2erb

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[[Image:2erb.gif|left|200px]]<br /><applet load="2erb" size="350" color="white" frame="true" align="right" spinBox="true"
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[[Image:2erb.gif|left|200px]]
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caption="2erb, resolution 1.50&Aring;" />
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'''AgamOBP1, and odorant binding protein from Anopheles gambiae complexed with PEG'''<br />
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{{Structure
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|PDB= 2erb |SIZE=350|CAPTION= <scene name='initialview01'>2erb</scene>, resolution 1.50&Aring;
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|SITE=
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|LIGAND= <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene> and <scene name='pdbligand=PEU:2,5,8,11,14,17,20,23,26,29,32,35,38,41,44,47,50,53,56,59,62,65,68,71,74,77,80-HEPTACOSAOXADOOCTACONTAN-82-OL'>PEU</scene>
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|ACTIVITY=
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|GENE=
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}}
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'''AgamOBP1, and odorant binding protein from Anopheles gambiae complexed with PEG'''
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==Overview==
==Overview==
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==About this Structure==
==About this Structure==
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2ERB is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Anopheles_gambiae Anopheles gambiae] with <scene name='pdbligand=MG:'>MG</scene> and <scene name='pdbligand=PEU:'>PEU</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2ERB OCA].
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2ERB is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Anopheles_gambiae Anopheles gambiae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2ERB OCA].
==Reference==
==Reference==
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The crystal structure of an odorant binding protein from Anopheles gambiae: evidence for a common ligand release mechanism., Wogulis M, Morgan T, Ishida Y, Leal WS, Wilson DK, Biochem Biophys Res Commun. 2006 Jan 6;339(1):157-64. Epub 2005 Nov 9. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=16300742 16300742]
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The crystal structure of an odorant binding protein from Anopheles gambiae: evidence for a common ligand release mechanism., Wogulis M, Morgan T, Ishida Y, Leal WS, Wilson DK, Biochem Biophys Res Commun. 2006 Jan 6;339(1):157-64. Epub 2005 Nov 9. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/16300742 16300742]
[[Category: Anopheles gambiae]]
[[Category: Anopheles gambiae]]
[[Category: Single protein]]
[[Category: Single protein]]
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[[Category: MG]]
[[Category: MG]]
[[Category: PEU]]
[[Category: PEU]]
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[[Category: disulfides]]
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[[Category: disulfide]]
[[Category: helix]]
[[Category: helix]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 17:13:46 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 16:43:29 2008''

Revision as of 14:43, 20 March 2008

Image:2erb.gif


PDB ID 2erb

Drag the structure with the mouse to rotate
, resolution 1.50Å
Ligands: and
Coordinates: save as pdb, mmCIF, xml



AgamOBP1, and odorant binding protein from Anopheles gambiae complexed with PEG


Overview

The Anopheles gambiae mosquito is the main vector of malaria transmission in sub-Saharan Africa. We present here a 1.5A crystal structure of AgamOBP1, an odorant binding protein (OBP) from the A. gambiae mosquito. The protein crystallized as a dimer with a unique binding pocket consisting of a continuous tunnel running through both subunits of the dimer and occupied by a PEG molecule. We demonstrate that AgamOBP1 undergoes a pH dependent conformational change that is associated with reduced ligand binding. A predominance of acid-labile hydrogen bonds involving the C-terminal loop suggests a mechanism in which a drop in pH causes C-terminal loop to open, leaving the binding tunnel solvent exposed, thereby lowering binding affinity for ligand. Because proteins from two distantly related insects also undergo a pH dependent conformational change involving the C-terminus that is associated with reduced ligand affinity, our results suggest a common mechanism for OBP activity.

About this Structure

2ERB is a Single protein structure of sequence from Anopheles gambiae. Full crystallographic information is available from OCA.

Reference

The crystal structure of an odorant binding protein from Anopheles gambiae: evidence for a common ligand release mechanism., Wogulis M, Morgan T, Ishida Y, Leal WS, Wilson DK, Biochem Biophys Res Commun. 2006 Jan 6;339(1):157-64. Epub 2005 Nov 9. PMID:16300742

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