2exv
From Proteopedia
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| - | [[Image:2exv.gif|left|200px]] | + | [[Image:2exv.gif|left|200px]] |
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| - | '''Crystal structure of the F7A mutant of the cytochrome c551 from Pseudomonas aeruginosa''' | + | {{Structure |
| + | |PDB= 2exv |SIZE=350|CAPTION= <scene name='initialview01'>2exv</scene>, resolution 1.86Å | ||
| + | |SITE= | ||
| + | |LIGAND= <scene name='pdbligand=HEC:HEME+C'>HEC</scene> and <scene name='pdbligand=ACY:ACETIC ACID'>ACY</scene> | ||
| + | |ACTIVITY= | ||
| + | |GENE= nirM ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=287 Pseudomonas aeruginosa]) | ||
| + | }} | ||
| + | |||
| + | '''Crystal structure of the F7A mutant of the cytochrome c551 from Pseudomonas aeruginosa''' | ||
| + | |||
==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
| - | 2EXV is a [ | + | 2EXV is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Pseudomonas_aeruginosa Pseudomonas aeruginosa]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2EXV OCA]. |
==Reference== | ==Reference== | ||
| - | Unveiling a hidden folding intermediate in c-type cytochromes by protein engineering., Borgia A, Bonivento D, Travaglini-Allocatelli C, Di Matteo A, Brunori M, J Biol Chem. 2006 Apr 7;281(14):9331-6. Epub 2006 Feb 1. PMID:[http:// | + | Unveiling a hidden folding intermediate in c-type cytochromes by protein engineering., Borgia A, Bonivento D, Travaglini-Allocatelli C, Di Matteo A, Brunori M, J Biol Chem. 2006 Apr 7;281(14):9331-6. Epub 2006 Feb 1. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/16452476 16452476] |
[[Category: Pseudomonas aeruginosa]] | [[Category: Pseudomonas aeruginosa]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
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[[Category: heme c]] | [[Category: heme c]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 16:45:42 2008'' |
Revision as of 14:45, 20 March 2008
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| , resolution 1.86Å | |||||||
|---|---|---|---|---|---|---|---|
| Ligands: | and | ||||||
| Gene: | nirM (Pseudomonas aeruginosa) | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
Crystal structure of the F7A mutant of the cytochrome c551 from Pseudomonas aeruginosa
Overview
Several investigators have highlighted a correlation between the basic features of the folding process of a protein and its topology, which dictates the folding pathway. Within this conceptual framework we proposed that different members of the cytochrome c (cyt c) family share the same folding mechanism, involving a consensus partially structured state. Pseudomonas aeruginosa cyt c(551) (Pa cyt c(551)) folds via an apparent two-state mechanism through a high energy intermediate. Here we present kinetic evidence demonstrating that it is possible to switch its folding mechanism from two to three state, stabilizing the high energy intermediate by rational mutagenesis. Characterization of the folding kinetics of one single-site mutant of the Pa cyt c(551) (Phe(7) to Ala) indeed reveals an additional refolding phase and a fast unfolding process which are explained by the accumulation of a partially folded species. Further kinetic analysis highlights the presence of two parallel processes both leading to the native state, suggesting that the above mentioned species is a non obligatory on-pathway intermediate. Determination of the crystallographic structure of F7A shows the presence of an extended internal cavity, which hosts three "bound" water molecules and a H-bond in the N-terminal helix, which is shorter than in the wild type protein. These two features allow us to propose a detailed structural interpretation for the stabilization of the native and especially the intermediate states induced by a single crucial mutation. These results show how protein engineering, x-ray crystallography and state-of-the-art kinetics concur to unveil a folding intermediate and the structural determinants of its stability.
About this Structure
2EXV is a Single protein structure of sequence from Pseudomonas aeruginosa. Full crystallographic information is available from OCA.
Reference
Unveiling a hidden folding intermediate in c-type cytochromes by protein engineering., Borgia A, Bonivento D, Travaglini-Allocatelli C, Di Matteo A, Brunori M, J Biol Chem. 2006 Apr 7;281(14):9331-6. Epub 2006 Feb 1. PMID:16452476
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