4w94
From Proteopedia
(Difference between revisions)
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| - | ''' | + | ==Crystal structure of cross-linked tetragonal hen egg white lysozyme soaked with 5mM [Ru(CO)3Cl2]2== |
| + | <StructureSection load='4w94' size='340' side='right' caption='[[4w94]], [[Resolution|resolution]] 1.55Å' scene=''> | ||
| + | == Structural highlights == | ||
| + | <table><tr><td colspan='2'>[[4w94]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Gallus_gallus Gallus gallus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4W94 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4W94 FirstGlance]. <br> | ||
| + | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=DMF:DIMETHYLFORMAMIDE'>DMF</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene>, <scene name='pdbligand=RU:RUTHENIUM+ION'>RU</scene>, <scene name='pdbligand=RU1:BIS(OXIDANIUMYLIDYNEMETHYL)RUTHENIUM(2+)'>RU1</scene></td></tr> | ||
| + | <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[4w96|4w96]]</td></tr> | ||
| + | <tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Lysozyme Lysozyme], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.17 3.2.1.17] </span></td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4w94 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4w94 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4w94 RCSB], [http://www.ebi.ac.uk/pdbsum/4w94 PDBsum]</span></td></tr> | ||
| + | </table> | ||
| + | == Function == | ||
| + | [[http://www.uniprot.org/uniprot/LYSC_CHICK LYSC_CHICK]] Lysozymes have primarily a bacteriolytic function; those in tissues and body fluids are associated with the monocyte-macrophage system and enhance the activity of immunoagents. Has bacteriolytic activity against M.luteus.<ref>PMID:22044478</ref> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | Protein crystals generally are stable solid protein assemblies. Certain protein crystals are suitable for use as nanovessels for immobilizing metal complexes. Here we report the preparation of ruthenium carbonyl-incorporated cross-linked hen egg white lysozyme crystals (Ru.CL-HEWL). Ru.CL-HEWL retains a Ru carbonyl moiety that can release CO, although a composite of Ru carbonyl-HEWL dissolved in buffer solution (Ru.HEWL) does not release CO. We found that treatment of cells with Ru.CL-HEWL significantly increased nuclear factor kappa B (NF-kappaB) activity as a cellular response to CO. These results demonstrate that Ru.CL-HEWL has potential for use as an artificial extracellular scaffold suitable for transport and release of a gas molecule. | ||
| - | + | Preparation of a Cross-Linked Porous Protein Crystal Containing Ru Carbonyl Complexes as a CO-Releasing Extracellular Scaffold.,Tabe H, Fujita K, Abe S, Tsujimoto M, Kuchimaru T, Kizaka-Kondoh S, Takano M, Kitagawa S, Ueno T Inorg Chem. 2014 Dec 15. PMID:25494847<ref>PMID:25494847</ref> | |
| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| - | + | </div> | |
| - | + | == References == | |
| - | [[Category: | + | <references/> |
| + | __TOC__ | ||
| + | </StructureSection> | ||
| + | [[Category: Gallus gallus]] | ||
| + | [[Category: Lysozyme]] | ||
| + | [[Category: Abe, S]] | ||
[[Category: Fujita, K]] | [[Category: Fujita, K]] | ||
| + | [[Category: Kitagawa, S]] | ||
[[Category: Kizaka-Kondo, S]] | [[Category: Kizaka-Kondo, S]] | ||
| + | [[Category: Kuchimaru, T]] | ||
| + | [[Category: Tabe, H]] | ||
| + | [[Category: Takano, M]] | ||
[[Category: Tsujimoto, M]] | [[Category: Tsujimoto, M]] | ||
| - | [[Category: Kitagawa, S]] | ||
| - | [[Category: Takano, M]] | ||
| - | [[Category: Abe, S]] | ||
[[Category: Ueno, T]] | [[Category: Ueno, T]] | ||
| - | [[Category: | + | [[Category: Hydrolase]] |
| - | + | ||
Revision as of 14:46, 31 December 2014
Crystal structure of cross-linked tetragonal hen egg white lysozyme soaked with 5mM [Ru(CO)3Cl2]2
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Categories: Gallus gallus | Lysozyme | Abe, S | Fujita, K | Kitagawa, S | Kizaka-Kondo, S | Kuchimaru, T | Tabe, H | Takano, M | Tsujimoto, M | Ueno, T | Hydrolase
