2f2h

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[[Image:2f2h.gif|left|200px]]<br /><applet load="2f2h" size="350" color="white" frame="true" align="right" spinBox="true"
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[[Image:2f2h.gif|left|200px]]
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caption="2f2h, resolution 1.950&Aring;" />
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'''Structure of the YicI thiosugar Michaelis complex'''<br />
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{{Structure
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|PDB= 2f2h |SIZE=350|CAPTION= <scene name='initialview01'>2f2h</scene>, resolution 1.950&Aring;
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|SITE=
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|LIGAND= <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>, <scene name='pdbligand=MPO:3[N-MORPHOLINO]PROPANE+SULFONIC+ACID'>MPO</scene>, <scene name='pdbligand=XTG:4-NITROPHENYL+6-THIO-6-S-ALPHA-D-XYLOPYRANOSYL-BETA-D-GLUCOPYRANOSIDE'>XTG</scene> and <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>
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|ACTIVITY=
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|GENE= yicI ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=562 Escherichia coli])
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}}
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'''Structure of the YicI thiosugar Michaelis complex'''
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==Overview==
==Overview==
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==About this Structure==
==About this Structure==
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2F2H is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli] with <scene name='pdbligand=SO4:'>SO4</scene>, <scene name='pdbligand=MPO:'>MPO</scene>, <scene name='pdbligand=XTG:'>XTG</scene> and <scene name='pdbligand=GOL:'>GOL</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2F2H OCA].
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2F2H is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2F2H OCA].
==Reference==
==Reference==
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Expanding the thioglycoligase strategy to the synthesis of alpha-linked thioglycosides allows structural investigation of the parent enzyme/substrate complex., Kim YW, Lovering AL, Chen H, Kantner T, McIntosh LP, Strynadka NC, Withers SG, J Am Chem Soc. 2006 Feb 22;128(7):2202-3. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=16478160 16478160]
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Expanding the thioglycoligase strategy to the synthesis of alpha-linked thioglycosides allows structural investigation of the parent enzyme/substrate complex., Kim YW, Lovering AL, Chen H, Kantner T, McIntosh LP, Strynadka NC, Withers SG, J Am Chem Soc. 2006 Feb 22;128(7):2202-3. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/16478160 16478160]
[[Category: Escherichia coli]]
[[Category: Escherichia coli]]
[[Category: Single protein]]
[[Category: Single protein]]
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[[Category: beta8alpha8 barrel]]
[[Category: beta8alpha8 barrel]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 17:17:01 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 16:47:18 2008''

Revision as of 14:47, 20 March 2008

Image:2f2h.gif


PDB ID 2f2h

Drag the structure with the mouse to rotate
, resolution 1.950Å
Ligands: , , and
Gene: yicI (Escherichia coli)
Coordinates: save as pdb, mmCIF, xml



Structure of the YicI thiosugar Michaelis complex


Overview

For the first time, the thioglycoligase strategy has been successfully applied to alpha-glycosidases. The alpha-thioglycoligases derived from the family 31 glycosidases, alpha-xylosidase from E. coli (YicI) and alpha-glucosidase from Sulfolobus solfataricus, catalyze thioglycoligase reactions using alpha-glycosyl fluorides and deoxythioglycosides as donors and acceptors, respectively, in yields up to 86%. In addition, we describe the Michaelis complex of YicI using one of the thioglycosides as a nonhydrolyzable substrate analogue and discuss the structural insights this yields into the specificity and mechanism of family 31 alpha-glycosidases and the molecular basis of an associated genetic disease.

About this Structure

2F2H is a Single protein structure of sequence from Escherichia coli. Full crystallographic information is available from OCA.

Reference

Expanding the thioglycoligase strategy to the synthesis of alpha-linked thioglycosides allows structural investigation of the parent enzyme/substrate complex., Kim YW, Lovering AL, Chen H, Kantner T, McIntosh LP, Strynadka NC, Withers SG, J Am Chem Soc. 2006 Feb 22;128(7):2202-3. PMID:16478160

Page seeded by OCA on Thu Mar 20 16:47:18 2008

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