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2fx3

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[[Image:2fx3.gif|left|200px]]<br /><applet load="2fx3" size="350" color="white" frame="true" align="right" spinBox="true"
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[[Image:2fx3.gif|left|200px]]
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caption="2fx3, resolution 3.400&Aring;" />
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'''Crystal Structure Determination of E. coli Elongation Factor, Tu using a Twinned Data Set'''<br />
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{{Structure
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|PDB= 2fx3 |SIZE=350|CAPTION= <scene name='initialview01'>2fx3</scene>, resolution 3.400&Aring;
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|SITE=
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|LIGAND= <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene> and <scene name='pdbligand=GDP:GUANOSINE-5'-DIPHOSPHATE'>GDP</scene>
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|ACTIVITY=
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|GENE=
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}}
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'''Crystal Structure Determination of E. coli Elongation Factor, Tu using a Twinned Data Set'''
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==Overview==
==Overview==
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==About this Structure==
==About this Structure==
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2FX3 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli] with <scene name='pdbligand=MG:'>MG</scene> and <scene name='pdbligand=GDP:'>GDP</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2FX3 OCA].
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2FX3 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2FX3 OCA].
==Reference==
==Reference==
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Solving the structure of Escherichia coli elongation factor Tu using a twinned data set., Heffron SE, Moeller R, Jurnak F, Acta Crystallogr D Biol Crystallogr. 2006 Apr;62(Pt 4):433-8. Epub 2006, Mar 18. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=16552145 16552145]
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Solving the structure of Escherichia coli elongation factor Tu using a twinned data set., Heffron SE, Moeller R, Jurnak F, Acta Crystallogr D Biol Crystallogr. 2006 Apr;62(Pt 4):433-8. Epub 2006, Mar 18. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/16552145 16552145]
[[Category: Escherichia coli]]
[[Category: Escherichia coli]]
[[Category: Single protein]]
[[Category: Single protein]]
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[[Category: merohedral twinning]]
[[Category: merohedral twinning]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 17:25:56 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 16:57:47 2008''

Revision as of 14:57, 20 March 2008

Image:2fx3.gif


PDB ID 2fx3

Drag the structure with the mouse to rotate
, resolution 3.400Å
Ligands: and
Coordinates: save as pdb, mmCIF, xml



Crystal Structure Determination of E. coli Elongation Factor, Tu using a Twinned Data Set


Overview

Escherichia coli elongation factor Tu-GDP (EF-Tu-GDP) was crystallized in the presence of novel inhibitors. The only crystals which could be grown were epitaxially as well as merohedrally twinned, highly mosaic and diffracted to a resolution of 3.4 A in space group P3(1)21, with unit-cell parameters a = b = 69.55, c = 169.44 A, alpha = beta = 90, gamma = 120 degrees . To determine whether an inhibitor was present in the crystal, a poor-quality X-ray diffraction data set had to be processed. The three-dimensional structure was ultimately solved and the original question answered. The results also reveal a new type of dimer packing for EF-Tu-GDP.

About this Structure

2FX3 is a Single protein structure of sequence from Escherichia coli. Full crystallographic information is available from OCA.

Reference

Solving the structure of Escherichia coli elongation factor Tu using a twinned data set., Heffron SE, Moeller R, Jurnak F, Acta Crystallogr D Biol Crystallogr. 2006 Apr;62(Pt 4):433-8. Epub 2006, Mar 18. PMID:16552145

Page seeded by OCA on Thu Mar 20 16:57:47 2008

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