4wbd
From Proteopedia
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| - | ''' | + | ==The crystal structure of BshC from Bacillus subtilis complexed with citrate and ADP== |
| + | <StructureSection load='4wbd' size='340' side='right' caption='[[4wbd]], [[Resolution|resolution]] 1.77Å' scene=''> | ||
| + | == Structural highlights == | ||
| + | <table><tr><td colspan='2'>[[4wbd]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4WBD OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4WBD FirstGlance]. <br> | ||
| + | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ADP:ADENOSINE-5-DIPHOSPHATE'>ADP</scene>, <scene name='pdbligand=CIT:CITRIC+ACID'>CIT</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene></td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4wbd FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4wbd OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4wbd RCSB], [http://www.ebi.ac.uk/pdbsum/4wbd PDBsum]</span></td></tr> | ||
| + | </table> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | Bacillithiol is produced by many Gram-positive bacteria via a pathway utilizing the enzymes BshA, BshB, and BshC. Here we report the 1.77 A resolution crystal structure of BshC, the putative cysteine ligase in bacillithiol production. The structure reveals that BshC contains a core Rossmann fold with connecting peptide motifs (CP1 and CP2) and a unique alpha-helical coiled-coil domain that facilitates dimerization. The model contains citrate and glycerol in the canonical active site and ADP in a second binding pocket. The overall structure and bound ligands give insight into the function of this unique enzyme. | ||
| - | + | X-ray Crystallographic Structure of BshC, a Unique Enzyme Involved in Bacillithiol Biosynthesis.,VanDuinen AJ, Winchell KR, Keithly ME, Cook PD Biochemistry. 2014 Dec 18. PMID:25496067<ref>PMID:25496067</ref> | |
| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| - | + | </div> | |
| - | + | == References == | |
| - | [[Category: | + | <references/> |
| - | [[Category: | + | __TOC__ |
| - | [[Category: Winchell, K | + | </StructureSection> |
| - | [[Category: | + | [[Category: Cook, P D]] |
| + | [[Category: VanDuinen, A J]] | ||
| + | [[Category: Winchell, K R]] | ||
| + | [[Category: Bacililthiol]] | ||
| + | [[Category: Cysteine]] | ||
| + | [[Category: Ligase]] | ||
| + | [[Category: Rossmann]] | ||
Revision as of 14:48, 31 December 2014
The crystal structure of BshC from Bacillus subtilis complexed with citrate and ADP
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Categories: Cook, P D | VanDuinen, A J | Winchell, K R | Bacililthiol | Cysteine | Ligase | Rossmann
