2flh
From Proteopedia
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| - | [[Image:2flh.gif|left|200px]] | + | [[Image:2flh.gif|left|200px]] |
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| - | '''Crystal structure of cytokinin-specific binding protein from mung bean in complex with cytokinin''' | + | {{Structure |
| + | |PDB= 2flh |SIZE=350|CAPTION= <scene name='initialview01'>2flh</scene>, resolution 1.20Å | ||
| + | |SITE= | ||
| + | |LIGAND= <scene name='pdbligand=NA:SODIUM+ION'>NA</scene> and <scene name='pdbligand=ZEA:(2Z)-2-METHYL-4-(9H-PURIN-6-YLAMINO)-2-BUTEN-1-OL'>ZEA</scene> | ||
| + | |ACTIVITY= | ||
| + | |GENE= vrcsbp ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=157791 Vigna radiata]) | ||
| + | }} | ||
| + | |||
| + | '''Crystal structure of cytokinin-specific binding protein from mung bean in complex with cytokinin''' | ||
| + | |||
==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
| - | 2FLH is a [ | + | 2FLH is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Vigna_radiata Vigna radiata]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2FLH OCA]. |
==Reference== | ==Reference== | ||
| - | Crystal structure of Vigna radiata cytokinin-specific binding protein in complex with zeatin., Pasternak O, Bujacz GD, Fujimoto Y, Hashimoto Y, Jelen F, Otlewski J, Sikorski MM, Jaskolski M, Plant Cell. 2006 Oct;18(10):2622-34. Epub 2006 Sep 22. PMID:[http:// | + | Crystal structure of Vigna radiata cytokinin-specific binding protein in complex with zeatin., Pasternak O, Bujacz GD, Fujimoto Y, Hashimoto Y, Jelen F, Otlewski J, Sikorski MM, Jaskolski M, Plant Cell. 2006 Oct;18(10):2622-34. Epub 2006 Sep 22. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/16998071 16998071] |
[[Category: Single protein]] | [[Category: Single protein]] | ||
[[Category: Vigna radiata]] | [[Category: Vigna radiata]] | ||
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[[Category: NA]] | [[Category: NA]] | ||
[[Category: ZEA]] | [[Category: ZEA]] | ||
| - | [[Category: | + | [[Category: cytokinin]] |
[[Category: multiple-ligand binding]] | [[Category: multiple-ligand binding]] | ||
| - | [[Category: pathogenesis-related | + | [[Category: pathogenesis-related protein]] |
[[Category: zeatin]] | [[Category: zeatin]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 16:53:49 2008'' |
Revision as of 14:53, 20 March 2008
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| , resolution 1.20Å | |||||||
|---|---|---|---|---|---|---|---|
| Ligands: | and | ||||||
| Gene: | vrcsbp (Vigna radiata) | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
Crystal structure of cytokinin-specific binding protein from mung bean in complex with cytokinin
Overview
The cytosolic fraction of Vigna radiata contains a 17-kD protein that binds plant hormones from the cytokinin group, such as zeatin. Using recombinant protein and isothermal titration calorimetry as well as fluorescence measurements coupled with ligand displacement, we have reexamined the K(d) values and show them to range from approximately 10(-6) M (for 4PU30) to 10(-4) M (for zeatin) for 1:1 stoichiometry complexes. In addition, we have crystallized this cytokinin-specific binding protein (Vr CSBP) in complex with zeatin and refined the structure to 1.2 A resolution. Structurally, Vr CSBP is similar to plant pathogenesis-related class 10 (PR-10) proteins, despite low sequence identity (<20%). This unusual fold conservation reinforces the notion that classic PR-10 proteins have evolved to bind small-molecule ligands. The fold consists of an antiparallel beta-sheet wrapped around a C-terminal alpha-helix, with two short alpha-helices closing a cavity formed within the protein core. In each of the four independent CSBP molecules, there is a zeatin ligand located deep in the cavity with conserved conformation and protein-ligand interactions. In three cases, an additional zeatin molecule is found in variable orientation but with excellent definition in electron density, which plugs the entrance to the binding pocket, sealing the inner molecule from contact with bulk solvent.
About this Structure
2FLH is a Single protein structure of sequence from Vigna radiata. Full crystallographic information is available from OCA.
Reference
Crystal structure of Vigna radiata cytokinin-specific binding protein in complex with zeatin., Pasternak O, Bujacz GD, Fujimoto Y, Hashimoto Y, Jelen F, Otlewski J, Sikorski MM, Jaskolski M, Plant Cell. 2006 Oct;18(10):2622-34. Epub 2006 Sep 22. PMID:16998071
Page seeded by OCA on Thu Mar 20 16:53:49 2008
