4n0g
From Proteopedia
(Difference between revisions)
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| - | + | ==Crystal Structure of PYL13-PP2CA complex== | |
| - | === | + | <StructureSection load='4n0g' size='340' side='right' caption='[[4n0g]], [[Resolution|resolution]] 2.38Å' scene=''> |
| - | + | == Structural highlights == | |
| - | + | <table><tr><td colspan='2'>[[4n0g]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Arath Arath]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4N0G OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4N0G FirstGlance]. <br> | |
| - | ==Function== | + | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr> |
| + | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">PP2CA, AHG3, At3g11410, F24K9.8 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=3702 ARATH]), PYL13, RCAR7, At4g18620, F28A21.30 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=3702 ARATH])</td></tr> | ||
| + | <tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Phosphoprotein_phosphatase Phosphoprotein phosphatase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.3.16 3.1.3.16] </span></td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4n0g FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4n0g OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4n0g RCSB], [http://www.ebi.ac.uk/pdbsum/4n0g PDBsum]</span></td></tr> | ||
| + | </table> | ||
| + | == Function == | ||
[[http://www.uniprot.org/uniprot/P2C37_ARATH P2C37_ARATH]] Major negative regulator of abscisic acid (ABA) responses during seed germination and cold acclimation. Confers insensitivity to ABA. Modulates negatively the AKT2/3 activity, which mediates K(+) transport and membrane polarization during stress situations, probably by dephosphorylation. Prevent stomata closure by inactivating the S-type anion efflux channel SLAC1 and its activator SRK2E.<ref>PMID:9448270</ref> <ref>PMID:11439132</ref> <ref>PMID:12034902</ref> <ref>PMID:16339800</ref> <ref>PMID:16361522</ref> <ref>PMID:17461784</ref> <ref>PMID:19955427</ref> [[http://www.uniprot.org/uniprot/PYL13_ARATH PYL13_ARATH]] Receptor for abscisic acid (ABA) required for ABA-mediated responses such as stomatal closure and germination inhibition. Inhibits the activity of group-A protein phosphatases type 2C (PP2Cs) when activated by ABA (By similarity). | [[http://www.uniprot.org/uniprot/P2C37_ARATH P2C37_ARATH]] Major negative regulator of abscisic acid (ABA) responses during seed germination and cold acclimation. Confers insensitivity to ABA. Modulates negatively the AKT2/3 activity, which mediates K(+) transport and membrane polarization during stress situations, probably by dephosphorylation. Prevent stomata closure by inactivating the S-type anion efflux channel SLAC1 and its activator SRK2E.<ref>PMID:9448270</ref> <ref>PMID:11439132</ref> <ref>PMID:12034902</ref> <ref>PMID:16339800</ref> <ref>PMID:16361522</ref> <ref>PMID:17461784</ref> <ref>PMID:19955427</ref> [[http://www.uniprot.org/uniprot/PYL13_ARATH PYL13_ARATH]] Receptor for abscisic acid (ABA) required for ABA-mediated responses such as stomatal closure and germination inhibition. Inhibits the activity of group-A protein phosphatases type 2C (PP2Cs) when activated by ABA (By similarity). | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | PYR1/PYL/RCAR family proteins (PYLs) are well-characterized abscisic acid (ABA) receptors. Among the 14 PYL members in Arabidopsis thaliana, PYL13 is ABA irresponsive and its function has remained elusive. Here, we show that PYL13 selectively inhibits the phosphatase activity of PP2CA independent of ABA. The crystal structure of PYL13-PP2CA complex, which was determined at 2.4 A resolution, elucidates the molecular basis for the specific recognition between PP2CA and PYL13. In addition to the canonical interactions between PYLs and PP2Cs, an extra interface is identified involving an element in the vicinity of a previously uncharacterized CCCH zinc-finger (ZF) motif in PP2CA. Sequence blast identified another 56 ZF-containing PP2Cs, all of which are from plants. The structure also reveals the molecular determinants for the ABA irresponsiveness of PYL13. Finally, biochemical analysis suggests that PYL13 may hetero-oligomerize with PYL10. These two PYLs antagonize each other in their respective ABA-independent inhibitions of PP2Cs. The biochemical and structural studies provide important insights into the function of PYL13 in the stress response of plant and set up a foundation for future biotechnological applications of PYL13.Cell Research advance online publication 29 October 2013; doi:10.1038/cr.2013.143. | ||
| + | |||
| + | Molecular basis for the selective and ABA-independent inhibition of PP2CA by PYL13.,Li W, Wang L, Sheng X, Yan C, Zhou R, Hang J, Yin P, Yan N Cell Res. 2013 Oct 29. doi: 10.1038/cr.2013.143. PMID:24165892<ref>PMID:24165892</ref> | ||
| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| - | + | </div> | |
| - | == | + | ==See Also== |
| - | + | *[[Protein phosphatase|Protein phosphatase]] | |
| + | == References == | ||
| + | <references/> | ||
| + | __TOC__ | ||
| + | </StructureSection> | ||
[[Category: Arath]] | [[Category: Arath]] | ||
[[Category: Phosphoprotein phosphatase]] | [[Category: Phosphoprotein phosphatase]] | ||
| - | [[Category: Hang, J | + | [[Category: Hang, J]] |
| - | [[Category: Li, W | + | [[Category: Li, W]] |
| - | [[Category: Sheng, X | + | [[Category: Sheng, X]] |
| - | [[Category: Wang, L | + | [[Category: Wang, L]] |
| - | [[Category: Yan, C | + | [[Category: Yan, C]] |
| - | [[Category: Yan, N | + | [[Category: Yan, N]] |
| - | [[Category: Yin, P | + | [[Category: Yin, P]] |
| - | [[Category: Zhou, R | + | [[Category: Zhou, R]] |
[[Category: Aba receptor/phosphatase]] | [[Category: Aba receptor/phosphatase]] | ||
[[Category: Hydrolase-receptor complex]] | [[Category: Hydrolase-receptor complex]] | ||
Revision as of 17:49, 25 December 2014
Crystal Structure of PYL13-PP2CA complex
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