3vr1
From Proteopedia
(Difference between revisions)
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| - | + | ==Crystal structure analysis of the translation factor RF3== | |
| - | + | <StructureSection load='3vr1' size='340' side='right' caption='[[3vr1]], [[Resolution|resolution]] 3.00Å' scene=''> | |
| - | + | == Structural highlights == | |
| - | + | <table><tr><td colspan='2'>[[3vr1]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Desulfovibrio_vulgaris_str._'miyazaki_f' Desulfovibrio vulgaris str. 'miyazaki f']. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3VR1 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3VR1 FirstGlance]. <br> | |
| - | ==Function== | + | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=G4P:GUANOSINE-5,3-TETRAPHOSPHATE'>G4P</scene></td></tr> |
| + | <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[3vqt|3vqt]]</td></tr> | ||
| + | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">DvMF_2372, prfC ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=883 Desulfovibrio vulgaris str. 'Miyazaki F'])</td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3vr1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3vr1 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3vr1 RCSB], [http://www.ebi.ac.uk/pdbsum/3vr1 PDBsum]</span></td></tr> | ||
| + | </table> | ||
| + | == Function == | ||
[[http://www.uniprot.org/uniprot/RF3_DESVM RF3_DESVM]] Increases the formation of ribosomal termination complexes and stimulates activities of RF-1 and RF-2. It binds guanine nucleotides and has strong preference for UGA stop codons. It may interact directly with the ribosome. The stimulation of RF-1 and RF-2 is significantly reduced by GTP and GDP, but not by GMP (By similarity). | [[http://www.uniprot.org/uniprot/RF3_DESVM RF3_DESVM]] Increases the formation of ribosomal termination complexes and stimulates activities of RF-1 and RF-2. It binds guanine nucleotides and has strong preference for UGA stop codons. It may interact directly with the ribosome. The stimulation of RF-1 and RF-2 is significantly reduced by GTP and GDP, but not by GMP (By similarity). | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | The bacterial translational GTPases release factor RF3 promotes translation termination by recycling RF1 or RF2. Here, we present the crystal structures of RF3 complexed with GDP and guanosine 3',5'-(bis)diphosphate (ppGpp) at resolutions of 1.8 and 3.0A, respectively. ppGpp is involved in the so-called "stringent response" of bacteria. ppGpp binds at the same site as GDP, suggesting that GDP and ppGpp are two alternative physiologically relevant ligands of RF3. We also found that ppGpp decelerates the recycling of RF1 by RF3. These lines of evidence suggest that RF3 functions both as a cellular metabolic sensor and as a regulator. | ||
| - | + | Crystal structure analysis of the translation factor RF3 (release factor 3).,Kihira K, Shimizu Y, Shomura Y, Shibata N, Kitamura M, Nakagawa A, Ueda T, Ochi K, Higuchi Y FEBS Lett. 2012 Oct 19;586(20):3705-9. doi: 10.1016/j.febslet.2012.08.029. Epub, 2012 Sep 6. PMID:22975312<ref>PMID:22975312</ref> | |
| - | + | ||
| - | == | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> |
| - | + | </div> | |
| + | == References == | ||
| + | <references/> | ||
| + | __TOC__ | ||
| + | </StructureSection> | ||
[[Category: Desulfovibrio vulgaris str. 'miyazaki f']] | [[Category: Desulfovibrio vulgaris str. 'miyazaki f']] | ||
| - | [[Category: Higuchi, Y | + | [[Category: Higuchi, Y]] |
| - | [[Category: Kihira, K | + | [[Category: Kihira, K]] |
| - | [[Category: Kitamura, M | + | [[Category: Kitamura, M]] |
| - | [[Category: Shibata, N | + | [[Category: Shibata, N]] |
| - | [[Category: Shomura, Y | + | [[Category: Shomura, Y]] |
[[Category: Gtpase]] | [[Category: Gtpase]] | ||
[[Category: Release factor]] | [[Category: Release factor]] | ||
[[Category: Translation]] | [[Category: Translation]] | ||
Revision as of 20:12, 25 December 2014
Crystal structure analysis of the translation factor RF3
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