2e5l
From Proteopedia
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| - | [[Image:2e5l.gif|left|200px]] | + | [[Image:2e5l.gif|left|200px]] |
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| - | '''A snapshot of the 30S ribosomal subunit capturing mRNA via the Shine- Dalgarno interaction''' | + | {{Structure |
| + | |PDB= 2e5l |SIZE=350|CAPTION= <scene name='initialview01'>2e5l</scene>, resolution 3.300Å | ||
| + | |SITE= | ||
| + | |LIGAND= <scene name='pdbligand=ZN:ZINC ION'>ZN</scene> | ||
| + | |ACTIVITY= | ||
| + | |GENE= | ||
| + | }} | ||
| + | |||
| + | '''A snapshot of the 30S ribosomal subunit capturing mRNA via the Shine- Dalgarno interaction''' | ||
| + | |||
==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
| - | 2E5L is a [ | + | 2E5L is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/Thermus_thermophilus Thermus thermophilus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2E5L OCA]. |
==Reference== | ==Reference== | ||
| - | A snapshot of the 30S ribosomal subunit capturing mRNA via the Shine-Dalgarno interaction., Kaminishi T, Wilson DN, Takemoto C, Harms JM, Kawazoe M, Schluenzen F, Hanawa-Suetsugu K, Shirouzu M, Fucini P, Yokoyama S, Structure. 2007 Mar;15(3):289-97. PMID:[http:// | + | A snapshot of the 30S ribosomal subunit capturing mRNA via the Shine-Dalgarno interaction., Kaminishi T, Wilson DN, Takemoto C, Harms JM, Kawazoe M, Schluenzen F, Hanawa-Suetsugu K, Shirouzu M, Fucini P, Yokoyama S, Structure. 2007 Mar;15(3):289-97. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/17355865 17355865] |
[[Category: Protein complex]] | [[Category: Protein complex]] | ||
[[Category: Thermus thermophilus]] | [[Category: Thermus thermophilus]] | ||
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[[Category: 5' untranslated region]] | [[Category: 5' untranslated region]] | ||
[[Category: mrna capture]] | [[Category: mrna capture]] | ||
| - | [[Category: national project on protein structural and functional | + | [[Category: national project on protein structural and functional analyse]] |
[[Category: nppsfa]] | [[Category: nppsfa]] | ||
[[Category: riken structural genomics/proteomics initiative]] | [[Category: riken structural genomics/proteomics initiative]] | ||
[[Category: rsgi]] | [[Category: rsgi]] | ||
[[Category: shine-dalgarno interaction]] | [[Category: shine-dalgarno interaction]] | ||
| - | [[Category: structural | + | [[Category: structural genomic]] |
[[Category: translation initiation]] | [[Category: translation initiation]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 16:35:38 2008'' |
Revision as of 14:35, 20 March 2008
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| , resolution 3.300Å | |||||||
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| Ligands: | |||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
A snapshot of the 30S ribosomal subunit capturing mRNA via the Shine- Dalgarno interaction
Overview
In the initiation phase of bacterial translation, the 30S ribosomal subunit captures mRNA in preparation for binding with initiator tRNA. The purine-rich Shine-Dalgarno (SD) sequence, in the 5' untranslated region of the mRNA, anchors the 30S subunit near the start codon, via base pairing with an anti-SD (aSD) sequence at the 3' terminus of 16S rRNA. Here, we present the 3.3 A crystal structure of the Thermus thermophilus 30S subunit bound with an mRNA mimic. The duplex formed by the SD and aSD sequences is snugly docked in a "chamber" between the head and platform domains, demonstrating how the 30S subunit captures and stabilizes the otherwise labile SD helix. This location of the SD helix is suitable for the placement of the start codon AUG in the immediate vicinity of the mRNA channel, in agreement with reported crosslinks between the second position of the start codon and G1530 of 16S rRNA.
About this Structure
2E5L is a Protein complex structure of sequences from Thermus thermophilus. Full crystallographic information is available from OCA.
Reference
A snapshot of the 30S ribosomal subunit capturing mRNA via the Shine-Dalgarno interaction., Kaminishi T, Wilson DN, Takemoto C, Harms JM, Kawazoe M, Schluenzen F, Hanawa-Suetsugu K, Shirouzu M, Fucini P, Yokoyama S, Structure. 2007 Mar;15(3):289-97. PMID:17355865
Page seeded by OCA on Thu Mar 20 16:35:38 2008
Categories: Protein complex | Thermus thermophilus | Fucini, P. | Hanawa-Suetsugu, K. | Harms, J M. | Kaminishi, T. | Kawazoe, M. | RSGI, RIKEN Structural Genomics/Proteomics Initiative. | Schluenzen, F. | Shirouzu, M. | Takemoto, C. | Wilson, D N. | Yokoyama, S. | ZN | 30s ribosomal subunit | 5' untranslated region | Mrna capture | National project on protein structural and functional analyse | Nppsfa | Riken structural genomics/proteomics initiative | Rsgi | Shine-dalgarno interaction | Structural genomic | Translation initiation
