2euw
From Proteopedia
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| - | [[Image:2euw.gif|left|200px]] | + | [[Image:2euw.gif|left|200px]] |
| - | + | ||
| - | '''Structure of a Ndt80-DNA complex (MSE mutant mA4T)''' | + | {{Structure |
| + | |PDB= 2euw |SIZE=350|CAPTION= <scene name='initialview01'>2euw</scene>, resolution 1.680Å | ||
| + | |SITE= | ||
| + | |LIGAND= | ||
| + | |ACTIVITY= | ||
| + | |GENE= NDT80 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=4932 Saccharomyces cerevisiae]) | ||
| + | }} | ||
| + | |||
| + | '''Structure of a Ndt80-DNA complex (MSE mutant mA4T)''' | ||
| + | |||
==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
| - | 2EUW is a [ | + | 2EUW is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2EUW OCA]. |
==Reference== | ==Reference== | ||
| - | Principles of protein-DNA recognition revealed in the structural analysis of Ndt80-MSE DNA complexes., Lamoureux JS, Glover JN, Structure. 2006 Mar;14(3):555-65. PMID:[http:// | + | Principles of protein-DNA recognition revealed in the structural analysis of Ndt80-MSE DNA complexes., Lamoureux JS, Glover JN, Structure. 2006 Mar;14(3):555-65. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/16531239 16531239] |
[[Category: Saccharomyces cerevisiae]] | [[Category: Saccharomyces cerevisiae]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
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[[Category: ig-fold transcription factor]] | [[Category: ig-fold transcription factor]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 16:44:41 2008'' |
Revision as of 14:44, 20 March 2008
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| , resolution 1.680Å | |||||||
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| Gene: | NDT80 (Saccharomyces cerevisiae) | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
Structure of a Ndt80-DNA complex (MSE mutant mA4T)
Overview
The Saccharomyces cerevisiae transcription factor Ndt80 selectively binds a DNA consensus sequence (the middle sporulation element [MSE]) to activate gene expression after the successful completion of meiotic recombination. Here we report the X-ray crystal structures of Ndt80 bound to ten distinct MSE variants. Comparison of these structures with the structure of Ndt80 bound to a consensus MSE reveals structural principles that determine the DNA binding specificity of this transcription factor. The 5' GC-rich end of the MSE contains distinct 5'-YpG-3' steps that are recognized by arginine side chains through a combination of hydrogen bonding and cation-pi interactions. The 3' AT-rich region is recognized via minor groove contacts that sterically exclude the N2 atom of GC base pairs. The conformation of the AT-rich region is fixed by interactions with the protein that favor recognition of poly(A)-poly(T) versus mixed AT sequences through an avoidance of major groove steric clashes at 5'-ApT-3' steps.
About this Structure
2EUW is a Single protein structure of sequence from Saccharomyces cerevisiae. Full crystallographic information is available from OCA.
Reference
Principles of protein-DNA recognition revealed in the structural analysis of Ndt80-MSE DNA complexes., Lamoureux JS, Glover JN, Structure. 2006 Mar;14(3):555-65. PMID:16531239
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