2fym
From Proteopedia
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| - | [[Image:2fym.gif|left|200px]] | + | [[Image:2fym.gif|left|200px]] |
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| - | '''Crystal structure of E. coli enolase complexed with the minimal binding segment of RNase E.''' | + | {{Structure |
| + | |PDB= 2fym |SIZE=350|CAPTION= <scene name='initialview01'>2fym</scene>, resolution 1.60Å | ||
| + | |SITE= | ||
| + | |LIGAND= <scene name='pdbligand=MG:MAGNESIUM ION'>MG</scene> | ||
| + | |ACTIVITY= [http://en.wikipedia.org/wiki/Phosphopyruvate_hydratase Phosphopyruvate hydratase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=4.2.1.11 4.2.1.11] | ||
| + | |GENE= eno ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=562 Escherichia coli]) | ||
| + | }} | ||
| + | |||
| + | '''Crystal structure of E. coli enolase complexed with the minimal binding segment of RNase E.''' | ||
| + | |||
==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
| - | 2FYM is a [ | + | 2FYM is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2FYM OCA]. |
==Reference== | ==Reference== | ||
| - | Recognition of enolase in the Escherichia coli RNA degradosome., Chandran V, Luisi BF, J Mol Biol. 2006 Apr 21;358(1):8-15. Epub 2006 Feb 21. PMID:[http:// | + | Recognition of enolase in the Escherichia coli RNA degradosome., Chandran V, Luisi BF, J Mol Biol. 2006 Apr 21;358(1):8-15. Epub 2006 Feb 21. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/16516921 16516921] |
[[Category: Escherichia coli]] | [[Category: Escherichia coli]] | ||
[[Category: Phosphopyruvate hydratase]] | [[Category: Phosphopyruvate hydratase]] | ||
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[[Category: rnase e]] | [[Category: rnase e]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 16:58:19 2008'' |
Revision as of 14:58, 20 March 2008
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| , resolution 1.60Å | |||||||
|---|---|---|---|---|---|---|---|
| Ligands: | |||||||
| Gene: | eno (Escherichia coli) | ||||||
| Activity: | Phosphopyruvate hydratase, with EC number 4.2.1.11 | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
Crystal structure of E. coli enolase complexed with the minimal binding segment of RNase E.
Overview
In Escherichia coli, the glycolytic enzyme enolase is a component of the RNA degradosome, which is an RNase E mediated assembly involved in RNA processing and transcript turnover. The recruitment of enolase by the RNA degradosome has been implicated in the turnover of certain transcripts, and it is mediated by a small segment of roughly a dozen residues that lie within a natively unstructured sub-domain of RNase E. Here, we present the crystal structure of enolase in complex with its recognition site from RNase E at 1.6A resolution. A single molecule of the RNase E peptide binds asymmetrically in a conserved cleft at the interface of the enolase dimer. The recognition site is well conserved in RNase E homologues in a subfamily of the gamma-proteobacteria, including enzymes from pathogens such as Yersinia pestis, Vibrio cholera and Salmonella sp. We suggest that enolase is recruited into putative RNA degradosome machinery in these bacilli, where it plays common regulatory functions.
About this Structure
2FYM is a Protein complex structure of sequences from Escherichia coli. Full crystallographic information is available from OCA.
Reference
Recognition of enolase in the Escherichia coli RNA degradosome., Chandran V, Luisi BF, J Mol Biol. 2006 Apr 21;358(1):8-15. Epub 2006 Feb 21. PMID:16516921
Page seeded by OCA on Thu Mar 20 16:58:19 2008
