4jal

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{{STRUCTURE_4jal| PDB=4jal | SCENE= }}
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==Crystal structure of tRNA (Um34/Cm34) methyltransferase TrmL from Escherichia coli with SAH==
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===Crystal structure of tRNA (Um34/Cm34) methyltransferase TrmL from Escherichia coli with SAH===
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<StructureSection load='4jal' size='340' side='right' caption='[[4jal]], [[Resolution|resolution]] 2.00&Aring;' scene=''>
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{{ABSTRACT_PUBMED_23804755}}
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== Structural highlights ==
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<table><tr><td colspan='2'>[[4jal]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Ecoli Ecoli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4JAL OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4JAL FirstGlance]. <br>
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==Function==
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</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=EPE:4-(2-HYDROXYETHYL)-1-PIPERAZINE+ETHANESULFONIC+ACID'>EPE</scene>, <scene name='pdbligand=SAH:S-ADENOSYL-L-HOMOCYSTEINE'>SAH</scene></td></tr>
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<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[4jak|4jak]]</td></tr>
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<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">trmL, yibK, b3606, JW3581 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=83333 ECOLI])</td></tr>
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<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/tRNA_(cytidine(34)-2'-O)-methyltransferase tRNA (cytidine(34)-2'-O)-methyltransferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.1.1.207 2.1.1.207] </span></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4jal FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4jal OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4jal RCSB], [http://www.ebi.ac.uk/pdbsum/4jal PDBsum]</span></td></tr>
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</table>
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== Function ==
[[http://www.uniprot.org/uniprot/TRML_ECOLI TRML_ECOLI]] Methylates the ribose at the nucleotide 34 wobble position in the two leucyl isoacceptors tRNA(Leu)(CmAA) and tRNA(Leu)(cmnm5UmAA). Catalyzes the methyl transfer from S-adenosyl-L-methionine to the 2'-OH of the wobble nucleotide. Recognition of the target requires a pyridine at position 34 and N(6)-(isopentenyl)-2-methylthioadenosine at position 37.<ref>PMID:20855540</ref>
[[http://www.uniprot.org/uniprot/TRML_ECOLI TRML_ECOLI]] Methylates the ribose at the nucleotide 34 wobble position in the two leucyl isoacceptors tRNA(Leu)(CmAA) and tRNA(Leu)(cmnm5UmAA). Catalyzes the methyl transfer from S-adenosyl-L-methionine to the 2'-OH of the wobble nucleotide. Recognition of the target requires a pyridine at position 34 and N(6)-(isopentenyl)-2-methylthioadenosine at position 37.<ref>PMID:20855540</ref>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Unlike other transfer RNAs (tRNA)-modifying enzymes from the SPOUT methyltransferase superfamily, the tRNA (Um34/Cm34) methyltransferase TrmL lacks the usual extension domain for tRNA binding and consists only of a SPOUT domain. Both the catalytic and tRNA recognition mechanisms of this enzyme remain elusive. By using tRNAs purified from an Escherichia coli strain with the TrmL gene deleted, we found that TrmL can independently catalyze the methyl transfer from S-adenosyl-L-methionine to and isoacceptors without the involvement of other tRNA-binding proteins. We have solved the crystal structures of TrmL in apo form and in complex with S-adenosyl-homocysteine and identified the cofactor binding site and a possible active site. Methyltransferase activity and tRNA-binding affinity of TrmL mutants were measured to identify residues important for tRNA binding of TrmL. Our results suggest that TrmL functions as a homodimer by using the conserved C-terminal half of the SPOUT domain for catalysis, whereas residues from the less-conserved N-terminal half of the other subunit participate in tRNA recognition.
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The tRNA recognition mechanism of the minimalist SPOUT methyltransferase, TrmL.,Liu RJ, Zhou M, Fang ZP, Wang M, Zhou XL, Wang ED Nucleic Acids Res. 2013 Jun 26. PMID:23804755<ref>PMID:23804755</ref>
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==About this Structure==
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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[[4jal]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Ecoli Ecoli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4JAL OCA].
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</div>
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==Reference==
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==See Also==
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<ref group="xtra">PMID:023804755</ref><references group="xtra"/><references/>
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*[[TRNA methyltransferase|TRNA methyltransferase]]
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== References ==
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<references/>
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__TOC__
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</StructureSection>
[[Category: Ecoli]]
[[Category: Ecoli]]
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[[Category: Liu, R J.]]
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[[Category: Liu, R J]]
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[[Category: Wang, E D.]]
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[[Category: Wang, E D]]
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[[Category: Zhou, M.]]
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[[Category: Zhou, M]]
[[Category: Deep trefoil knot]]
[[Category: Deep trefoil knot]]
[[Category: Methylation]]
[[Category: Methylation]]

Revision as of 14:56, 25 December 2014

Crystal structure of tRNA (Um34/Cm34) methyltransferase TrmL from Escherichia coli with SAH

4jal, resolution 2.00Å

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