2g0b
From Proteopedia
| Line 1: | Line 1: | ||
| - | [[Image:2g0b.gif|left|200px]] | + | [[Image:2g0b.gif|left|200px]] |
| - | + | ||
| - | '''The structure of FeeM, an N-acyl amino acid synthase from uncultured soil microbes''' | + | {{Structure |
| + | |PDB= 2g0b |SIZE=350|CAPTION= <scene name='initialview01'>2g0b</scene>, resolution 3.000Å | ||
| + | |SITE= | ||
| + | |LIGAND= <scene name='pdbligand=NLT:N-DODECANOYL-L-TYROSINE'>NLT</scene> | ||
| + | |ACTIVITY= | ||
| + | |GENE= feem ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=77133 uncultured bacterium]) | ||
| + | }} | ||
| + | |||
| + | '''The structure of FeeM, an N-acyl amino acid synthase from uncultured soil microbes''' | ||
| + | |||
==Overview== | ==Overview== | ||
| Line 7: | Line 16: | ||
==About this Structure== | ==About this Structure== | ||
| - | 2G0B is a [ | + | 2G0B is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Uncultured_bacterium Uncultured bacterium]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2G0B OCA]. |
==Reference== | ==Reference== | ||
| - | FeeM, an N-acyl amino acid synthase from an uncultured soil microbe: structure, mechanism, and acyl carrier protein binding., Van Wagoner RM, Clardy J, Structure. 2006 Sep;14(9):1425-35. PMID:[http:// | + | FeeM, an N-acyl amino acid synthase from an uncultured soil microbe: structure, mechanism, and acyl carrier protein binding., Van Wagoner RM, Clardy J, Structure. 2006 Sep;14(9):1425-35. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/16962973 16962973] |
[[Category: Single protein]] | [[Category: Single protein]] | ||
[[Category: Uncultured bacterium]] | [[Category: Uncultured bacterium]] | ||
| Line 21: | Line 30: | ||
[[Category: protein-product complex]] | [[Category: protein-product complex]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 16:58:53 2008'' |
Revision as of 14:58, 20 March 2008
| |||||||
| , resolution 3.000Å | |||||||
|---|---|---|---|---|---|---|---|
| Ligands: | |||||||
| Gene: | feem (uncultured bacterium) | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
The structure of FeeM, an N-acyl amino acid synthase from uncultured soil microbes
Overview
Attempts to access antibiotics by capturing biosynthetic genes and pathways directly from environmental DNA, which is overwhelmingly derived from uncultured bacteria, have revealed a large and previously unknown family of N-acyl amino acid synthases (NASs). The structure of the NAS FeeM reveals structural similarity to the GCN5-related N-acyl transferases and acylhomoserine lactone synthases. The overall structure has a central beta sheet with alpha helices on both sides. A bound product at a cleft in the beta sheet identifies the active site and the structural basis for catalysis, and sequence conservation in this region indicates a bias for recognition over speed. FeeM interacts with an acyl carrier protein (FeeL), and the structure, mutagenesis, and enzymatic measurements reveal that a small hydrophobic pocket in alpha helix 5 dominates binding of FeeM to FeeL. The structural and mechanistic analyses suggest that the products of FeeM could be bacterial signaling agents.
About this Structure
2G0B is a Single protein structure of sequence from Uncultured bacterium. Full crystallographic information is available from OCA.
Reference
FeeM, an N-acyl amino acid synthase from an uncultured soil microbe: structure, mechanism, and acyl carrier protein binding., Van Wagoner RM, Clardy J, Structure. 2006 Sep;14(9):1425-35. PMID:16962973
Page seeded by OCA on Thu Mar 20 16:58:53 2008
