2e5v

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[[Image:2e5v.jpg|left|200px]]<br /><applet load="2e5v" size="350" color="white" frame="true" align="right" spinBox="true"
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[[Image:2e5v.jpg|left|200px]]
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caption="2e5v, resolution 2.09&Aring;" />
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'''Crystal structure of L-Aspartate Oxidase from hyperthermophilic archaeon Sulfolobus tokodaii'''<br />
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{{Structure
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|PDB= 2e5v |SIZE=350|CAPTION= <scene name='initialview01'>2e5v</scene>, resolution 2.09&Aring;
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|SITE= <scene name='pdbsite=AC1:Cl+Binding+Site+For+Residue+A+2001'>AC1</scene>, <scene name='pdbsite=AC2:Cl+Binding+Site+For+Residue+B+2002'>AC2</scene>, <scene name='pdbsite=AC3:Fad+Binding+Site+For+Residue+B+1001'>AC3</scene> and <scene name='pdbsite=AC4:Fad+Binding+Site+For+Residue+A+1002'>AC4</scene>
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|LIGAND= <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene> and <scene name='pdbligand=FAD:FLAVIN-ADENINE DINUCLEOTIDE'>FAD</scene>
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|ACTIVITY= [http://en.wikipedia.org/wiki/L-aspartate_oxidase L-aspartate oxidase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.4.3.16 1.4.3.16]
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|GENE=
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}}
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'''Crystal structure of L-Aspartate Oxidase from hyperthermophilic archaeon Sulfolobus tokodaii'''
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==Overview==
==Overview==
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==About this Structure==
==About this Structure==
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2E5V is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Sulfolobus_tokodaii Sulfolobus tokodaii] with <scene name='pdbligand=CL:'>CL</scene> and <scene name='pdbligand=FAD:'>FAD</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/L-aspartate_oxidase L-aspartate oxidase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.4.3.16 1.4.3.16] Known structural/functional Sites: <scene name='pdbsite=AC1:Cl+Binding+Site+For+Residue+A+2001'>AC1</scene>, <scene name='pdbsite=AC2:Cl+Binding+Site+For+Residue+B+2002'>AC2</scene>, <scene name='pdbsite=AC3:Fad+Binding+Site+For+Residue+B+1001'>AC3</scene> and <scene name='pdbsite=AC4:Fad+Binding+Site+For+Residue+A+1002'>AC4</scene>. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2E5V OCA].
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2E5V is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Sulfolobus_tokodaii Sulfolobus tokodaii]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2E5V OCA].
==Reference==
==Reference==
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Structure of l-aspartate oxidase from the hyperthermophilic archaeon Sulfolobus tokodaii., Sakuraba H, Yoneda K, Asai I, Tsuge H, Katunuma N, Ohshima T, Biochim Biophys Acta. 2008 Mar;1784(3):563-71. Epub 2008 Jan 8. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=18226609 18226609]
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Structure of l-aspartate oxidase from the hyperthermophilic archaeon Sulfolobus tokodaii., Sakuraba H, Yoneda K, Asai I, Tsuge H, Katunuma N, Ohshima T, Biochim Biophys Acta. 2008 Mar;1784(3):563-71. Epub 2008 Jan 8. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/18226609 18226609]
[[Category: L-aspartate oxidase]]
[[Category: L-aspartate oxidase]]
[[Category: Single protein]]
[[Category: Single protein]]
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[[Category: oxidoreductase]]
[[Category: oxidoreductase]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Mar 5 13:25:20 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 16:35:50 2008''

Revision as of 14:35, 20 March 2008

Image:2e5v.jpg


PDB ID 2e5v

Drag the structure with the mouse to rotate
, resolution 2.09Å
Sites: , , and
Ligands: and
Activity: L-aspartate oxidase, with EC number 1.4.3.16
Coordinates: save as pdb, mmCIF, xml



Crystal structure of L-Aspartate Oxidase from hyperthermophilic archaeon Sulfolobus tokodaii


Overview

The crystal structure of the highly thermostable l-aspartate oxidase (LAO) from the hyperthermophilic archaeon Sulfolobus tokodaii was determined at a 2.09 A resolution. The factors contributing to the thermostability of the enzyme were analyzed by comparing its structure to that of Escherichia coli LAO. Like E. coli LAO, the S. tokodaii enzyme consists of three domains: an FAD-binding domain, an alpha+beta capping domain, and a C-terminal three-helix bundle. However, the situation of the linker between the FAD-binding domain and C-terminal three-helix bundle in S. tokodaii LAO is completely different from that in E. coli LAO, where the linker is situated near the FAD-binding domain and has virtually no interaction with the rest of the protein. In S. tokodaii LAO, this linker is situated near the C-terminal three-helix bundle and contains a beta-strand that runs parallel to the C-terminal strand. This results in the formation of an additional beta-sheet, which appears to reduce the flexibility of the C-terminal region. Furthermore, the displacement of the linker enables formation of a 5-residue ion-pair network between the FAD-binding and C-terminal domains, which strengthens the interdomain interactions. These features might be the main factors contributing to the high thermostability of S. tokodaii LAO.

About this Structure

2E5V is a Single protein structure of sequence from Sulfolobus tokodaii. Full crystallographic information is available from OCA.

Reference

Structure of l-aspartate oxidase from the hyperthermophilic archaeon Sulfolobus tokodaii., Sakuraba H, Yoneda K, Asai I, Tsuge H, Katunuma N, Ohshima T, Biochim Biophys Acta. 2008 Mar;1784(3):563-71. Epub 2008 Jan 8. PMID:18226609

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