2fij

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[[Image:2fij.gif|left|200px]]<br /><applet load="2fij" size="350" color="white" frame="true" align="right" spinBox="true"
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[[Image:2fij.gif|left|200px]]
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caption="2fij, resolution 1.19&Aring;" />
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'''Crystal Structure Analysis of the A-DNA Decamer GCGT-2'OMeA-aU-ACGC, with Incorporated 2'-O-Methylated-Adenosine (2'OMeA) and Arabino-Uridine (aU)'''<br />
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{{Structure
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|PDB= 2fij |SIZE=350|CAPTION= <scene name='initialview01'>2fij</scene>, resolution 1.19&Aring;
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|SITE=
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|LIGAND= <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene> and <scene name='pdbligand=NCO:COBALT HEXAMMINE ION'>NCO</scene>
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|ACTIVITY=
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|GENE=
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}}
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'''Crystal Structure Analysis of the A-DNA Decamer GCGT-2'OMeA-aU-ACGC, with Incorporated 2'-O-Methylated-Adenosine (2'OMeA) and Arabino-Uridine (aU)'''
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==Overview==
==Overview==
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==About this Structure==
==About this Structure==
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2FIJ is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/ ] with <scene name='pdbligand=CL:'>CL</scene> and <scene name='pdbligand=NCO:'>NCO</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2FIJ OCA].
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2FIJ is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/ ]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2FIJ OCA].
==Reference==
==Reference==
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2'-Fluoroarabino- and arabinonucleic acid show different conformations, resulting in deviating RNA affinities and processing of their heteroduplexes with RNA by RNase H., Li F, Sarkhel S, Wilds CJ, Wawrzak Z, Prakash TP, Manoharan M, Egli M, Biochemistry. 2006 Apr 4;45(13):4141-52. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=16566588 16566588]
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2'-Fluoroarabino- and arabinonucleic acid show different conformations, resulting in deviating RNA affinities and processing of their heteroduplexes with RNA by RNase H., Li F, Sarkhel S, Wilds CJ, Wawrzak Z, Prakash TP, Manoharan M, Egli M, Biochemistry. 2006 Apr 4;45(13):4141-52. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/16566588 16566588]
[[Category: Protein complex]]
[[Category: Protein complex]]
[[Category: Egli, M.]]
[[Category: Egli, M.]]
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[[Category: a-form dna]]
[[Category: a-form dna]]
[[Category: arabinonucleic acid]]
[[Category: arabinonucleic acid]]
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[[Category: sugar modifications]]
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[[Category: sugar modification]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 17:21:43 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 16:52:49 2008''

Revision as of 14:52, 20 March 2008

Image:2fij.gif


PDB ID 2fij

Drag the structure with the mouse to rotate
, resolution 1.19Å
Ligands: and
Coordinates: save as pdb, mmCIF, xml



Crystal Structure Analysis of the A-DNA Decamer GCGT-2'OMeA-aU-ACGC, with Incorporated 2'-O-Methylated-Adenosine (2'OMeA) and Arabino-Uridine (aU)


Overview

2'-Deoxy-2'-fluoro-arabinonucleic acid (FANA) and arabinonucleic acid (ANA) paired to RNA are substrates of RNase H. The conformation of the natural DNA/RNA hybrid substrates appears to be neither A-form nor B-form. Consistent with this, the conformations of FANA and ANA were found to be intermediate between the A- and B-forms. However, FANA opposite RNA is preferred by RNase H over ANA, and the RNA affinity of FANA considerably exceeds that of ANA. By investigating the conformational boundaries of FANA and ANA residues in crystal structures of A- and B-form DNA duplexes at atomic resolution, we demonstrate that FANA and ANA display subtle conformational differences. The structural data provide insight into the structural requirements at the catalytic site of RNase H. They also allow conclusions with regard to the relative importance of stereoelectronic effects and hydration as modulators of RNA affinity.

About this Structure

2FIJ is a Protein complex structure of sequences from [1]. Full crystallographic information is available from OCA.

Reference

2'-Fluoroarabino- and arabinonucleic acid show different conformations, resulting in deviating RNA affinities and processing of their heteroduplexes with RNA by RNase H., Li F, Sarkhel S, Wilds CJ, Wawrzak Z, Prakash TP, Manoharan M, Egli M, Biochemistry. 2006 Apr 4;45(13):4141-52. PMID:16566588

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