3zet
From Proteopedia
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| - | + | ==Structure of a Salmonella typhimurium YgjD-YeaZ heterodimer== | |
| - | === | + | <StructureSection load='3zet' size='340' side='right' caption='[[3zet]], [[Resolution|resolution]] 2.31Å' scene=''> |
| - | + | == Structural highlights == | |
| - | + | <table><tr><td colspan='2'>[[3zet]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Salmonella_enterica_subsp._enterica_serovar_typhimurium_str._st4/74 Salmonella enterica subsp. enterica serovar typhimurium str. st4/74]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3ZET OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3ZET FirstGlance]. <br> | |
| - | ==Function== | + | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=AMP:ADENOSINE+MONOPHOSPHATE'>AMP</scene>, <scene name='pdbligand=CD:CADMIUM+ION'>CD</scene>, <scene name='pdbligand=TAM:TRIS(HYDROXYETHYL)AMINOMETHANE'>TAM</scene></td></tr> |
| + | <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[3zeu|3zeu]]</td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3zet FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3zet OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3zet RCSB], [http://www.ebi.ac.uk/pdbsum/3zet PDBsum]</span></td></tr> | ||
| + | </table> | ||
| + | == Function == | ||
[[http://www.uniprot.org/uniprot/E8XBD7_SALT4 E8XBD7_SALT4]] Required for the formation of a threonylcarbamoyl group on adenosine at position 37 (t(6)A37) in tRNAs that read codons beginning with adenine (By similarity).[HAMAP-Rule:MF_01445] | [[http://www.uniprot.org/uniprot/E8XBD7_SALT4 E8XBD7_SALT4]] Required for the formation of a threonylcarbamoyl group on adenosine at position 37 (t(6)A37) in tRNAs that read codons beginning with adenine (By similarity).[HAMAP-Rule:MF_01445] | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | YgjD from COG0533 is amongst a small group of highly conserved proteins present in all three domains of life. Various roles and biochemical functions (including sialoprotease and endonuclease activities) have been ascribed to YgjD and orthologs, the most recent, however, is involvement in the post transcriptional modification of certain tRNAs by formation of N6-threonyl-adenosine (t6 A) at position 37. In bacteria, YgjD is essential and along with YeaZ, YjeE and YrdC has been shown to be 'necessary and sufficient' for the tRNA modification. To further define interactions and possible roles for some of this set of proteins we have undertaken structural and biochemical studies. We show that formation of the previously reported heterodimer of YgjD-YeaZ involves ordering of the C-terminal region of YeaZ which extends along the surface of YgjD in the crystal structure. ATPgammaS or AMP are observed in YgjD whilst no nucleotide is bound on YeaZ. | ||
| + | |||
| + | Crystal structure of the dimer of two essential Salmonella typhimurium proteins, YgjD & YeaZ and calorimetric evidence for the formation of a ternary YgjD-YeaZ-YjeE complex.,Nichols CE, Lamb HK, Thompson P, Omari KE, Lockyer M, Charles I, Hawkins AR, Stammers DK Protein Sci. 2013 Mar 8. doi: 10.1002/pro.2247. PMID:23471679<ref>PMID:23471679</ref> | ||
| - | == | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> |
| - | + | </div> | |
| + | == References == | ||
| + | <references/> | ||
| + | __TOC__ | ||
| + | </StructureSection> | ||
[[Category: Salmonella enterica subsp. enterica serovar typhimurium str. st4/74]] | [[Category: Salmonella enterica subsp. enterica serovar typhimurium str. st4/74]] | ||
| - | [[Category: Charles, I | + | [[Category: Charles, I]] |
| - | [[Category: Hawkins, A R | + | [[Category: Hawkins, A R]] |
| - | [[Category: Lamb, H K | + | [[Category: Lamb, H K]] |
| - | [[Category: Lockyer, M | + | [[Category: Lockyer, M]] |
| - | [[Category: Nichols, C E | + | [[Category: Nichols, C E]] |
| - | [[Category: Omari, K El | + | [[Category: Omari, K El]] |
| - | [[Category: Stammers, D K | + | [[Category: Stammers, D K]] |
| - | [[Category: Thompson, P | + | [[Category: Thompson, P]] |
[[Category: Hydrolase]] | [[Category: Hydrolase]] | ||
[[Category: Nucleotide binding]] | [[Category: Nucleotide binding]] | ||
[[Category: Yjee]] | [[Category: Yjee]] | ||
Revision as of 20:20, 25 December 2014
Structure of a Salmonella typhimurium YgjD-YeaZ heterodimer
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