2fts
From Proteopedia
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| - | [[Image:2fts.gif|left|200px]] | + | [[Image:2fts.gif|left|200px]] |
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| - | '''Crystal structure of the glycine receptor-gephyrin complex''' | + | {{Structure |
| + | |PDB= 2fts |SIZE=350|CAPTION= <scene name='initialview01'>2fts</scene>, resolution 2.410Å | ||
| + | |SITE= | ||
| + | |LIGAND= | ||
| + | |ACTIVITY= | ||
| + | |GENE= Rattus norvegicus (Norway rat) ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10116 Rattus norvegicus]), Glrb ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10116 Rattus norvegicus]) | ||
| + | }} | ||
| + | |||
| + | '''Crystal structure of the glycine receptor-gephyrin complex''' | ||
| + | |||
==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
| - | 2FTS is a [ | + | 2FTS is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/Rattus_norvegicus Rattus norvegicus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2FTS OCA]. |
==Reference== | ==Reference== | ||
| - | Deciphering the structural framework of glycine receptor anchoring by gephyrin., Kim EY, Schrader N, Smolinsky B, Bedet C, Vannier C, Schwarz G, Schindelin H, EMBO J. 2006 Mar 22;25(6):1385-95. Epub 2006 Mar 2. PMID:[http:// | + | Deciphering the structural framework of glycine receptor anchoring by gephyrin., Kim EY, Schrader N, Smolinsky B, Bedet C, Vannier C, Schwarz G, Schindelin H, EMBO J. 2006 Mar 22;25(6):1385-95. Epub 2006 Mar 2. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/16511563 16511563] |
[[Category: Protein complex]] | [[Category: Protein complex]] | ||
[[Category: Rattus norvegicus]] | [[Category: Rattus norvegicus]] | ||
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[[Category: neuroreceptor anchoring]] | [[Category: neuroreceptor anchoring]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 16:56:40 2008'' |
Revision as of 14:56, 20 March 2008
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| , resolution 2.410Å | |||||||
|---|---|---|---|---|---|---|---|
| Gene: | Rattus norvegicus (Norway rat) (Rattus norvegicus), Glrb (Rattus norvegicus) | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
Crystal structure of the glycine receptor-gephyrin complex
Overview
Glycine is the major inhibitory neurotransmitter in the spinal cord and brain stem. Gephyrin is required to achieve a high concentration of glycine receptors (GlyRs) in the postsynaptic membrane, which is crucial for efficient glycinergic signal transduction. The interaction between gephyrin and the GlyR involves the E-domain of gephyrin and a cytoplasmic loop located between transmembrane segments three and four of the GlyR beta subunit. Here, we present crystal structures of the gephyrin E-domain with and without the GlyR beta-loop at 2.4 and 2.7 A resolutions, respectively. The GlyR beta-loop is bound in a symmetric 'key and lock' fashion to each E-domain monomer in a pocket adjacent to the dimer interface. Structure-guided mutagenesis followed by in vitro binding and in vivo colocalization assays demonstrate that a hydrophobic interaction formed by Phe 330 of gephyrin and Phe 398 and Ile 400 of the GlyR beta-loop is crucial for binding.
About this Structure
2FTS is a Protein complex structure of sequences from Rattus norvegicus. Full crystallographic information is available from OCA.
Reference
Deciphering the structural framework of glycine receptor anchoring by gephyrin., Kim EY, Schrader N, Smolinsky B, Bedet C, Vannier C, Schwarz G, Schindelin H, EMBO J. 2006 Mar 22;25(6):1385-95. Epub 2006 Mar 2. PMID:16511563
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