2fct

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[[Image:2fct.gif|left|200px]]<br /><applet load="2fct" size="350" color="white" frame="true" align="right" spinBox="true"
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[[Image:2fct.gif|left|200px]]
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caption="2fct, resolution 1.60&Aring;" />
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'''SyrB2 with Fe(II), chloride, and alpha-ketoglutarate'''<br />
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{{Structure
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|PDB= 2fct |SIZE=350|CAPTION= <scene name='initialview01'>2fct</scene>, resolution 1.60&Aring;
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|SITE=
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|LIGAND= <scene name='pdbligand=FE2:FE+(II)+ION'>FE2</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=DSU:((2R,3S,4S,5S)-3,4-DIHYDROXY-5-(HYDROXYMETHYL)-5-((2R,3S,4S,5S,6R)-3,4,5-TRIHYDROXY-6-METHOXY-TETRAHYDRO-2H-PYRAN-2-YLOXY)-TETRAHYDROFURAN-2-YL)METHYL+NONANOATE'>DSU</scene> and <scene name='pdbligand=AKG:2-OXYGLUTARIC ACID'>AKG</scene>
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|ACTIVITY=
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|GENE= SyrB2 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=321 Pseudomonas syringae pv. syringae])
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}}
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'''SyrB2 with Fe(II), chloride, and alpha-ketoglutarate'''
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==Overview==
==Overview==
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==About this Structure==
==About this Structure==
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2FCT is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Pseudomonas_syringae_pv._syringae Pseudomonas syringae pv. syringae] with <scene name='pdbligand=FE2:'>FE2</scene>, <scene name='pdbligand=CL:'>CL</scene>, <scene name='pdbligand=DSU:'>DSU</scene> and <scene name='pdbligand=AKG:'>AKG</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2FCT OCA].
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2FCT is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Pseudomonas_syringae_pv._syringae Pseudomonas syringae pv. syringae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2FCT OCA].
==Reference==
==Reference==
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Crystal structure of the non-haem iron halogenase SyrB2 in syringomycin biosynthesis., Blasiak LC, Vaillancourt FH, Walsh CT, Drennan CL, Nature. 2006 Mar 16;440(7082):368-71. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=16541079 16541079]
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Crystal structure of the non-haem iron halogenase SyrB2 in syringomycin biosynthesis., Blasiak LC, Vaillancourt FH, Walsh CT, Drennan CL, Nature. 2006 Mar 16;440(7082):368-71. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/16541079 16541079]
[[Category: Pseudomonas syringae pv. syringae]]
[[Category: Pseudomonas syringae pv. syringae]]
[[Category: Single protein]]
[[Category: Single protein]]
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[[Category: mononuclear iron]]
[[Category: mononuclear iron]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 17:20:03 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 16:50:50 2008''

Revision as of 14:50, 20 March 2008

Image:2fct.gif


PDB ID 2fct

Drag the structure with the mouse to rotate
, resolution 1.60Å
Ligands: , , and
Gene: SyrB2 (Pseudomonas syringae pv. syringae)
Coordinates: save as pdb, mmCIF, xml



SyrB2 with Fe(II), chloride, and alpha-ketoglutarate


Overview

Non-haem Fe(II)/alpha-ketoglutarate (alphaKG)-dependent enzymes harness the reducing power of alphaKG to catalyse oxidative reactions, usually the hydroxylation of unactivated carbons, and are involved in processes such as natural product biosynthesis, the mammalian hypoxic response, and DNA repair. These enzymes couple the decarboxylation of alphaKG with the formation of a high-energy ferryl-oxo intermediate that acts as a hydrogen-abstracting species. All previously structurally characterized mononuclear iron enzymes contain a 2-His, 1-carboxylate motif that coordinates the iron. The two histidines and one carboxylate, known as the 'facial triad', form one triangular side of an octahedral iron coordination geometry. A subclass of mononuclear iron enzymes has been shown to catalyse halogenation reactions, rather than the more typical hydroxylation reaction. SyrB2, a member of this subclass, is a non-haem Fe(II)/alphaKG-dependent halogenase that catalyses the chlorination of threonine in syringomycin E biosynthesis. Here we report the structure of SyrB2 with both a chloride ion and alphaKG coordinated to the iron ion at 1.6 A resolution. This structure reveals a previously unknown coordination of iron, in which the carboxylate ligand of the facial triad is replaced by a chloride ion.

About this Structure

2FCT is a Single protein structure of sequence from Pseudomonas syringae pv. syringae. Full crystallographic information is available from OCA.

Reference

Crystal structure of the non-haem iron halogenase SyrB2 in syringomycin biosynthesis., Blasiak LC, Vaillancourt FH, Walsh CT, Drennan CL, Nature. 2006 Mar 16;440(7082):368-71. PMID:16541079

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