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2f52

From Proteopedia

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[[Image:2f52.png|left|200px]]
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==Solution structure of cold shock protein CspB from Bacillus subtilis in complex with heptathymidine==
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<StructureSection load='2f52' size='340' side='right' caption='[[2f52]], [[NMR_Ensembles_of_Models | 18 NMR models]]' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[2f52]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Bacillus_subtilis Bacillus subtilis]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2F52 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2F52 FirstGlance]. <br>
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</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[1csp|1csp]], [[1nmf|1nmf]]</td></tr>
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<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">cspB, cspA ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1423 Bacillus subtilis])</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2f52 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2f52 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2f52 RCSB], [http://www.ebi.ac.uk/pdbsum/2f52 PDBsum]</span></td></tr>
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</table>
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== Function ==
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[[http://www.uniprot.org/uniprot/CSPB_BACSU CSPB_BACSU]] Binds to the pentamer sequences ATTGG and CCAAT with highest affinity in single-stranded DNA, and also to other sequences. Has greater affinity for ATTGG than CCAAT. Can act as transcriptional activator of cold shock genes by recognizing putative ATTGG-box elements present in promoter regions of genes induced under cold shock conditions.
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/f5/2f52_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Cold shock proteins (CSP) belong to the family of single-stranded nucleic acid binding proteins with OB-fold. CSP are believed to function as 'RNA chaperones' and during anti-termination. We determined the solution structure of Bs-CspB bound to the single-stranded DNA (ssDNA) fragment heptathymidine (dT7) by NMR spectroscopy. Bs-CspB reveals an almost invariant conformation when bound to dT7 with only minor reorientations in loop beta1-beta2 and beta3-beta4 and of few aromatic side chains involved in base stacking. Binding studies of protein variants and mutated ssDNA demonstrated that Bs-CspB associates with ssDNA at almost diffusion controlled rates and low sequence specificity consistent with its biological function. A variation of the ssDNA affinity is accomplished solely by changes of the dissociation rate. 15N NMR relaxation and H/D exchange experiments revealed that binding of dT7 increases the stability of Bs-CspB and reduces the sub-nanosecond dynamics of the entire protein and especially of loop beta3-beta4.
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{{STRUCTURE_2f52| PDB=2f52 | SCENE= }}
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Recognition of T-rich single-stranded DNA by the cold shock protein Bs-CspB in solution.,Zeeb M, Max KE, Weininger U, Low C, Sticht H, Balbach J Nucleic Acids Res. 2006;34(16):4561-71. Epub 2006 Sep 6. PMID:16956971<ref>PMID:16956971</ref>
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===Solution structure of cold shock protein CspB from Bacillus subtilis in complex with heptathymidine===
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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{{ABSTRACT_PUBMED_16956971}}
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== References ==
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<references/>
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==About this Structure==
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__TOC__
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[[2f52]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Bacillus_subtilis Bacillus subtilis]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2F52 OCA].
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</StructureSection>
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==Reference==
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<ref group="xtra">PMID:016956971</ref><references group="xtra"/>
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[[Category: Bacillus subtilis]]
[[Category: Bacillus subtilis]]
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[[Category: Balbach, J.]]
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[[Category: Balbach, J]]
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[[Category: Sticht, H.]]
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[[Category: Sticht, H]]
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[[Category: Zeeb, M.]]
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[[Category: Zeeb, M]]
[[Category: Beta barrel]]
[[Category: Beta barrel]]
[[Category: Dna binding protein-transcription complex]]
[[Category: Dna binding protein-transcription complex]]
[[Category: Ob-fold]]
[[Category: Ob-fold]]

Revision as of 14:53, 25 December 2014

Solution structure of cold shock protein CspB from Bacillus subtilis in complex with heptathymidine

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