3j2u

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{{STRUCTURE_3j2u| PDB=3j2u | SCENE= }}
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==Kinesin-13 KLP10A HD in complex with CS-tubulin and a microtubule==
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===Kinesin-13 KLP10A HD in complex with CS-tubulin and a microtubule===
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<StructureSection load='3j2u' size='340' side='right' caption='[[3j2u]], [[Resolution|resolution]] 10.80&Aring;' scene=''>
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{{ABSTRACT_PUBMED_23434508}}
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== Structural highlights ==
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<table><tr><td colspan='2'>[[3j2u]] is a 5 chain structure with sequence from [http://en.wikipedia.org/wiki/Bos_taurus Bos taurus] and [http://en.wikipedia.org/wiki/Gekko_coi Gekko coi]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3J2U OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3J2U FirstGlance]. <br>
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==Function==
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</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">Klp10A, CG1453 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1118509 Gekko coi])</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3j2u FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3j2u OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3j2u RCSB], [http://www.ebi.ac.uk/pdbsum/3j2u PDBsum]</span></td></tr>
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</table>
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== Function ==
[[http://www.uniprot.org/uniprot/KI10A_DROME KI10A_DROME]] Required during anaphase to drive sister chromatid separation to promote flux by actively depolymerizing kinetochore microtubules at their pole-associated minus ends, thereby moving chromatids through a "poleward flux".<ref>PMID:14681690</ref> [[http://www.uniprot.org/uniprot/TBB2B_BOVIN TBB2B_BOVIN]] Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain (By similarity). [[http://www.uniprot.org/uniprot/TBA1B_BOVIN TBA1B_BOVIN]] Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain.
[[http://www.uniprot.org/uniprot/KI10A_DROME KI10A_DROME]] Required during anaphase to drive sister chromatid separation to promote flux by actively depolymerizing kinetochore microtubules at their pole-associated minus ends, thereby moving chromatids through a "poleward flux".<ref>PMID:14681690</ref> [[http://www.uniprot.org/uniprot/TBB2B_BOVIN TBB2B_BOVIN]] Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain (By similarity). [[http://www.uniprot.org/uniprot/TBA1B_BOVIN TBA1B_BOVIN]] Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain.
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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To elucidate the structural basis of the mechanism of microtubule depolymerization by kinesin-13s, we analyzed complexes of tubulin and the Drosophila melanogaster kinesin-13 KLP10A by electron microscopy (EM) and fluorescence polarization microscopy. We report a nanometer-resolution (1.1 nm) cryo-EM three-dimensional structure of the KLP10A head domain (KLP10AHD) bound to curved tubulin. We found that binding of KLP10AHD induces a distinct tubulin configuration with displacement (shear) between tubulin subunits in addition to curvature. In this configuration, the kinesin-binding site differs from that in straight tubulin, providing an explanation for the distinct interaction modes of kinesin-13s with the microtubule lattice or its ends. The KLP10AHD-tubulin interface comprises three areas of interaction, suggesting a crossbow-type tubulin-bending mechanism. These areas include the kinesin-13 family conserved KVD residues, and as predicted from the crossbow model, mutating these residues changes the orientation and mobility of KLP10AHDs interacting with the microtubule.
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Structural Model for Tubulin Recognition and Deformation by Kinesin-13 Microtubule Depolymerases.,Asenjo AB, Chatterjee C, Tan D, Depaoli V, Rice WJ, Diaz-Avalos R, Silvestry M, Sosa H Cell Rep. 2013 Feb 19. pii: S2211-1247(13)00054-5. doi:, 10.1016/j.celrep.2013.01.030. PMID:23434508<ref>PMID:23434508</ref>
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==About this Structure==
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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[[3j2u]] is a 5 chain structure with sequence from [http://en.wikipedia.org/wiki/Bos_taurus Bos taurus] and [http://en.wikipedia.org/wiki/Gekko_coi Gekko coi]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3J2U OCA].
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</div>
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==Reference==
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==See Also==
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<references group="xtra"/><references/>
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*[[Tubulin|Tubulin]]
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== References ==
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<references/>
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__TOC__
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</StructureSection>
[[Category: Bos taurus]]
[[Category: Bos taurus]]
[[Category: Gekko coi]]
[[Category: Gekko coi]]
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[[Category: Asenjo, A B.]]
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[[Category: Asenjo, A B]]
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[[Category: Chatterjee, C.]]
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[[Category: Chatterjee, C]]
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[[Category: DePaoli, V.]]
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[[Category: DePaoli, V]]
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[[Category: Diaz-Avalos, R.]]
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[[Category: Diaz-Avalos, R]]
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[[Category: Rice, W J.]]
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[[Category: Rice, W J]]
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[[Category: Silvestry, M.]]
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[[Category: Silvestry, M]]
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[[Category: Sosa, H.]]
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[[Category: Sosa, H]]
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[[Category: Tan, D.]]
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[[Category: Tan, D]]
[[Category: Depolymerase]]
[[Category: Depolymerase]]
[[Category: Depolymerization]]
[[Category: Depolymerization]]

Revision as of 17:47, 25 December 2014

Kinesin-13 KLP10A HD in complex with CS-tubulin and a microtubule

3j2u, resolution 10.80Å

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