2fj7
From Proteopedia
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| - | [[Image:2fj7.gif|left|200px]] | + | [[Image:2fj7.gif|left|200px]] |
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| - | '''Crystal structure of Nucleosome Core Particle Containing a Poly (dA.dT) Sequence Element''' | + | {{Structure |
| + | |PDB= 2fj7 |SIZE=350|CAPTION= <scene name='initialview01'>2fj7</scene>, resolution 3.2Å | ||
| + | |SITE= | ||
| + | |LIGAND= | ||
| + | |ACTIVITY= | ||
| + | |GENE= | ||
| + | }} | ||
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| + | '''Crystal structure of Nucleosome Core Particle Containing a Poly (dA.dT) Sequence Element''' | ||
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==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
| - | 2FJ7 is a [ | + | 2FJ7 is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/Xenopus_laevis Xenopus laevis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2FJ7 OCA]. |
==Reference== | ==Reference== | ||
| - | Nucleosome core particles containing a poly(dA.dT) sequence element exhibit a locally distorted DNA structure., Bao Y, White CL, Luger K, J Mol Biol. 2006 Aug 25;361(4):617-24. Epub 2006 Jul 5. PMID:[http:// | + | Nucleosome core particles containing a poly(dA.dT) sequence element exhibit a locally distorted DNA structure., Bao Y, White CL, Luger K, J Mol Biol. 2006 Aug 25;361(4):617-24. Epub 2006 Jul 5. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/16860337 16860337] |
[[Category: Protein complex]] | [[Category: Protein complex]] | ||
[[Category: Xenopus laevis]] | [[Category: Xenopus laevis]] | ||
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[[Category: protein-dna complex]] | [[Category: protein-dna complex]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 16:53:03 2008'' |
Revision as of 14:53, 20 March 2008
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| Coordinates: | save as pdb, mmCIF, xml | ||||||
Crystal structure of Nucleosome Core Particle Containing a Poly (dA.dT) Sequence Element
Overview
Poly(dA.dT) DNA sequence elements are thought to promote transcription by either excluding nucleosomes or by altering their structural or dynamic properties. Here, the stability and structure of a defined nucleosome core particle containing a 16 base-pair poly(dA.dT) element (A16 NCP) was investigated. The A16 NCP requires a significantly higher temperature for histone octamer sliding in vitro compared to comparable nucleosomes that do not contain a poly(dA.dT) element. Fluorescence resonance energy transfer showed that the interactions between the nucleosomal DNA ends and the histone octamer were destabilized in A16 NCP. The crystal structure of A16 NCP was determined to a resolution of 3.2 A. The overall structure was maintained except for local deviations in DNA conformation. These results are consistent with previous in vivo and in vitro observations that poly(dA.dT) elements cause only modest changes in DNA accessibility and modest increases in steady-state transcription levels.
About this Structure
2FJ7 is a Protein complex structure of sequences from Xenopus laevis. Full crystallographic information is available from OCA.
Reference
Nucleosome core particles containing a poly(dA.dT) sequence element exhibit a locally distorted DNA structure., Bao Y, White CL, Luger K, J Mol Biol. 2006 Aug 25;361(4):617-24. Epub 2006 Jul 5. PMID:16860337
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