4cs5
From Proteopedia
(Difference between revisions)
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| - | + | ==Crystal Structure of PCNA from Litopenaeus vannamei== | |
| - | + | <StructureSection load='4cs5' size='340' side='right' caption='[[4cs5]], [[Resolution|resolution]] 3.00Å' scene=''> | |
| - | + | == Structural highlights == | |
| - | + | <table><tr><td colspan='2'>[[4cs5]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/Litva Litva]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4CS5 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4CS5 FirstGlance]. <br> | |
| - | ==Function== | + | </td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4cs5 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4cs5 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4cs5 RCSB], [http://www.ebi.ac.uk/pdbsum/4cs5 PDBsum]</span></td></tr> |
| + | </table> | ||
| + | == Function == | ||
[[http://www.uniprot.org/uniprot/G1E6N7_LITVA G1E6N7_LITVA]] This protein is an auxiliary protein of DNA polymerase delta and is involved in the control of eukaryotic DNA replication by increasing the polymerase's processibility during elongation of the leading strand (By similarity).[RuleBase:RU000641] | [[http://www.uniprot.org/uniprot/G1E6N7_LITVA G1E6N7_LITVA]] This protein is an auxiliary protein of DNA polymerase delta and is involved in the control of eukaryotic DNA replication by increasing the polymerase's processibility during elongation of the leading strand (By similarity).[RuleBase:RU000641] | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | DNA replication requires processivity factors that allow replicative DNA polymerases to extend long stretches of DNA. Some DNA viruses encode their own replicative DNA polymerase, such as the white spot syndrome virus (WSSV) that infects decapod crustaceans but still require host replication accessory factors. We have determined by X-ray diffraction the three-dimensional structure of the Pacific white leg shrimp Litopenaeus vannamei Proliferating Cell Nuclear Antigen (LvPCNA). This protein is a member of the sliding clamp family of proteins, that binds DNA replication and DNA repair proteins through a motif called PIP-box (PCNA-Interacting Protein). The crystal structure of LvPCNA was refined to a resolution of 3 A, and allowed us to determine the trimeric protein assembly and details of the interactions between PCNA and the DNA. To address the possible interaction between LvPCNA and the viral DNA polymerase, we docked a theoretical model of a PIP-box peptide from the WSSV DNA polymerase within LvPCNA crystal structure. The theoretical model depicts a feasible model of interaction between both proteins. The crystal structure of shrimp PCNA allows us to further understand the mechanisms of DNA replication processivity factors in non-model systems. | ||
| - | + | Crystal Structure of the Shrimp Proliferating Cell Nuclear Antigen: Structural Complementarity with WSSV DNA Polymerase PIP-Box.,Carrasco-Miranda JS, Lopez-Zavala AA, Arvizu-Flores AA, Garcia-Orozco KD, Stojanoff V, Rudino-Pinera E, Brieba LG, Sotelo-Mundo RR PLoS One. 2014 Apr 11;9(4):e94369. doi: 10.1371/journal.pone.0094369. eCollection, 2014. PMID:24728082<ref>PMID:24728082</ref> | |
| - | + | ||
| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| - | < | + | </div> |
| - | [[Category: Brieba, L G | + | == References == |
| - | [[Category: Carrasco-Miranda, J S | + | <references/> |
| - | [[Category: De-La-Mora, E | + | __TOC__ |
| - | [[Category: Lopez-Zavala, A A | + | </StructureSection> |
| - | [[Category: Rudino-Pinera, E | + | [[Category: Litva]] |
| - | [[Category: Sotelo-Mundo, R R | + | [[Category: Brieba, L G]] |
| + | [[Category: Carrasco-Miranda, J S]] | ||
| + | [[Category: De-La-Mora, E]] | ||
| + | [[Category: Lopez-Zavala, A A]] | ||
| + | [[Category: Rudino-Pinera, E]] | ||
| + | [[Category: Sotelo-Mundo, R R]] | ||
[[Category: Dna binding protein]] | [[Category: Dna binding protein]] | ||
[[Category: Nuclear protein]] | [[Category: Nuclear protein]] | ||
Revision as of 22:23, 25 December 2014
Crystal Structure of PCNA from Litopenaeus vannamei
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