2fmx
From Proteopedia
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| - | [[Image:2fmx.jpg|left|200px]] | + | [[Image:2fmx.jpg|left|200px]] |
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| - | '''An open conformation of switch I revealed by Sar1-GDP crystal structure at low Mg(2+)''' | + | {{Structure |
| + | |PDB= 2fmx |SIZE=350|CAPTION= <scene name='initialview01'>2fmx</scene>, resolution 1.82Å | ||
| + | |SITE= | ||
| + | |LIGAND= <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene> and <scene name='pdbligand=GDP:GUANOSINE-5'-DIPHOSPHATE'>GDP</scene> | ||
| + | |ACTIVITY= | ||
| + | |GENE= | ||
| + | }} | ||
| + | |||
| + | '''An open conformation of switch I revealed by Sar1-GDP crystal structure at low Mg(2+)''' | ||
| + | |||
==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
| - | 2FMX is a [ | + | 2FMX is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Cricetulus_griseus Cricetulus griseus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2FMX OCA]. |
==Reference== | ==Reference== | ||
| - | An open conformation of switch I revealed by Sar1-GDP crystal structure at low Mg2+., Rao Y, Bian C, Yuan C, Li Y, Chen L, Ye X, Huang Z, Huang M, Biochem Biophys Res Commun. 2006 Sep 29;348(3):908-15. Epub 2006 Aug 1. PMID:[http:// | + | An open conformation of switch I revealed by Sar1-GDP crystal structure at low Mg2+., Rao Y, Bian C, Yuan C, Li Y, Chen L, Ye X, Huang Z, Huang M, Biochem Biophys Res Commun. 2006 Sep 29;348(3):908-15. Epub 2006 Aug 1. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/16899220 16899220] |
[[Category: Cricetulus griseus]] | [[Category: Cricetulus griseus]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
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[[Category: sar1]] | [[Category: sar1]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 16:54:22 2008'' |
Revision as of 14:54, 20 March 2008
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| , resolution 1.82Å | |||||||
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| Ligands: | , and | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
An open conformation of switch I revealed by Sar1-GDP crystal structure at low Mg(2+)
Overview
Mg2+ is essential for guanosine triphosphatase activity and plays key roles in guanine nucleotide binding and preserving the structural integrity of GTP-binding proteins. To understand the structural basis for Mg2+ function during the GDP/GTP exchange process, we determined the crystal structure of Delta9-Sar1-GDP at low Mg2+ concentration at 1.8A. Two Sar1-GDP molecules in the crystal form a dimer with Mg2+ presenting only in molecule B but not in molecule A. The absence of Mg2+ induces significant conformational changes in the switch I region in molecule A that shows similarities with those of Ha-Ras bound to Sos. The current structure reveals an important regulatory role for Mg2+. We suggest that guanine nucleotide exchange factor may utilize this feature to generate an open conformation for GDP/GTP exchange. Furthermore, we propose a mechanism for COPII assembly and disassembly in which dimerization of Sar1 plays an important role.
About this Structure
2FMX is a Single protein structure of sequence from Cricetulus griseus. Full crystallographic information is available from OCA.
Reference
An open conformation of switch I revealed by Sar1-GDP crystal structure at low Mg2+., Rao Y, Bian C, Yuan C, Li Y, Chen L, Ye X, Huang Z, Huang M, Biochem Biophys Res Commun. 2006 Sep 29;348(3):908-15. Epub 2006 Aug 1. PMID:16899220
Page seeded by OCA on Thu Mar 20 16:54:22 2008
Categories: Cricetulus griseus | Single protein | Bian, C. | Huang, M. | Li, Y. | Rao, Y. | Yuan, C. | GDP | MG | SO4 | Cop ii assembly | Crystal structure | Dimerization | Sar1
