2fe9

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[[Image:2fe9.gif|left|200px]]<br /><applet load="2fe9" size="350" color="white" frame="true" align="right" spinBox="true"
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[[Image:2fe9.gif|left|200px]]
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caption="2fe9" />
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'''Solution structure of the Vts1 SAM domain in the presence of RNA'''<br />
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{{Structure
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|PDB= 2fe9 |SIZE=350|CAPTION= <scene name='initialview01'>2fe9</scene>
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|SITE=
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|LIGAND=
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|ACTIVITY=
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|GENE= VTS1 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=4932 Saccharomyces cerevisiae])
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}}
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'''Solution structure of the Vts1 SAM domain in the presence of RNA'''
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==Overview==
==Overview==
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==About this Structure==
==About this Structure==
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2FE9 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2FE9 OCA].
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2FE9 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2FE9 OCA].
==Reference==
==Reference==
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Solution structure of the Vts1 SAM domain in the presence of RNA., Edwards TA, Butterwick JA, Zeng L, Gupta YK, Wang X, Wharton RP, Palmer AG 3rd, Aggarwal AK, J Mol Biol. 2006 Mar 10;356(5):1065-72. Epub 2005 Dec 20. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=16405996 16405996]
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Solution structure of the Vts1 SAM domain in the presence of RNA., Edwards TA, Butterwick JA, Zeng L, Gupta YK, Wang X, Wharton RP, Palmer AG 3rd, Aggarwal AK, J Mol Biol. 2006 Mar 10;356(5):1065-72. Epub 2005 Dec 20. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/16405996 16405996]
[[Category: Saccharomyces cerevisiae]]
[[Category: Saccharomyces cerevisiae]]
[[Category: Single protein]]
[[Category: Single protein]]
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[[Category: vts1]]
[[Category: vts1]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 17:20:29 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 16:51:23 2008''

Revision as of 14:51, 20 March 2008


PDB ID 2fe9

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Gene: VTS1 (Saccharomyces cerevisiae)
Coordinates: save as pdb, mmCIF, xml



Solution structure of the Vts1 SAM domain in the presence of RNA


Overview

The yeast Vts1 SAM (sterile alpha motif) domain is a member of a new class of SAM domains that specifically bind RNA. To elucidate the structural basis for RNA binding, the solution structure of the Vts1 SAM domain, in the presence of a specific target RNA, has been solved by multidimensional heteronuclear NMR spectroscopy. The Vts1 SAM domain retains the "core" five-helix-bundle architecture of traditional SAM domains, but has additional short helices at N and C termini, comprising a small substructure that caps the core helices. The RNA-binding surface of Vts1, determined by chemical shift perturbation, maps near the ends of three of the core helices, in agreement with mutational data and the electrostatic properties of the molecule. These results provide a structural basis for the versatility of the SAM domain in protein and RNA-recognition.

About this Structure

2FE9 is a Single protein structure of sequence from Saccharomyces cerevisiae. Full crystallographic information is available from OCA.

Reference

Solution structure of the Vts1 SAM domain in the presence of RNA., Edwards TA, Butterwick JA, Zeng L, Gupta YK, Wang X, Wharton RP, Palmer AG 3rd, Aggarwal AK, J Mol Biol. 2006 Mar 10;356(5):1065-72. Epub 2005 Dec 20. PMID:16405996

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