2e7z

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[[Image:2e7z.jpg|left|200px]]<br /><applet load="2e7z" size="350" color="white" frame="true" align="right" spinBox="true"
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[[Image:2e7z.jpg|left|200px]]
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caption="2e7z, resolution 1.26&Aring;" />
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'''Acetylene Hydratase from Pelobacter acetylenicus'''<br />
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{{Structure
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|PDB= 2e7z |SIZE=350|CAPTION= <scene name='initialview01'>2e7z</scene>, resolution 1.26&Aring;
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|SITE=
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|LIGAND= <scene name='pdbligand=NA:SODIUM+ION'>NA</scene>, <scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene>, <scene name='pdbligand=SF4:IRON/SULFUR+CLUSTER'>SF4</scene>, <scene name='pdbligand=MGD:2-AMINO-5,6-DIMERCAPTO-7-METHYL-3,7,8A,9-TETRAHYDRO-8-OXA-1,3,9,10-TETRAAZA-ANTHRACEN-4-ONE+GUANOSINE+DINUCLEOTIDE'>MGD</scene>, <scene name='pdbligand=W:TUNGSTEN+ION'>W</scene> and <scene name='pdbligand=MPD:(4S)-2-METHYL-2,4-PENTANEDIOL'>MPD</scene>
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|ACTIVITY= [http://en.wikipedia.org/wiki/Deleted_entry Deleted entry], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=4.2.1.71 4.2.1.71]
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|GENE=
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}}
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'''Acetylene Hydratase from Pelobacter acetylenicus'''
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==Overview==
==Overview==
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==About this Structure==
==About this Structure==
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2E7Z is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Pelobacter_acetylenicus Pelobacter acetylenicus] with <scene name='pdbligand=NA:'>NA</scene>, <scene name='pdbligand=ACT:'>ACT</scene>, <scene name='pdbligand=SF4:'>SF4</scene>, <scene name='pdbligand=MGD:'>MGD</scene>, <scene name='pdbligand=W:'>W</scene> and <scene name='pdbligand=MPD:'>MPD</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Deleted_entry Deleted entry], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=4.2.1.71 4.2.1.71] Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2E7Z OCA].
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2E7Z is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Pelobacter_acetylenicus Pelobacter acetylenicus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2E7Z OCA].
==Reference==
==Reference==
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Structure of the non-redox-active tungsten/[4Fe:4S] enzyme acetylene hydratase., Seiffert GB, Ullmann GM, Messerschmidt A, Schink B, Kroneck PM, Einsle O, Proc Natl Acad Sci U S A. 2007 Feb 27;104(9):3073-7. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=17360611 17360611]
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Structure of the non-redox-active tungsten/[4Fe:4S] enzyme acetylene hydratase., Seiffert GB, Ullmann GM, Messerschmidt A, Schink B, Kroneck PM, Einsle O, Proc Natl Acad Sci U S A. 2007 Feb 27;104(9):3073-7. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/17360611 17360611]
[[Category: Deleted entry]]
[[Category: Deleted entry]]
[[Category: Pelobacter acetylenicus]]
[[Category: Pelobacter acetylenicus]]
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[[Category: tungstoprotein]]
[[Category: tungstoprotein]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 17:07:04 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 16:36:33 2008''

Revision as of 14:36, 20 March 2008

Image:2e7z.jpg


PDB ID 2e7z

Drag the structure with the mouse to rotate
, resolution 1.26Å
Ligands: , , , , and
Activity: Deleted entry, with EC number 4.2.1.71
Coordinates: save as pdb, mmCIF, xml



Acetylene Hydratase from Pelobacter acetylenicus


Overview

The tungsten-iron-sulfur enzyme acetylene hydratase stands out from its class because it catalyzes a nonredox reaction, the hydration of acetylene to acetaldehyde. Sequence comparisons group the protein into the dimethyl sulfoxide reductase family, and it contains a bis-molybdopterin guanine dinucleotide-ligated tungsten atom and a cubane-type [4Fe:4S] cluster. The crystal structure of acetylene hydratase at 1.26 A now shows that the tungsten center binds a water molecule that is activated by an adjacent aspartate residue, enabling it to attack acetylene bound in a distinct, hydrophobic pocket. This mechanism requires a strong shift of pK(a) of the aspartate, caused by a nearby low-potential [4Fe:4S] cluster. To access this previously unrecognized W-Asp active site, the protein evolved a new substrate channel distant from where it is found in other molybdenum and tungsten enzymes.

About this Structure

2E7Z is a Single protein structure of sequence from Pelobacter acetylenicus. Full crystallographic information is available from OCA.

Reference

Structure of the non-redox-active tungsten/[4Fe:4S] enzyme acetylene hydratase., Seiffert GB, Ullmann GM, Messerschmidt A, Schink B, Kroneck PM, Einsle O, Proc Natl Acad Sci U S A. 2007 Feb 27;104(9):3073-7. PMID:17360611

Page seeded by OCA on Thu Mar 20 16:36:33 2008

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