2ek5
From Proteopedia
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| - | [[Image:2ek5.jpg|left|200px]] | + | [[Image:2ek5.jpg|left|200px]] |
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| - | '''Crystal strucutre of the transcriptional factor from C.glutamicum at 2.2 angstrom resolution''' | + | {{Structure |
| + | |PDB= 2ek5 |SIZE=350|CAPTION= <scene name='initialview01'>2ek5</scene>, resolution 2.2Å | ||
| + | |SITE= | ||
| + | |LIGAND= | ||
| + | |ACTIVITY= | ||
| + | |GENE= cgl2947 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1718 Corynebacterium glutamicum]) | ||
| + | }} | ||
| + | |||
| + | '''Crystal strucutre of the transcriptional factor from C.glutamicum at 2.2 angstrom resolution''' | ||
| + | |||
==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
| - | 2EK5 is a [ | + | 2EK5 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Corynebacterium_glutamicum Corynebacterium glutamicum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2EK5 OCA]. |
==Reference== | ==Reference== | ||
| - | The structures of transcription factor CGL2947 from Corynebacterium glutamicum in two crystal forms: a novel homodimer assembling and the implication for effector-binding mode., Gao YG, Yao M, Itou H, Zhou Y, Tanaka I, Protein Sci. 2007 Sep;16(9):1878-86. PMID:[http:// | + | The structures of transcription factor CGL2947 from Corynebacterium glutamicum in two crystal forms: a novel homodimer assembling and the implication for effector-binding mode., Gao YG, Yao M, Itou H, Zhou Y, Tanaka I, Protein Sci. 2007 Sep;16(9):1878-86. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/17766384 17766384] |
[[Category: Corynebacterium glutamicum]] | [[Category: Corynebacterium glutamicum]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
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[[Category: Yao, M.]] | [[Category: Yao, M.]] | ||
[[Category: helix-turn-helix]] | [[Category: helix-turn-helix]] | ||
| - | [[Category: interwined alpha | + | [[Category: interwined alpha helice]] |
[[Category: transcription]] | [[Category: transcription]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 16:41:19 2008'' |
Revision as of 14:41, 20 March 2008
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| , resolution 2.2Å | |||||||
|---|---|---|---|---|---|---|---|
| Gene: | cgl2947 (Corynebacterium glutamicum) | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
Crystal strucutre of the transcriptional factor from C.glutamicum at 2.2 angstrom resolution
Overview
Among the transcription factors, the helix-turn-helix (HTH) GntR family comprised of FadR, HutC, MocR, YtrA, AraR, and PlmA subfamilies regulates the most varied biological processes. Generally, proteins belonging to this family contain an N-terminal DNA-binding domain and a C-terminal effector-binding/oligomerization domain. The members of the YtrA subfamily are much shorter than other members of this family, with chain lengths of 120-130 residues with about 50 residues located in the C-terminal domain. Because of this length, the mode of dimerization and the ability to bind effectors by the C-terminal domain are puzzling. Here, we first report the structure of the transcription factor CGL2947 from Corynebacterium glutamicum, which belongs to the YtrA family. The monomer is composed of a DNA-binding domain containing a winged HTH motif in the N terminus and two helices (alpha4 and alpha5) with a fishhook-shaped arrangement in the C terminus. Helices alpha4 and alpha5 of two monomers intertwine together to form a novel homodimer assembly. The effector-accommodating pocket with 2-methyl-2,4-pentanediol (MPD) docked was located, and it was suggested to represent a novel mode of effector binding. The structures in two crystal forms (MPD-free and -bound in the proposed effector-binding pocket) were solved. The structural variations have implications regarding how the effector-induced conformational change modulates DNA affinity for YtrA family members.
About this Structure
2EK5 is a Single protein structure of sequence from Corynebacterium glutamicum. Full crystallographic information is available from OCA.
Reference
The structures of transcription factor CGL2947 from Corynebacterium glutamicum in two crystal forms: a novel homodimer assembling and the implication for effector-binding mode., Gao YG, Yao M, Itou H, Zhou Y, Tanaka I, Protein Sci. 2007 Sep;16(9):1878-86. PMID:17766384
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