4cxf
From Proteopedia
(Difference between revisions)
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== Structural highlights == | == Structural highlights == | ||
<table><tr><td colspan='2'>[[4cxf]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Cupriavidus_metallidurans_ch34 Cupriavidus metallidurans ch34]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4CXF OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4CXF FirstGlance]. <br> | <table><tr><td colspan='2'>[[4cxf]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Cupriavidus_metallidurans_ch34 Cupriavidus metallidurans ch34]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4CXF OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4CXF FirstGlance]. <br> | ||
| - | </td></tr><tr><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>< | + | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr> |
| - | <tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4cxf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4cxf OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4cxf RCSB], [http://www.ebi.ac.uk/pdbsum/4cxf PDBsum]</span></td></tr> | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4cxf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4cxf OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4cxf RCSB], [http://www.ebi.ac.uk/pdbsum/4cxf PDBsum]</span></td></tr> |
| - | <table> | + | </table> |
| + | == Function == | ||
| + | [[http://www.uniprot.org/uniprot/CNRH_RALME CNRH_RALME]] Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released. This sigma factor regulates the genes for a membrane-located efflux system that confers resistance to nickel and cobalt. CnrH alone is able to activate CNR expression, while both CnrY and CrnX are needed for nickel induction of cnrH (PubMed:10671463). Binds DNA in an RNA polymerase-dependent fashion (PubMed:10671464). CnrH may be controlled by a CnrYX transmembrane anti-sigma factor complex which binds CnrH in the absence of Ni(2+). If Ni(2+) appears in the periplasm, it may be bound by CnrR (CnrX); the signal then would be transmitted by CnrY into the cytoplasm and CnrH would be released. [[http://www.uniprot.org/uniprot/CNRY_RALME CNRY_RALME]] Nickel and cobalt resistance proteins CnrA, CnrB, CnrC CnrH and CnrR may be involved in the regulation of CNR. CnrH alone is able to activate cnr expression, and both CnrY and CrnX are needed for nickel induction of CnrH (PubMed:10671463). In the absence of wild-type CnrY (due either to a frameshift, PubMed:10671463 or absence of the transcript, PubMed:10671464), nickel and cobalt resistance is constitutive, indicating that CrnY may act as a repressor (PubMed:10671463) or an anti-sigma factor (PubMed:10671464). | ||
<div style="background-color:#fffaf0;"> | <div style="background-color:#fffaf0;"> | ||
== Publication Abstract from PubMed == | == Publication Abstract from PubMed == | ||
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The Crystal Structure of the Anti-sigma Factor CnrY in Complex with the sigma Factor CnrH Shows a New Structural Class of Anti-sigma Factors Targeting Extracytoplasmic Function sigma Factors.,Maillard AP, Girard E, Ziani W, Petit-Hartlein I, Kahn R, Coves J J Mol Biol. 2014 Apr 12. pii: S0022-2836(14)00177-6. doi:, 10.1016/j.jmb.2014.04.003. PMID:24727125<ref>PMID:24727125</ref> | The Crystal Structure of the Anti-sigma Factor CnrY in Complex with the sigma Factor CnrH Shows a New Structural Class of Anti-sigma Factors Targeting Extracytoplasmic Function sigma Factors.,Maillard AP, Girard E, Ziani W, Petit-Hartlein I, Kahn R, Coves J J Mol Biol. 2014 Apr 12. pii: S0022-2836(14)00177-6. doi:, 10.1016/j.jmb.2014.04.003. PMID:24727125<ref>PMID:24727125</ref> | ||
| - | From | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> |
</div> | </div> | ||
| + | |||
| + | ==See Also== | ||
| + | *[[Sigma factor|Sigma factor]] | ||
== References == | == References == | ||
<references/> | <references/> | ||
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</StructureSection> | </StructureSection> | ||
[[Category: Cupriavidus metallidurans ch34]] | [[Category: Cupriavidus metallidurans ch34]] | ||
| - | [[Category: Coves, J | + | [[Category: Coves, J]] |
| - | [[Category: Girard, E | + | [[Category: Girard, E]] |
| - | [[Category: Kahn, R | + | [[Category: Kahn, R]] |
| - | [[Category: Maillard, A P | + | [[Category: Maillard, A P]] |
| - | [[Category: Petit-Hartlein, I | + | [[Category: Petit-Hartlein, I]] |
| - | [[Category: Ziani, W | + | [[Category: Ziani, W]] |
[[Category: Antisigma]] | [[Category: Antisigma]] | ||
[[Category: Ecf-type sigma]] | [[Category: Ecf-type sigma]] | ||
[[Category: Transcription]] | [[Category: Transcription]] | ||
Revision as of 16:09, 25 December 2014
Structure of CnrH in complex with the cytosolic domain of CnrY.
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