2crx
From Proteopedia
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==Overview== | ==Overview== | ||
| - | We have determined the X-ray crystal structures of two DNA Holliday, junctions (HJs) bound by Cre recombinase. The HJ is a four-way branched, structure that occurs as an intermediate in genetic recombination, pathways, including site-specific recombination by the lambda-integrase, family. Cre recombinase is an integrase family member that recombines 34, bp loxP sites in the absence of accessory proteins or auxiliary DNA, sequences. The 2.7 A structure of Cre recombinase bound to an immobile HJ, and the 2.5 A structure of Cre recombinase bound to a symmetric, nicked HJ, reveal a nearly planar, twofold-symmetric DNA intermediate that shares, features with both the stacked-X and the square conformations of the HJ, that exist in the unbound state. The structures support a protein-mediated, .. | + | We have determined the X-ray crystal structures of two DNA Holliday, junctions (HJs) bound by Cre recombinase. The HJ is a four-way branched, structure that occurs as an intermediate in genetic recombination, pathways, including site-specific recombination by the lambda-integrase, family. Cre recombinase is an integrase family member that recombines 34, bp loxP sites in the absence of accessory proteins or auxiliary DNA, sequences. The 2.7 A structure of Cre recombinase bound to an immobile HJ, and the 2.5 A structure of Cre recombinase bound to a symmetric, nicked HJ, reveal a nearly planar, twofold-symmetric DNA intermediate that shares, features with both the stacked-X and the square conformations of the HJ, that exist in the unbound state. The structures support a protein-mediated, crossover isomerization of the junction that acts as the switch, responsible for activation and deactivation of recombinase active sites., In this model, a subtle isomerization of the Cre recombinase-HJ quaternary, structure dictates which strands are cleaved during resolution of the, junction via a mechanism that involves neither branch migration nor, helical restacking. |
==About this Structure== | ==About this Structure== | ||
| - | 2CRX is a | + | 2CRX is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Bacteriophage_p1 Bacteriophage p1]. Structure known Active Sites: CIA and CIB. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=2CRX OCA]. |
==Reference== | ==Reference== | ||
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[[Category: recombination]] | [[Category: recombination]] | ||
| - | ''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Mon Nov 5 13:40:05 2007'' |
Revision as of 11:34, 5 November 2007
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STRUCTURE OF THE HOLLIDAY JUNCTION INTERMEDIATE IN CRE-LOXP SITE-SPECIFIC RECOMBINATION
Overview
We have determined the X-ray crystal structures of two DNA Holliday, junctions (HJs) bound by Cre recombinase. The HJ is a four-way branched, structure that occurs as an intermediate in genetic recombination, pathways, including site-specific recombination by the lambda-integrase, family. Cre recombinase is an integrase family member that recombines 34, bp loxP sites in the absence of accessory proteins or auxiliary DNA, sequences. The 2.7 A structure of Cre recombinase bound to an immobile HJ, and the 2.5 A structure of Cre recombinase bound to a symmetric, nicked HJ, reveal a nearly planar, twofold-symmetric DNA intermediate that shares, features with both the stacked-X and the square conformations of the HJ, that exist in the unbound state. The structures support a protein-mediated, crossover isomerization of the junction that acts as the switch, responsible for activation and deactivation of recombinase active sites., In this model, a subtle isomerization of the Cre recombinase-HJ quaternary, structure dictates which strands are cleaved during resolution of the, junction via a mechanism that involves neither branch migration nor, helical restacking.
About this Structure
2CRX is a Single protein structure of sequence from Bacteriophage p1. Structure known Active Sites: CIA and CIB. Full crystallographic information is available from OCA.
Reference
Structure of the Holliday junction intermediate in Cre-loxP site-specific recombination., Gopaul DN, Guo F, Van Duyne GD, EMBO J. 1998 Jul 15;17(14):4175-87. PMID:9670032
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