2fub

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[[Image:2fub.gif|left|200px]]<br /><applet load="2fub" size="350" color="white" frame="true" align="right" spinBox="true"
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[[Image:2fub.gif|left|200px]]
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caption="2fub, resolution 2.30&Aring;" />
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'''Crystal structure of urate oxidase at 140 MPa'''<br />
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{{Structure
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|PDB= 2fub |SIZE=350|CAPTION= <scene name='initialview01'>2fub</scene>, resolution 2.30&Aring;
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|SITE=
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|LIGAND= <scene name='pdbligand=AZA:8-AZAXANTHINE'>AZA</scene> and <scene name='pdbligand=CYS:CYSTEINE'>CYS</scene>
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|ACTIVITY= [http://en.wikipedia.org/wiki/Urate_oxidase Urate oxidase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.7.3.3 1.7.3.3]
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|GENE=
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}}
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'''Crystal structure of urate oxidase at 140 MPa'''
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==Overview==
==Overview==
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==About this Structure==
==About this Structure==
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2FUB is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Aspergillus_flavus Aspergillus flavus] with <scene name='pdbligand=AZA:'>AZA</scene> and <scene name='pdbligand=CYS:'>CYS</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Urate_oxidase Urate oxidase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.7.3.3 1.7.3.3] Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2FUB OCA].
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2FUB is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Aspergillus_flavus Aspergillus flavus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2FUB OCA].
==Reference==
==Reference==
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High pressure macromolecular crystallography: the 140-MPa crystal structure at 2.3 A resolution of urate oxidase, a 135-kDa tetrameric assembly., Colloc'h N, Girard E, Dhaussy AC, Kahn R, Ascone I, Mezouar M, Fourme R, Biochim Biophys Acta. 2006 Mar;1764(3):391-7. Epub 2006 Jan 26. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=16478683 16478683]
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High pressure macromolecular crystallography: the 140-MPa crystal structure at 2.3 A resolution of urate oxidase, a 135-kDa tetrameric assembly., Colloc'h N, Girard E, Dhaussy AC, Kahn R, Ascone I, Mezouar M, Fourme R, Biochim Biophys Acta. 2006 Mar;1764(3):391-7. Epub 2006 Jan 26. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/16478683 16478683]
[[Category: Aspergillus flavus]]
[[Category: Aspergillus flavus]]
[[Category: Single protein]]
[[Category: Single protein]]
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[[Category: tetramer]]
[[Category: tetramer]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 17:25:12 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 16:56:48 2008''

Revision as of 14:56, 20 March 2008

Image:2fub.gif


PDB ID 2fub

Drag the structure with the mouse to rotate
, resolution 2.30Å
Ligands: and
Activity: Urate oxidase, with EC number 1.7.3.3
Coordinates: save as pdb, mmCIF, xml



Crystal structure of urate oxidase at 140 MPa


Overview

We report the three-dimensional structure determined by high-pressure macromolecular crystallography (HPMX) of a 135-kDa homo-tetrameric enzyme, urate oxidase from Aspergillus flavus complexed with its potent inhibitor 8-azaxanthin. Urate oxidase crystals are quite sensitive to pressure, as three-dimensional order is lost at about 180 MPa. A highly complete 2.3 A resolution data set was collected at 140 MPa, close to the critical pressure. Crystal structures at atmospheric pressure and at high pressure were refined in the orthorhombic space group I222 with final crystallographic R factors 14.1% and 16.1%, respectively. The effect of pressure on temperature factors, ordered water molecules, hydrogen bond lengths, contacts, buried surface areas as well as cavity volume was investigated. Results suggest that the onset of disruption of the tetrameric assembly by pressure has been captured in the crystalline state.

About this Structure

2FUB is a Single protein structure of sequence from Aspergillus flavus. Full crystallographic information is available from OCA.

Reference

High pressure macromolecular crystallography: the 140-MPa crystal structure at 2.3 A resolution of urate oxidase, a 135-kDa tetrameric assembly., Colloc'h N, Girard E, Dhaussy AC, Kahn R, Ascone I, Mezouar M, Fourme R, Biochim Biophys Acta. 2006 Mar;1764(3):391-7. Epub 2006 Jan 26. PMID:16478683

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