2g0l
From Proteopedia
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| - | [[Image:2g0l.gif|left|200px]] | + | [[Image:2g0l.gif|left|200px]] |
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| - | '''Solution Structure of Neocarzinostatin Apo-Protein with bound Flavone''' | + | {{Structure |
| + | |PDB= 2g0l |SIZE=350|CAPTION= <scene name='initialview01'>2g0l</scene> | ||
| + | |SITE= | ||
| + | |LIGAND= <scene name='pdbligand=FLN:2-PHENYL-4H-CHROMEN-4-ONE'>FLN</scene> | ||
| + | |ACTIVITY= | ||
| + | |GENE= ncsA ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1897 Streptomyces carzinostaticus]) | ||
| + | }} | ||
| + | |||
| + | '''Solution Structure of Neocarzinostatin Apo-Protein with bound Flavone''' | ||
| + | |||
==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
| - | 2G0L is a [ | + | 2G0L is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Streptomyces_carzinostaticus Streptomyces carzinostaticus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2G0L OCA]. |
==Reference== | ==Reference== | ||
| - | Synthetic ligands for apo-neocarzinostatin., Caddick S, Muskett FW, Stoneman RG, Woolfson DN, J Am Chem Soc. 2006 Apr 5;128(13):4204-5. PMID:[http:// | + | Synthetic ligands for apo-neocarzinostatin., Caddick S, Muskett FW, Stoneman RG, Woolfson DN, J Am Chem Soc. 2006 Apr 5;128(13):4204-5. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/16568976 16568976] |
[[Category: Single protein]] | [[Category: Single protein]] | ||
[[Category: Streptomyces carzinostaticus]] | [[Category: Streptomyces carzinostaticus]] | ||
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[[Category: igg fold]] | [[Category: igg fold]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 16:58:59 2008'' |
Revision as of 14:59, 20 March 2008
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| Ligands: | |||||||
| Gene: | ncsA (Streptomyces carzinostaticus) | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
Solution Structure of Neocarzinostatin Apo-Protein with bound Flavone
Overview
Neocarzinostatin (NCS) is a 1:1 complex of an enediyne chromophore (NCSChrom), non-covalently bound to an 11 kDa protein (apoNCS). We are exploring apoNCS as a generic protein system for sequestering small molecules for therapeutic applications. Here, we disclose a new flavone ligand 1 for apoNCS and present a high-resolution NMR structure of this ligand bound to apoNCS. This is the first high-resolution structure of a completely non-cognate ligand bound to the apoNCS protein. This work provides unambiguous evidence that a completely new class of ligand can bind specifically to apoNCS. Furthermore, the mode of binding is different than that of the naphthoate-based ligands, and for such a simple hydrophobic compound, the new ligand surprisingly binds specifically. This work indicates that apo-Neocarzinostatin has multiple selective and distinct binding modes for small-molecule cargo.
About this Structure
2G0L is a Single protein structure of sequence from Streptomyces carzinostaticus. Full crystallographic information is available from OCA.
Reference
Synthetic ligands for apo-neocarzinostatin., Caddick S, Muskett FW, Stoneman RG, Woolfson DN, J Am Chem Soc. 2006 Apr 5;128(13):4204-5. PMID:16568976
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