2m8f OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2m8f RCSB], [http://www.ebi.ac.uk/pdbsum/2m8f PDBsum]</span></td></tr> | ||
| + | </table> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | Lasso peptides are a class of ribosomally-derived natural products with diverse bioactivities. The characteristic threaded lasso structure in these peptides derives from an isopeptide bond attaching the N-terminus of the peptide to an acidic sidechain. Here we describe the heterologous expression of a lasso peptide gene cluster encoding two lasso peptides, astexin-2 and astexin-3 and solve the solution structure of astexin-3. This cluster also encodes an enzyme annotated as a protease. We show that this enzyme, AtxE2, is a lasso peptide isopeptidase that specifically hydrolyzes astexins-2 and -3 converting them to linear peptides. Astexin-3 is highly thermostable and resists unthreading after extensive heat treatment. In contrast, astexin-2 unthreads upon heat treatment. AtxE2 has no activity toward unthreaded astexin-2 demonstrating that this isopeptidase must recognize a knotted structure in order to function. We also use this isopeptidase as a tool to study evolutionary relationships between lasso peptide gene clusters. | ||
| - | + | Discovery and Characterization of an Isopeptidase that Linearizes Lasso Peptides.,Maksimov MO, Link AJ J Am Chem Soc. 2013 Jul 17. PMID:23862624<ref>PMID:23862624</ref> | |
| - | + | ||
| - | == | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> |
| - | + | </div> | |
| + | == References == | ||
| + | <references/> | ||
| + | __TOC__ | ||
| + | </StructureSection> | ||
[[Category: Asticcacaulis excentricus cb 48]] | [[Category: Asticcacaulis excentricus cb 48]] | ||
| - | [[Category: Link, A | + | [[Category: Link, A]] |
| - | [[Category: Maksimov, M O | + | [[Category: Maksimov, M O]] |
[[Category: Sidechain-to-backbone link]] | [[Category: Sidechain-to-backbone link]] | ||
[[Category: Unknown function]] | [[Category: Unknown function]] | ||
Revision as of 14:49, 25 December 2014
Structure of lasso peptide astexin3
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